Abstract:
:Alfalfa mosaic virus RNA 4, the subgenomic messenger for viral coat protein, was partially digested with RNase T1 or RNase A and the sequence of a number of fragments was deduced by in vitro labeling with polynucleotide kinase and application of RNA sequencing techniques. From overlapping fragments, the complete primary sequence of the 881 nucleotides of RNA 4 was constructed: the coding region of 660 nucleotides (not including the initiation and termination codon) is flanked by a 5' noncoding region of 39 nucleotides and a 3' noncoding region of 182 nucleotides. The RNA sequencing data completely confirm the amino acid sequence of the coat protein as deduced by Van Beynum et al. (Fur.J. Biochem. 72, 63-78, 1977).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Brederode FT,Koper-Zwarthoff EC,Bol JFdoi
10.1093/nar/8.10.2213subject
Has Abstractpub_date
1980-05-24 00:00:00pages
2213-23issue
10eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract::Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Here we report the interesting phenomenon that puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.5.1176
更新日期:2000-03-01 00:00:00
abstract::Nematode.net (http://nematode.net) is a publicly available resource dedicated to the study of parasitic nematodes. In 2000, the Genome Center at Washington University (GC) joined a consortium including the Nematode Genomics group in Edinburgh, and the Pathogen Sequencing Unit of the Sanger Institute to generate expres...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn744
更新日期:2009-01-01 00:00:00
abstract::This article presents W-IQ-TREE, an intuitive and user-friendly web interface and server for IQ-TREE, an efficient phylogenetic software for maximum likelihood analysis. W-IQ-TREE supports multiple sequence types (DNA, protein, codon, binary and morphology) in common alignment formats and a wide range of evolutionary ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw256
更新日期:2016-07-08 00:00:00
abstract::RNArchitecture is a database that provides a comprehensive description of relationships between known families of structured non-coding RNAs, with a focus on structural similarities. The classification is hierarchical and similar to the system used in the SCOP and CATH databases of protein structures. Its central leve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx966
更新日期:2018-01-04 00:00:00
abstract::The mode of action of regulated promoters is largely determined by kinetic parameters which govern the interaction between promoters and proteins involved in induction and repression of transcription. To gain insight into the interplay between positively and negatively acting transcriptional regulators, in this case A...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.18.3873
更新日期:2001-09-15 00:00:00
abstract::PRINTS is a database of protein family 'fingerprints' offering a diagnostic resource for newly-determined sequences. By contrast with PROSITE, which uses single consensus expressions to characterise particular families, PRINTS exploits groups of motifs to build characteristic signatures. These signatures offer improve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.1.304
更新日期:1998-01-01 00:00:00
abstract::In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks164
更新日期:2012-06-01 00:00:00
abstract::Infection of cultured cells with herpes simplex virus (HSV) results in the transcriptional induction of a small number of cellular genes. Although the majority of such genes are dependent upon viral protein synthesis for their induction, a small minority are not. These genes are induced by events occurring prior to th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.23.9261
更新日期:1986-12-09 00:00:00
abstract::We have previously isolated two different aFGF cDNA clones from kidney and brain. The two corresponding mRNA, designated aFGF 1.A and 1.B, are the predominant species in kidney and brain, respectively. During the characterization of aFGF mRNA in glioblastoma cells, we demonstrated that aFGF mRNA in U1242MG and D65MG g...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.3.489
更新日期:1993-02-11 00:00:00
abstract::The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely rela...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl892
更新日期:2006-01-01 00:00:00
abstract::We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated DNA. To study the effects of the phosphorylation/dephosphorylation of MDBP-2-H1 on the expression of the avian vitellogeni...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.10.1816
更新日期:1996-05-15 00:00:00
abstract::Cationic aromatic tricyclic compounds including triphenylmethane dyes, phenazines, phenoxazines, acridines, phenothiazines, phenanthridinium compounds, anthracenes and xanthene dyes, which amplify cell killing in phleomycin-treated Escherichia coli B cells also modified phleomycin-induced breakdown of DNA to acid-solu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.23.9083
更新日期:1984-12-11 00:00:00
abstract::High-resolution structures of proteins remain the most valuable source for understanding their function in the cell and provide leads for drug design. Since the availability of sufficient protein structures to tackle complex problems such as modeling backbone moves or docking remains a problem, alternative approaches ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq972
更新日期:2011-01-01 00:00:00
abstract::Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. He...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv614
更新日期:2015-10-30 00:00:00
abstract::The hypothesis that DNA strands complementary to the coding strand contain in phase coding sequences has been investigated. Statistical analysis of the 50 genes of bacteriophage T7 shows no significant correlation between patterns of codon usage on the coding and non-coding strands. In Bacillus and yeast genes the cor...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.4.1389
