Early melting of supercoiled DNA topoisomers observed by TGGE.

abstract：:We have sequenced 14 introns from the ciliate Tetrahymena thermophila and include these in an analysis of the 27 intron sequences available from seven T. thermophila protein-encoding genes. Consensus 5' and 3' splice junctions were determined and found to resemble the junctions of other nuclear pre-mRNA introns. Uniqu...

journal_title：Nucleic acids research

authors： Csank C,Taylor FM,Martindale DW

更新日期：1990-09-11 00:00:00

abstract：:We have developed an improved method for photofootprinting in vivo which utilizes the thermostable DNA polymerase from T. aquaticus (Taq) in a primer extension assay. UV light is used to introduce photoproducts into the genomic DNA of intact yeast cells. The photoproducts are then detected and mapped at the nucleotide...

journal_title：Nucleic acids research

authors： Axelrod JD,Majors J

更新日期：1989-01-11 00:00:00

abstract：:The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...

journal_title：Nucleic acids research

authors： Kreivi JP,Akusjärvi G

更新日期：1994-02-11 00:00:00

abstract：:We describe an algorithm for gene identification in DNA sequences derived from shotgun sequencing of microbial communities. Accurate ab initio gene prediction in a short nucleotide sequence of anonymous origin is hampered by uncertainty in model parameters. While several machine learning approaches could be proposed t...

journal_title：Nucleic acids research

authors： Zhu W,Lomsadze A,Borodovsky M

更新日期：2010-07-01 00:00:00

abstract：:The NHGRI-EBI GWAS Catalog has provided data from published genome-wide association studies since 2008. In 2015, the database was redesigned and relocated to EMBL-EBI. The new infrastructure includes a new graphical user interface (www.ebi.ac.uk/gwas/), ontology supported search functionality and an improved curation ...

journal_title：Nucleic acids research

authors： MacArthur J,Bowler E,Cerezo M,Gil L,Hall P,Hastings E,Junkins H,McMahon A,Milano A,Morales J,Pendlington ZM,Welter D,Burdett T,Hindorff L,Flicek P,Cunningham F,Parkinson H

更新日期：2017-01-04 00:00:00

abstract：:This paper describes the sequence of 257 nucleotides from the 3' end of RNA 2 of barley stripe mosaic virus ( BSMV , strain Argentina Mild) including an internal oligo (A) tract localized at a distance of 236 nucleotides from the 3' end, and the 3' terminal tRNA-like structure accepting tyrosine. This sequence is show...

journal_title：Nucleic acids research

authors： Kozlov YuV,Rupasov VV,Adyshev DM,Belgelarskaya SN,Agranovsky AA,Mankin AS,Morozov SYu,Dolja VV,Atabekov JG

更新日期：1984-05-11 00:00:00

abstract：:ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F(0)F(...

journal_title：Nucleic acids research

authors： Burger G,Lang BF,Braun HP,Marx S

更新日期：2003-05-01 00:00:00

abstract：:Tandem repeats in DNA have been under intensive study for many years, first, as a consequence of their usefulness as genomic markers and DNA fingerprints and more recently as their role in human disease and regulatory processes has become apparent. The Tandem Repeats Database (TRDB) is a public repository of informati...

journal_title：Nucleic acids research

authors： Gelfand Y,Rodriguez A,Benson G

更新日期：2007-01-01 00:00:00

abstract：:With the abundant mammalian lncRNAs identified recently, a comprehensive annotation resource for these novel lncRNAs is an urgent need. Since its first release in November 2016, AnnoLnc has been the only online server for comprehensively annotating novel human lncRNAs on-the-fly. Here, with significant updates to mult...

journal_title：Nucleic acids research

authors： Ke L,Yang DC,Wang Y,Ding Y,Gao G

更新日期：2020-07-02 00:00:00

abstract：:The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the m...

journal_title：Nucleic acids research

authors： Shashni R,Qayyum MZ,Vishalini V,Dey D,Sen R

更新日期：2014-09-01 00:00:00

abstract：:Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. He...

