Abstract:
:Restriction fragment patterns of G+C-rich satellites of sheep and goat DNA were compared. The 1,712 g/cm3 satellites of both species appear homologous, consisting of repeats 760 base pairs long and showing coincidence of position of primary+ EcoRI, BamHI and most BspRI restriction target sites. The EcoRI and BamHI endonucleases produce mostly monomers of the repeating unit, while oligomers prevail in the A1uI and Bg1II digests. Species-specific differences in the frequency, position and mode of distribution of secondary+ restriction target sites for EcoRI, Bg1II and A1uI were observed. Unlike the 1,712 g/cm3 satellites, the 1,723 g/cm3 component of sheep DNA and the 1,719 g/cm3 material from goat DNA appear species--specific, since no homologous material could ever be detected in the DNA of the other species.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Forstová J,Votavová H,Guttmann T,Pivec L,Doskocil Jdoi
10.1093/nar/6.1.57subject
Has Abstractpub_date
1979-01-01 00:00:00pages
57-70issue
1eissn
0305-1048issn
1362-4962journal_volume
6pub_type
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