3D-Image analysis platform monitoring relocation of pluripotency genes during reprogramming.

abstract：:We report here the construction of engineered endonuclease database (EENdb) (http://eendb.zfgenetics.org/), a searchable database and knowledge base for customizable engineered endonucleases (EENs), including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). EENs are artificial...

journal_title：Nucleic acids research

authors： Xiao A,Wu Y,Yang Z,Hu Y,Wang W,Zhang Y,Kong L,Gao G,Zhu Z,Lin S,Zhang B

更新日期：2013-01-01 00:00:00

abstract：:Cis-elements (-933 to -641) upstream of the human M creatine kinase gene cap site contain an enhancer that confers developmental and tissue-specific expression to the chloramphenicol acetyltransferase gene in C2C12 myogenic cells transfected in culture. Division of the enhancer at -770 into a 5' fragment that includes...

journal_title：Nucleic acids research

authors： Trask RV,Koster JC,Ritchie ME,Billadello JJ

更新日期：1992-05-11 00:00:00

abstract：:Genes in the germline (micronuclear) genome of hypotrichous ciliates are interrupted by multiple, short, non-coding, AT-rich sequences called internal eliminated segments, or IESs. During conversion of a micronucleus to a somatic nucleus (macronucleus) after cell mating, all IESs are excised from the germline genes an...

journal_title：Nucleic acids research

authors： Prescott DM

更新日期：1999-03-01 00:00:00

abstract：:Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 phi2.5 promoter. Efficient fluorophore labeling of 5' RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current...

journal_title：Nucleic acids research

authors： Li N,Yu C,Huang F

更新日期：2005-02-24 00:00:00

abstract：:PlantGDB (http://www.plantgdb.org/) is a genomics database encompassing sequence data for green plants (Viridiplantae). PlantGDB provides annotated transcript assemblies for >100 plant species, with transcripts mapped to their cognate genomic context where available, integrated with a variety of sequence analysis tool...

journal_title：Nucleic acids research

authors： Duvick J,Fu A,Muppirala U,Sabharwal M,Wilkerson MD,Lawrence CJ,Lushbough C,Brendel V

更新日期：2008-01-01 00:00:00

abstract：:The interpretation of data-driven experiments in genomics often involves a search for biological categories that are enriched for the responder genes identified by the experiments. However, knowledge bases such as the Gene Ontology (GO) contain hundreds or thousands of categories with very high overlap between categor...

journal_title：Nucleic acids research

authors： Bauer S,Gagneur J,Robinson PN

更新日期：2010-06-01 00:00:00

abstract：:A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are generated between PCR products and a PCR-amplified plasmid vector. The procedure does not require the use of restriction enzymes, T4 DNA ligase or ...

journal_title：Nucleic acids research

authors： Aslanidis C,de Jong PJ

更新日期：1990-10-25 00:00:00

abstract：:RNA is ideally suited for in vitro evolution experiments, because a single RNA molecule possesses both genotypic (replicable sequence) and phenotypic (selectable shape) properties. Using systematic evolution of ligands by exponential enrichment (SELEX), we found a single 58-nt aptamer sequence that assumes two structu...

journal_title：Nucleic acids research

authors： Huang Z,Pei W,Han Y,Jayaseelan S,Shekhtman A,Shi H,Niu L

更新日期：2009-07-01 00:00:00

abstract：:Antibodies to the deoxyribotrinucleotides dpApTpA and dpApApT were prepared by injecting the bovine serum albumin conjugates of the respective haptens in rabbits. The specificities of the antibodies were determined by estimating the inhibition of the binding of the tritiated haptens to the immunoglobulins by various n...

journal_title：Nucleic acids research

authors： Khan SA,Jacob TM

更新日期：1977-09-01 00:00:00

abstract：:We have compiled the DNA sequence data for E. coli K12 available from the GENBANK and EMBO databases and over a period of several years independently from the literature. We have introduced all available genetic map data and have arranged the sequences accordingly. As far as possible the overlaps are deleted and a tot...

journal_title：Nucleic acids research

authors： Kröger M

更新日期：1989-01-01 00:00:00

abstract：:Synthetic DNA fragments were constructed to determine the effect of G tracts, in conjunction with periodically spaced A tracts, on DNA bends. Relative length measurements showed that the G tracts spaced at the half helical turn enhanced the DNA bend. When the G tract was interrupted with a thymine or shortened to one ...

