Abstract:
:In Trypanosoma brucei the genes are organised into long polycistronic transcription units and only three promoters for protein-encoding genes and a single terminator have been characterised. These promoters recruit a polI-like RNA polymerase for the transcription units encoding the two major stage-specific antigens of the parasite, the variant surface glycoprotein (VSG) of the bloodstream form and procyclin of the insect-specific procyclic form, while the terminator is that of a procyclin transcription unit. By deletional and mutational analysis we defined the two DNA sequences essential for the activity of the VSG promoter from a bloodstream form transcription unit and one of the functional elements of the procyclin terminator. These three short sequences are similar, and their C-rich strand binds the same protein of 40 kDa. In addition, this factor also binds to the C-rich strand of the telomeric repeats, the consensus target sequence being 5'-CCCTNN-3'. The factor-binding sequences are functionally interchangeable in chimeric promoter or terminator constructs, although additional elements are required for full activity.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Berberof M,Vanhamme L,Alexandre S,Lips S,Tebabi P,Pays Edoi
10.1093/nar/28.2.597keywords:
subject
Has Abstractpub_date
2000-01-15 00:00:00pages
597-604issue
2eissn
0305-1048issn
1362-4962pii
gkd136journal_volume
28pub_type
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