journal_title：Nucleic acids research

authors： Oomoto I,Suzuki-Hirano A,Umeshima H,Han YW,Yanagisawa H,Carlton P,Harada Y,Kengaku M,Okamoto A,Shimogori T,Wang DO

更新日期：2015-10-30 00:00:00

abstract：:Sensory photoreceptors have enabled non-invasive and spatiotemporal control of numerous biological processes. Photoreceptor engineering has expanded the repertoire beyond natural receptors, but to date no generally applicable strategy exists towards constructing light-regulated protein actuators of arbitrary function....

journal_title：Nucleic acids research

authors： Richter F,Fonfara I,Bouazza B,Schumacher CH,Bratovič M,Charpentier E,Möglich A

更新日期：2016-11-16 00:00:00

abstract：:Extensive efforts have been directed at the discovery, investigation and clinical monitoring of targeted therapeutics. These efforts may be facilitated by the convenient access of the genetic, proteomic, interactive and other aspects of the therapeutic targets. Here, we describe an update of the Therapeutic target dat...

journal_title：Nucleic acids research

authors： Li YH,Yu CY,Li XX,Zhang P,Tang J,Yang Q,Fu T,Zhang X,Cui X,Tu G,Zhang Y,Li S,Yang F,Sun Q,Qin C,Zeng X,Chen Z,Chen YZ,Zhu F

更新日期：2018-01-04 00:00:00

abstract：:G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during ...

journal_title：Nucleic acids research

authors： David AP,Margarit E,Domizi P,Banchio C,Armas P,Calcaterra NB

更新日期：2016-05-19 00:00:00

abstract：:GGRNA (http://GGRNA.dbcls.jp/) is a Google-like, ultrafast search engine for genes and transcripts. The web server accepts arbitrary words and phrases, such as gene names, IDs, gene descriptions, annotations of gene and even nucleotide/amino acid sequences through one simple search box, and quickly returns relevant Re...

journal_title：Nucleic acids research

authors： Naito Y,Bono H

更新日期：2012-07-01 00:00:00

abstract：:More than one-third of the RAG1 protein can be truncated from the N-terminus with only subtle effects on the products of V(D)J recombination in vitro or in a mouse. What, then, is the function of the N-terminal domain? We believe it to be regulatory. We determined, several years ago, that an included RING motif could ...

journal_title：Nucleic acids research

authors： Maitra R,Sadofsky MJ

更新日期：2009-06-01 00:00:00

abstract：:Friedreich ataxia (FRDA) is caused by hyperexpansion of GAA*TTC repeats located in the first intron of the FXN gene, which inhibits transcription leading to the deficiency of frataxin. The FXN gene is an excellent target for therapeutic intervention since (i) 98% of patients carry the same type of mutation, (ii) the m...

journal_title：Nucleic acids research

authors： Soragni E,Herman D,Dent SY,Gottesfeld JM,Wells RD,Napierala M

更新日期：2008-11-01 00:00:00

abstract：:The chemical reactivity of matched T and C bases to osmium tetroxide and hydroxylamine near mismatched and unmatched bases in a heteroduplex between two strands of DNA with multiple differences was examined. Data was available for matched bases one or two positions away from 24 mismatches. Reactive bases were found ne...

journal_title：Nucleic acids research

authors： Cotton RG,Campbell RD

更新日期：1989-06-12 00:00:00

abstract：:The potential use of alpha-beta-anomeric duplex oligonucleotides to inhibit transcription factor activity by the decoy approach is investigated in this report. Indeed, several alpha-beta-anomeric heteroduplexes display a sequence-specific interaction with the p50 subunit of the transcription factor NF kappa B. Used in...

journal_title：Nucleic acids research

authors： Tanaka H,Vickart P,Bertrand JR,Rayner B,Morvan F,Imbach JL,Paulin D,Malvy C

更新日期：1994-08-11 00:00:00

abstract：:A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtaine...

