Chemical reactivity of matched cytosine and thymine bases near mismatched and unmatched bases in a heteroduplex between DNA strands with multiple differences.

abstract：:We analysed the genetic stability of two subtelomeric genes of the human malaria parasite Plasmodium falciparum. A PCR based assay, using a telomere and a target-gene specific primer was used to detect potential chromosome rearrangements. We show that chromosome breakage and the formation of new telomeres occur freque...

journal_title：Nucleic acids research

authors： Scherf A,Mattei D

更新日期：1992-04-11 00:00:00

abstract：:PrankWeb is an online resource providing an interface to P2Rank, a state-of-the-art method for ligand binding site prediction. P2Rank is a template-free machine learning method based on the prediction of local chemical neighborhood ligandability centered on points placed on a solvent-accessible protein surface. Points...

journal_title：Nucleic acids research

authors： Jendele L,Krivak R,Skoda P,Novotny M,Hoksza D

更新日期：2019-07-02 00:00:00

abstract：:The 279-bp major breakpoint region (mbr) within the 3'-untranslated region (3'-UTR) of the BCL2 gene is a binding site of special AT-rich sequence binding protein 1 (SATB1) that is well known to participate in the long-range regulation of gene transcription. Our previous studies have revealed that the mbr could regula...

journal_title：Nucleic acids research

authors： Gong F,Sun L,Wang Z,Shi J,Li W,Wang S,Han X,Sun Y

更新日期：2011-06-01 00:00:00

abstract：:The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers...

journal_title：Nucleic acids research

authors： Griffais R,André PM,Thibon M

更新日期：1991-07-25 00:00:00

abstract：:The cells of the bronchial epithelium of man are targets for benzo(a)pyrene carcinogenesis. When cultures of these cells, and of non-target fibroblasts, are exposed to [3H]-benzo(a)pyrene, we find that the epithelial cells metabolise and bind to DNA far greater amounts of benzpyrene than do fibroblasts. By analysis of...

journal_title：Nucleic acids research

authors： Arrand JE,Murray AM

更新日期：1982-03-11 00:00:00

abstract：:Heptadecaoligodeoxyribonucleotides containing one or more of the bases, 6H,8H-3,4-dihydropyrimido[4,5-c][1,2]oxazin-7-one (P), 2-amino-6-methoxyaminopurine (K), and hypoxanthine (I) and combinations of P with K and I have been synthesised on a DNA synthesiser. The stability of duplexes containing these basemodified ol...

journal_title：Nucleic acids research

authors： Lin PK,Brown DM

更新日期：1992-10-11 00:00:00

abstract：:The cis-requirements for the first editing site in the atp9 mRNA from pea mitochondria were investigated in an in vitro RNA editing system. Template RNAs deleted 5' of -20 are edited correctly, but with decreased efficiency. Deletions between -20 and the edited nucleotide abolish editing activity. Substitution of the ...

journal_title：Nucleic acids research

authors： Takenaka M,Neuwirt J,Brennicke A

更新日期：2004-08-04 00:00:00

abstract：:The RNA sequences boxA, boxB and boxC constitute the nut regions of phage lambda. They nucleate the formation of a termination-resistant RNA polymerase complex on the lambda chromosome. The complex includes E. coli proteins NusA, NusB, NusG and NusE, and the lambda N protein. A complex that includes the Nus proteins a...

journal_title：Nucleic acids research

authors： Prasch S,Jurk M,Washburn RS,Gottesman ME,Wöhrl BM,Rösch P

更新日期：2009-08-01 00:00:00

abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...

journal_title：Nucleic acids research

authors： Hartig A,Simon MM,Schuster T,Daugherty JR,Yoo HS,Cooper TG

更新日期：1992-11-11 00:00:00

abstract：:Tandem repeats in DNA have been under intensive study for many years, first, as a consequence of their usefulness as genomic markers and DNA fingerprints and more recently as their role in human disease and regulatory processes has become apparent. The Tandem Repeats Database (TRDB) is a public repository of informati...

journal_title：Nucleic acids research

authors： Gelfand Y,Rodriguez A,Benson G

更新日期：2007-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are short, non-protein coding RNAs that direct the widespread phenomenon of post-transcriptional regulation of metazoan genes. The mature approximately 22-nt long RNA molecules are processed from genome-encoded stem-loop structured precursor genes. Hundreds of such genes have been experimentally val...

journal_title：Nucleic acids research

authors： Gerlach D,Kriventseva EV,Rahman N,Vejnar CE,Zdobnov EM

更新日期：2009-01-01 00:00:00

abstract：:We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S defic...

journal_title：Nucleic acids research

authors： Beach LR,Spencer D,Randall PJ,Higgins TJ

更新日期：1985-02-11 00:00:00

abstract：:8-Oxo-7,8-dihydroguanine (8-oxoGua) is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The MutT protein of Escherichia col...

journal_title：Nucleic acids research

authors： Ishibashi T,Hayakawa H,Ito R,Miyazawa M,Yamagata Y,Sekiguchi M

更新日期：2005-07-07 00:00:00

abstract：:The cartilage matrix is composed of characteristic components including type II collagen, aggrecan and link protein. In this paper, we report two DNA elements that regulate the link protein gene. Using transient transfection assays with link protein gene constructs in chondrocytes, chloramphenicol acetyl transferase (...