更新日期:1985-02-25 00:00:00
abstract::Single cell RNA-seq experiments provide valuable insight into cellular heterogeneity but suffer from low coverage, 3' bias and technical noise. These unique properties of single cell RNA-seq data make study of alternative splicing difficult, and thus most single cell studies have restricted analysis of transcriptome v...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1525
更新日期:2016-05-05 00:00:00
abstract::The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.11.1549
更新日期:1974-11-01 00:00:00
abstract::Bulk replicative DNA synthesis in eukaryotes is highly accurate and efficient, primarily because of two DNA polymerases (Pols): Pols δ and ε. The high fidelity of these enzymes is due to their intrinsic base selectivity and proofreading exonuclease activity which, when coupled with post-replication mismatch repair, he...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1034
更新日期:2011-03-01 00:00:00
abstract::Assembly factors provide speed and directionality to the maturation process of the 30S subunit in bacteria. To gain a more precise understanding of how these proteins mediate 30S maturation, it is important to expand on studies of 30S assembly intermediates purified from bacterial strains lacking particular maturation...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz571
更新日期:2019-09-05 00:00:00
abstract::The Mouse Genome Database (MGD) (http://www.informatics.jax.org) is the community model organism database resource for the laboratory mouse, a premier animal model for the study of genetic and genomic systems relevant to human biology and disease. MGD maintains a comprehensive catalog of genes, functional RNAs and oth...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1225
更新日期:2014-01-01 00:00:00
abstract::NF-IL6beta regulates gene expression and plays function roles in many tissues. The EGF-regulated cyclooxygenase-2 (cox-2) expression is mediated through p38(MAPK) signaling pathway and positively correlates with NF-IL6beta expression in A431 cells. NF-IL6beta coordinated with c-Jun on cox-2 transcriptional activation ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj422
更新日期:2006-01-05 00:00:00
abstract::Hidden Markov models (HMMs) and their variants are widely used in Bioinformatics applications that analyze and compare biological sequences. Designing a novel application requires the insight of a human expert to define the model's architecture. The implementation of prediction algorithms and algorithms to train the m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp662
更新日期:2009-11-01 00:00:00
abstract::Non-homologous end-joining (NHEJ) of DNA double-strand breaks (DSBs) is mediated by two protein complexes comprising Ku80/Ku70/DNA-PKcs/Artemis and XRCC4/LigaseIV/XLF. Loss of Ku or XRCC4/LigaseIV function compromises the rejoining of radiation-induced DSBs and leads to defective V(D)J recombination. In this study, we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn094
更新日期:2008-05-01 00:00:00
abstract::An investigation of the mobility of short duplexes of DNA -octamers and decamers-on polyacrylamide gels is presented, showing that molecules containing less than one helical turn exhibit sequence dependent mobilities. Analysis of chains with different sequences indicates that any arrangement of two or more adjacent A....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.14.6803
更新日期:1988-07-25 00:00:00
abstract::PALI (release 1.2) contains three-dimensional (3-D) structure-dependent sequence alignments as well as structure-based phylogenetic trees of homologous protein domains in various families. The data set of homologous protein structures has been derived by consulting the SCOP database (release 1.50) and the data set com...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.61
更新日期:2001-01-01 00:00:00
abstract::The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.14.3887
更新日期:1991-07-25 00:00:00
abstract::After ionising radiation double-strand breaks (dsb) are lethal if not repaired or misrepaired. Cell killing is greatly enhanced by hyperthermia and it is questioned here whether heat not only affects dsb repair capacity but also fidelity in a chromosomal context. dsb repair experiments were designed so as to mainly sc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.9.1960
更新日期:2001-05-01 00:00:00
abstract::Hepatitis C virus (HCV) translation initiation is directed by an internal ribosome entry site (IRES) and regulated by distant regions at the 3'-end of the viral genome. Through a combination of improved RNA chemical probing methods, SHAPE structural analysis and screening of RNA accessibility using antisense oligonucl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks927
更新日期:2012-12-01 00:00:00
abstract::The DNA sequence of a 225.4 kilobase segment of the Escherichia coli K-12 genome is described here, from 76.0 to 81.5 minutes on the genetic map. This brings the total of contiguous sequence from the E.coli genome project to 725.1 kb (76.0 to 92.8 minutes). We found 191 putative coding genes (ORFs) of which 72 genes w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.13.2576
更新日期:1994-07-11 00:00:00
abstract::During the formation of a transcriptionally active macronucleus, ciliated protozoa excise large numbers of interstitial segments of DNA (internal eliminated sequences; IESs) from their chromosomes. In this study we analyze the published sequences of 20 IESs that interrupt surface protein genes of Paramecium and identi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.11.2006
更新日期:1995-06-11 00:00:00