Abstract：
:More than one-third of the RAG1 protein can be truncated from the N-terminus with only subtle effects on the products of V(D)J recombination in vitro or in a mouse. What, then, is the function of the N-terminal domain? We believe it to be regulatory. We determined, several years ago, that an included RING motif could function as an ubiquitin E3 ligase. Whether this activity is limited to automodification, or may alter other proteins in the cell, remains an open question. We revisited the issue of additional protein-protein interactions between RAG1 and other proteins by means of the yeast two-hybrid assay. We confirmed the interaction already described with KPNA2/RCH1/SRP1alpha and found two others--to the transcription factor GMEB1/PIF p96 and the splicing factor SF3A2/SF3a66. A luciferase reporter assay demonstrates that a protein complex containing RAG proteins and the transcription factor can assemble in cells. Further mapping identified a region within the N-terminal domain resembling a WW motif. Point mutation directed at residues conserved in WW motifs eliminated binding to one of the partners. Phylogenetic analysis shows the WW-like module to be highly conserved. The module contributes to protein-protein interactions that may also influence how RAG1 binds DNA targets.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Maitra R,Sadofsky MJdoi
10.1093/nar/gkp192subject
Has Abstractpub_date
2009-06-01 00:00:00pages
3301-9issue
10eissn
0305-1048issn
1362-4962pii
gkp192journal_volume
37pub_type
杂志文章abstract：:A previously elaborated technique for fixing a chosen partially melted state of DNA with glyoxal was used in a study of the melting process of the replicative form (RF III) of phi X174 DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoret...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.6.1867
更新日期：1982-03-25 00:00:00
abstract：:TOP mRNAs are vertebrate transcripts which contain a 5'terminal oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5'TOP. In the present study we show that elongation factor 2 (EF2) mRNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.5.995
更新日期：1997-03-01 00:00:00
abstract：:A class II site-specific endodeoxyribonuclease (SalPI) was identified in cell-free extracts of Streptomyces albus CMI 52766 after high speed centrifugation and fractionation through Bio Gel AO.5M. SalPI cleaves lambda DNA into at least 18 fragments. Five cleavage sites were located in the linear lambda map by the use ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.6.1989
更新日期：1977-06-01 00:00:00
abstract：:The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks198
更新日期：2012-07-01 00:00:00
abstract：:Sox proteins belong to the HMG box superfamily of DNA-binding proteins and are found throughout the animal kingdom. They are involved in the regulation of such diverse developmental processes as germ layer formation, organ development and cell type specifi-cation. Hence, deletion or mutation of Sox proteins often resu...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/27.6.1409
更新日期：1999-03-15 00:00:00
abstract：:Restriction endonuclease Bse634I recognizes and cleaves the degenerate DNA sequence 5'-R/CCGGY-3' (R stands for A or G; Y for T or C, '/' indicates a cleavage position). Here, we report the crystal structures of the Bse634I R226A mutant complexed with cognate oligoduplexes containing ACCGGT and GCCGGC sites, respectiv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks300
更新日期：2012-08-01 00:00:00
abstract：:Lens epithelium-derived growth factor/p75 (LEDGF/p75) is a transcriptional coactivator involved in stress response, autoimmune disease, cancer and HIV replication. A fusion between the nuclear pore protein NUP98 and LEDGF/p75 has been found in human acute and chronic myeloid leukemia and association of LEDGF/p75 with ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq410
更新日期：2010-10-01 00:00:00
abstract：:Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNA-like (C3'-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself. We performed molecular dynamics simulations on five isosequential duplexes (LNA-DNA, LNA-LNA, LNA-R...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1182
更新日期：2008-03-01 00:00:00
abstract：:Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.10.3255
更新日期：1983-05-25 00:00:00
abstract：:To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and micro-sequen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.15.2882
更新日期：2000-08-01 00:00:00
abstract：:RNA polymerase I-catalyzed synthesis of the Schizosaccharomyces pombe approximately 37S pre-rRNAs was shown to be sensitive to regulatory sequences located several kilobases upstream of the initiation site for the rRNA gene. An in vitro transcription system for RNA polymerase I-catalyzed RNA synthesis was established ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.3.659
更新日期：1997-02-01 00:00:00
abstract：:The synthesis of ribosomal precursor RNA in Novikoff hepatoma (N1S1) cells is very sensitive to cordycepin (3'-dA). The synthesis of hnRNA, however, is resistant to inhibition concentrations of 3'-dA that completely block the synthesis of 45S ribosomal RNA precursor. We have examined the RNA polymerases present in the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.2.325
更新日期：1976-02-01 00:00:00