journal_title：Nucleic acids research

authors： Milton DL,Casper ML,Wills NM,Gesteland RF

更新日期：1990-02-25 00:00:00

abstract：:REPPER (REPeats and their PERiodicities) is an integrated server that detects and analyzes regions with short gapless repeats in protein sequences or alignments. It finds periodicities by Fourier Transform (FTwin) and internal similarity analysis (REPwin). FTwin assigns numerical values to amino acids that reflect cer...

journal_title：Nucleic acids research

authors： Gruber M,Söding J,Lupas AN

更新日期：2005-07-01 00:00:00

abstract：:Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease di...

journal_title：Nucleic acids research

authors： Truelsen E,Gausing K,Jochimsen B,Jørgensen P,Marcker KA

更新日期：1979-07-11 00:00:00

abstract：:Biological networks play increasingly important roles in omics data integration and systems biology. Over the past decade, many excellent tools have been developed to support creation, analysis and visualization of biological networks. However, important limitations remain: most tools are standalone programs, the majo...

journal_title：Nucleic acids research

authors： Zhou G,Xia J

更新日期：2018-07-02 00:00:00

abstract：:Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...

journal_title：Nucleic acids research

authors： Schäfer M,Kunz W

更新日期：1985-02-25 00:00:00

abstract：:The triphosphate of the nucleoside deoxyribosyl dihydropyrimido[4,5-c][1,2]oxazin-7-one (dP) is known to be incorporated into DNA efficiently by Taq polymerase and is a useful tool for polymerase-mediated in vitro mutagenesis. It is shown here that dP is a potent mutagen in Escherichia coli and Salmonella typhimurium ...

journal_title：Nucleic acids research

authors： Negishi K,Williams DM,Inoue Y,Moriyama K,Brown DM,Hayatsu H

更新日期：1997-04-15 00:00:00

abstract：:Protein-induced DNA bending is of importance in the formation of complex nucleoprotein assemblies such as those involved in the initiation of DNA replication or transcription initiation. We have compared the DNA bending characteristics of the Escherichia coli cyclic AMP receptor protein (CRP or CAP), an archetypal DNA...

journal_title：Nucleic acids research

authors： Gaston K,Bell A,Busby S,Fried M

更新日期：1992-07-11 00:00:00

abstract：:A new procedure for the purification of B. subtilis RNA polymerase, based on mild lysis of cells, low speed centrifugation, gel filtration, DEAE-Sephadex chromatography and affinity chromatography on DNA-cellulose, yields three forms of enzyme referred here as enzyme A, B and C. As revealed by SDS gel electrophoresis,...

journal_title：Nucleic acids research

authors： Plevan P,Albertini AM,Galizzi A,Adamoli A,Mastromei G,Riva S,Cassani G

更新日期：1977-03-01 00:00:00

abstract：:The U1 snRNP-specific 70K protein is one of the few snRNP proteins from higher eukaryotic cells that is phosphorylated in vivo (1,2). Immunoaffinity purified spliceosomal snRNPs (U1, U2, U5, and U4/U6) were tested for their ability to phosphorylate in vitro the U1-specific 70K protein. An snRNP-associated kinase activ...

journal_title：Nucleic acids research

authors： Woppmann A,Will CL,Kornstädt U,Zuo P,Manley JL,Lührmann R

更新日期：1993-06-25 00:00:00

abstract：:Bursts of free radicals produced by ionization of water in close vicinity to DNA can produce clusters of opposed DNA lesions and these are termed multiply damaged sites (MDS). How MDS are processed by the Escherichia coli DNA glycosylases, endonuclease (endo) III and endo VIII, which recognize oxidized pyrimidines, is...

journal_title：Nucleic acids research

authors： Harrison L,Hatahet Z,Purmal AA,Wallace SS

更新日期：1998-02-15 00:00:00

abstract：:The pyrrole-imidazole (Py-Im) triamide-cyclopropa pyrroloindole (CPI) conjugates ImPyImLDu86 (7) and ImImPyLDu86 (14) were synthesized and their alkylating activities and inhibitory effects on DNA hydrolysis by restriction endonucleases were examined. Sequencing gel analysis demonstrated that conjugates 7 and 14 speci...