journal_title：Nucleic acids research

authors： Demidov V,Frank-Kamenetskii MD,Egholm M,Buchardt O,Nielsen PE

更新日期：1993-05-11 00:00:00

abstract：:To bridge the gap between the rising information needs of biological and medical researchers and the rapidly growing number of online bioinformatics resources, we have created the Online Bioinformatics Resources Collection (OBRC) at the Health Sciences Library System (HSLS) at the University of Pittsburgh. The OBRC, c...

journal_title：Nucleic acids research

authors： Chen YB,Chattopadhyay A,Bergen P,Gadd C,Tannery N

更新日期：2007-01-01 00:00:00

abstract：:The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) ...

journal_title：Nucleic acids research

authors： Fütterer J,Hohn T

更新日期：1992-08-11 00:00:00

abstract：:Triple helix-forming oligonucleotides covalently linked to topoisomerase I inhibitors, in particular the antitumor agent camptothecin, trigger topoisomerase I-mediated DNA cleavage selectively in the proximity of the binding site of the oligonucleotide vector. In the present study, we have performed a systematic analy...

journal_title：Nucleic acids research

authors： Arimondo PB,Angenault S,Halby L,Boutorine A,Schmidt F,Monneret C,Garestier T,Sun JS,Bailly C,Hélène C

更新日期：2003-07-15 00:00:00

abstract：:By successively assembling genetic parts such as BioBrick according to grammatical models, complex genetic constructs composed of dozens of functional blocks can be built. However, usually every category of genetic parts includes a few or many parts. With increasing quantity of genetic parts, the process of assembling...

journal_title：Nucleic acids research

authors： Fang G,Zhang S,Dong Y

更新日期：2017-12-15 00:00:00

abstract：:Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus RNA 3. The structure consists of two stem-loops separated by a single-stranded region. RNA 3-derived transcripts were ...

journal_title：Nucleic acids research

authors： Lauber E,Guilley H,Richards K,Jonard G,Gilmer D

更新日期：1997-12-01 00:00:00

abstract：:A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping. PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity. In the presence of an incomplete set of dNTPs, pfu was found to be extremely discriminative in nucleotide i...

journal_title：Nucleic acids research

authors： Hu YW,Balaskas E,Kessler G,Issid C,Scully LJ,Murphy DG,Rinfret A,Giulivi A,Scalia V,Gill P

更新日期：1998-11-01 00:00:00

abstract：:The circular dichroism spectra of Hg(II) complexes with native calf thymus DNA, chemically methylated Streptomyces chrysomallus DNA and with Ag(I)-DNA complexes were measured in the region of 220 - 340 nm. As a main result a conversion of the conservative CD spectrum of DNA to a distinct nonconservative type of CD spe...

journal_title：Nucleic acids research

authors： Walter A,Luck G

更新日期：1977-03-01 00:00:00

abstract：:DNA double stranded breaks (DSBs) are one of the most deleterious types of DNA lesions. The main pathways responsible for repairing these breaks in eukaryotic cells are homologous recombination (HR) and non-homologous end-joining (NHEJ). However, a third group of still poorly characterized DSB repair pathways, collect...

journal_title：Nucleic acids research

authors： Kostyrko K,Mermod N

更新日期：2016-04-07 00:00:00

abstract：:Restriction fragment patterns of G+C-rich satellites of sheep and goat DNA were compared. The 1,712 g/cm3 satellites of both species appear homologous, consisting of repeats 760 base pairs long and showing coincidence of position of primary+ EcoRI, BamHI and most BspRI restriction target sites. The EcoRI and BamHI end...

journal_title：Nucleic acids research

authors： Forstová J,Votavová H,Guttmann T,Pivec L,Doskocil J

更新日期：1979-01-01 00:00:00

abstract：:Frequencies for each of the 61 amino acid codons have been determined in every published mRNA sequence of 50 or more codons. The frequencies are shown for each kind of genome and for each individual gene. A surprising consistency of choices exists among genes of the same or similar genomes. Thus each genome, or kind o...

journal_title：Nucleic acids research

authors： Grantham R,Gautier C,Gouy M,Mercier R,Pavé A

更新日期：1980-01-11 00:00:00