Abstract：
:ETS-domain transcription factors play important roles in controlling gene expression in a variety of different contexts; however, these proteins bind to very similar sites and it is unclear how in vivo specificity is achieved. In silico analysis is unlikely to reveal specific targets for individual family members and direct experimental approaches are therefore required. Here, we take advantage of an inducible dominant-negative expression system to identify a group of novel target genes for the ETS-domain transcription factor Elk-1. Elk-1 is thought to mainly function through cooperation with a second transcription factor SRF, but the targets we identify are largely SRF-independent. Furthermore, we demonstrate that there is a high degree of overlapping, cell type-specific, target gene binding by Elk-1 and other ETS-domain transcription factors. Our results are therefore consistent with the notion that there is a high degree of functional redundancy in target gene regulation by ETS-domain transcription factors in addition to the specific target gene regulation that can be dictated through heterotypic interactions exemplified by the Elk-1-SRF complex.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Boros J,O'Donnell A,Donaldson IJ,Kasza A,Zeef L,Sharrocks ADdoi
10.1093/nar/gkp804subject
Has Abstractpub_date
2009-12-01 00:00:00pages
7368-80issue
22eissn
0305-1048issn
1362-4962pii
gkp804journal_volume
37pub_type
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