journal_title：Nucleic acids research

authors： Fujimoto K,Iida H,Kawakami M,Bando T,Tao ZF,Sugiyama H

更新日期：2002-09-01 00:00:00

abstract：:UV-irradiation (lambda = 254 nm) of liquid aqueous solutions of deoxyguanosine in the presence of oxygen at pH less than 7 causes an intensive degradation of nucleoside. The quantum yield estimated from A254 decrease for the reaction mixture was found to be 1.5X10(-4) at doses to 150 E/mole. The rate of A254 decrease ...

journal_title：Nucleic acids research

authors： Ivanchenko VA,Budowsky EI,Simukova NA,Vul'fson NS,Tishchenko AI,Askerov DB

更新日期：1977-04-01 00:00:00

abstract：:Using a human interferon-alpha probe we have isolated recombinant phages containing murine interferon-alpha (Mu IFN-alpha) genes from a genomic library. One of these phages contained two complete Mu IFN-alpha genes and part of a third gene. The insert of a second phage held two IFN genes. This indicates that the Mu IF...

journal_title：Nucleic acids research

authors： Zwarthoff EC,Mooren AT,Trapman J

更新日期：1985-02-11 00:00:00

abstract：:The TcSNP database (http://snps.tcruzi.org) integrates information on genetic variation (polymorphisms and mutations) for different stocks, strains and isolates of Trypanosoma cruzi, the causative agent of Chagas disease. The database incorporates sequences (genes from the T. cruzi reference genome, mRNAs, ESTs and ge...

journal_title：Nucleic acids research

authors： Ackermann AA,Carmona SJ,Agüero F

更新日期：2009-01-01 00:00:00

abstract：:A DNA methylase has been purified 270-fold from HeLa cell nuclei by chromatography on DEAE-cellulose, phosphocellulose, and hydroxyapatite. The enzyme transfers methyl groups from S-adenosyl-L-methionine to cytosine residues in DNA. The sole product of the reaction has been identified as 5-methylcytosine. The enzyme i...

journal_title：Nucleic acids research

authors： Roy PH,Weissbach A

更新日期：1975-10-01 00:00:00

abstract：:MobA protein, encoded by the broad host-range plasmid R1162, is required for conjugal mobilization of this plasmid. The protein is an essential part of the relaxosome, and is also necessary for the termination of strand transfer. In vitro, MobA is a nuclease specific for one of the two DNA strands of the origin of tra...

journal_title：Nucleic acids research

authors： Bhattacharjee MK,Meyer RJ

更新日期：1993-09-25 00:00:00

abstract：:DNA helicases are required for virtually every aspect of DNA metabolism, including replication, repair, recombination and transcription. A comprehensive description of these essential biochemical processes requires detailed understanding of helicase mechanisms. These enzymes are ubiquitous, having been identified in v...

journal_title：Nucleic acids research

authors： Mackintosh SG,Raney KD

更新日期：2006-01-01 00:00:00

abstract：:The binding of nine aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600 was measured and compared with the binding of the cognate amino acids. It was found that they bind rather tightly to the enzyme, the Kd's ranging from 3.1.10(-4) M with glycinol-AMP ester to 3.7.10(-9) M with L-isoleuc...

journal_title：Nucleic acids research

authors： Flossdorf J,Marutzky R,Messer K,Kula MR

更新日期：1977-03-01 00:00:00

abstract：:Transcription of transfer RNA genes by RNA polymerase III (Pol III) is controlled by general factors, TFIIIB and TFIIIC, and a negative regulator, Maf1. Here we report the interplay between TFIIIC and Maf1 in controlling Pol III activity upon the physiological switch of yeast from fermentation to respiration. TFIIIC d...

journal_title：Nucleic acids research

authors： Ciesla M,Skowronek E,Boguta M

更新日期：2018-10-12 00:00:00

abstract：:Mutations in the gene of human RNase T2 are associated with white matter disease of the human brain. Although brain abnormalities (bilateral temporal lobe cysts and multifocal white matter lesions) and clinical symptoms (psychomotor impairments, spasticity and epilepsy) are well characterized, the pathomechanism of RN...

journal_title：Nucleic acids research

authors： Thorn A,Steinfeld R,Ziegenbein M,Grapp M,Hsiao HH,Urlaub H,Sheldrick GM,Gärtner J,Krätzner R

更新日期：2012-09-01 00:00:00