Abstract:
:Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 phi2.5 promoter. Efficient fluorophore labeling of 5' RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current work makes fluorescently labeled RNA readily available for broad applications in biochemistry, molecular biology, structural biology and biomedicine. In particular, site-specifically fluorophore-labeled large RNAs prepared by the current method may be used to investigate RNA structure, folding and mechanism by various fluorescence techniques. In addition, F550/570 and F650/670 may replace [gamma-32P]ATP to prepare 5' labeled RNA for RNA structural and functional investigation, thereby eliminating the need for the unstable and radio-hazardous [gamma-32P]ATP.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Li N,Yu C,Huang Fdoi
10.1093/nar/gni036keywords:
subject
Has Abstractpub_date
2005-02-24 00:00:00pages
e37issue
4eissn
0305-1048issn
1362-4962pii
33/4/e37journal_volume
33pub_type
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