abstract：:Circular DNA aptamers are powerful candidates for therapeutic applications given their dramatically enhanced biostability. Herein we report the first effort to evolve circular DNA aptamers that bind a human protein directly in serum, a complex biofluid. Targeting human thrombin, this strategy has led to the discovery ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa800
更新日期：2020-11-04 00:00:00
abstract：:A 200 bp sequence including a stretch of 54 base pairs of alternating guanosine and adenosine nucleotide residues [(dG-dA)27.(dT-dC)27] was cloned in the simian virus 40 (SV40) genome between the KpnI and HpaII sites. This sequence was discovered earlier as part of a region limiting the amplification of sequences adja...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.16.8077
更新日期：1988-08-25 00:00:00
abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku130
更新日期：2014-04-01 00:00:00
abstract：:IHF and HU are two heterodimeric nucleoid-associated proteins (NAP) that belong to the same protein family but interact differently with the DNA. IHF is a sequence-specific DNA-binding protein that bends the DNA by over 160°. HU is the most conserved NAP, which binds non-specifically to duplex DNA with a particular pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1236
更新日期：2012-04-01 00:00:00
abstract：:In the process of establishing more and more complete annotations of eukaryotic genomes, a constantly growing number of alternative splicing (AS) events has been reported over the last decade. Consequently, the increasing transcript coverage also revealed the real complexity of some variations in the exon-intron struc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm311
更新日期：2007-07-01 00:00:00
abstract：:The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.13.4453
更新日期：1983-07-11 00:00:00
abstract：:ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp496
更新日期：2009-08-01 00:00:00
abstract：:In many organisms (e.g., gram-positive eubacteria) Gin-tRNA is not formed by direct glutaminylation of tRNAGln but by a specific transamidation of Glu-tRNAGln. We wondered whether a similar transamidation pathway also operates in the formation of Asn-tRNA in these organisms. Therefore we tested in S-100 preparations o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.14.2648
更新日期：1996-07-15 00:00:00
abstract：:We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.10.3375
更新日期：1983-05-25 00:00:00
abstract：:Acinetobacter sp. strain ADP1 is a nutritionally versatile soil bacterium closely related to representatives of the well-characterized Pseudomonas aeruginosa and Pseudomonas putida. Unlike these bacteria, the Acinetobacter ADP1 is highly competent for natural transformation which affords extraordinary convenience for ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh910
更新日期：2004-10-28 00:00:00
abstract：:The proportion of lysine tRNA represented by the isoacceptor species lysine tRNA4 has previously been shown to be largest in cells with the greatest ability to proliferate. Using reverse phase chromatography (RPC-5), we have analyzed the changes in the relative quantities of lysine tRNA species which occur in differen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.3.851
更新日期：1978-03-01 00:00:00
abstract：:AvrBs3, the founding member of the Xanthomonas transcription-activator-like effectors (TALEs), is translocated into the plant cell where it localizes to the nucleus and acts as transcription factor. The DNA-binding domain of AvrBs3 consists of 17.5 nearly-identical 34 amino acid-repeats. Each repeat specifies binding ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku341
更新日期：2014-06-01 00:00:00
abstract：:The cold shock domain is one of the most highly conserved motifs between bacteria and higher eukaryotes. Y-box-binding proteins represent a subfamily of cold shock domain proteins with pleiotropic functions, ranging from transcription in the nucleus to translation in the cytoplasm. These proteins have been investigate...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1077
更新日期：2014-12-01 00:00:00
abstract：:The surface accessibility of macromolecules plays a key role in modulating molecular recognition events. RNA is a complex and dynamic molecule involved in many aspects of gene expression. However, there are few experimental methods available to measure the accessible surface of RNA. Here, we investigate the accessible...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1062
更新日期：2008-03-01 00:00:00
abstract：:Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq456
更新日期：2010-10-01 00:00:00
abstract：:Regulatory T-cells (Treg) play an essential role in the negative regulation of immune answers by developing an attenuated cytokine response that allows suppressing proliferation and effector function of T-cells (CD4(+) Th). The transcription factor FoxP3 is responsible for the regulation of many genes involved in the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr444
更新日期：2011-10-01 00:00:00
abstract：:The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and overexpressed it in E. coli. Upon induction with IPTG, M.MspI constitutes...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.7.1579
更新日期：1992-04-11 00:00:00
abstract：:Hepatitis C virus (HCV) translation initiation is directed by an internal ribosome entry site (IRES) and regulated by distant regions at the 3'-end of the viral genome. Through a combination of improved RNA chemical probing methods, SHAPE structural analysis and screening of RNA accessibility using antisense oligonucl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks927
更新日期：2012-12-01 00:00:00
