Abstract:
:Hepatitis B virus (HBV) infection remains a major health problem worldwide. The role played by microRNAs (miRNAs) in HBV replication and pathogenesis is being increasingly recognized. In this study, we found that miR-15b, an important miRNA during HBV infection and hepatocellular carcinoma development, directly binds hepatocyte nuclear factor 1α (HNF1α) mRNA, a negative regulator of HBV Enhancer I, to attenuate HNF1α expression, resulting in transactivation of HBV Enhancer I, in turn causing the enhancement of HBV replication and expression of HBV antigens, including HBx protein, finally leading to the down-regulated expression of miR-15b in both cell lines and mice in a long cascade of events. Our research showed that miR-15b promotes HBV replication by augmenting HBV Enhancer I activity via direct targeting HNF1α, while HBV replication and antigens expression, particularly the HBx protein, then repress the expression of miR-15b. The reciprocal regulation between miR-15b and HBV controls the level of HBV replication and might play a role in persistent HBV infection. This work adds to the body of knowledge concerning the complex interactions between HBV and host miRNAs.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Dai X,Zhang W,Zhang H,Sun S,Yu H,Guo Y,Kou Z,Zhao G,Du L,Jiang S,Zhang J,Li J,Zhou Ydoi
10.1093/nar/gku260subject
Has Abstractpub_date
2014-06-01 00:00:00pages
6578-90issue
10eissn
0305-1048issn
1362-4962pii
gku260journal_volume
42pub_type
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