journal_title：Nucleic acids research

authors： Rhodes C,Yamada Y

更新日期：1995-06-25 00:00:00

abstract：:eggNOG is a public database of orthology relationships, gene evolutionary histories and functional annotations. Here, we present version 5.0, featuring a major update of the underlying genome sets, which have been expanded to 4445 representative bacteria and 168 archaea derived from 25 038 genomes, as well as 477 euka...

journal_title：Nucleic acids research

authors： Huerta-Cepas J,Szklarczyk D,Heller D,Hernández-Plaza A,Forslund SK,Cook H,Mende DR,Letunic I,Rattei T,Jensen LJ,von Mering C,Bork P

更新日期：2019-01-08 00:00:00

abstract：:RNA interference represents a potent intervention for cancer treatment but requires a robust delivery agent for transporting gene-modulating molecules, such as small interfering RNAs (siRNAs). Although numerous molecular approaches for siRNA delivery are adequate in vitro, delivery to therapeutic targets in vivo is li...

journal_title：Nucleic acids research

authors： Alonso-Valenteen F,Pacheco S,Srinivas D,Rentsendorj A,Chu D,Lubow J,Sims J,Miao T,Mikhael S,Hwang JY,Abrol R,Medina Kauwe LK

更新日期：2019-12-02 00:00:00

abstract：:ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F(0)F(...

journal_title：Nucleic acids research

authors： Burger G,Lang BF,Braun HP,Marx S

更新日期：2003-05-01 00:00:00

abstract：:Expression of a proof-reading deficient form of mitochondrial DNA (mtDNA) polymerase gamma, POLG, causes early death accompanied by features of premature ageing in mouse. However, the mechanism of cellular senescence remains unresolved. In addition to high levels of point mutations of mtDNA, the POLG mutator mouse har...

journal_title：Nucleic acids research

authors： Bailey LJ,Cluett TJ,Reyes A,Prolla TA,Poulton J,Leeuwenburgh C,Holt IJ

更新日期：2009-04-01 00:00:00

abstract：:PubChem (http://pubchem.ncbi.nlm.nih.gov) is a public repository for biological activity data of small molecules and RNAi reagents. The mission of PubChem is to deliver free and easy access to all deposited data, and to provide intuitive data analysis tools. The PubChem BioAssay database currently contains 500,000 des...

journal_title：Nucleic acids research

authors： Wang Y,Xiao J,Suzek TO,Zhang J,Wang J,Zhou Z,Han L,Karapetyan K,Dracheva S,Shoemaker BA,Bolton E,Gindulyte A,Bryant SH

更新日期：2012-01-01 00:00:00

abstract：:Analysis of the human VK (ref. 2) gene locus led to the detection of a new sequence family (L sequences). Its copy number is in the range of 10(2). The L sequences, which are about 500 bp long, are found as part of the 3' flanking regions of a clustered set of human VKI genes but they occur also separate from the gene...

journal_title：Nucleic acids research

authors： Straubinger B,Pech M,Mühlebach K,Jaenichen HR,Bauer HG,Zachau HG

更新日期：1984-07-11 00:00:00

abstract：:Electrospray mass spectrometry was evaluated regarding the reliability of the determination of the stoichiometries and equilibrium association constants from single spectra. Complexes between minor groove binders (Hoechst 33258, Hoechst 33342, DAPI, netropsin and berenil) and 12mer oligonucleotide duplexes with a cent...

journal_title：Nucleic acids research

authors： Rosu F,Gabelica V,Houssier C,De Pauw E

更新日期：2002-08-15 00:00:00

abstract：:The genome of Kirsten murine sarcoma virus was formed by recombination between Kirsten murine leukaemia virus sequences, and rat sequences derived from a retrovirus-like '30S' (VL30) genetic element encompassing the Kras oncogene. Using cloned DNAs we have determined the nucleotide sequences of the long terminal repea...

journal_title：Nucleic acids research

authors： Norton JD,Connor J,Avery RJ

更新日期：1984-09-11 00:00:00

abstract：:Here we examined the role of cellular vitamin C in genotoxicity of carcinogenic chromium(VI) that requires reduction to induce DNA damage. In the presence of ascorbate (Asc), low 0.2-2 microM doses of Cr(VI) caused 10-15 times more chromosomal breakage in primary human bronchial epithelial cells or lung fibroblasts. D...

journal_title：Nucleic acids research

authors： Reynolds M,Stoddard L,Bespalov I,Zhitkovich A

更新日期：2007-01-01 00:00:00

abstract：:We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that...

journal_title：Nucleic acids research

authors： Colantuoni V,Romano V,Bensi G,Santoro C,Costanzo F,Raugei G,Cortese R

更新日期：1983-11-25 00:00:00

abstract：:The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA ...

journal_title：Nucleic acids research

authors： Rafalski A,Kohli J,Agris P,Söll D

更新日期：1979-06-25 00:00:00

abstract：:The mammalian transcription factor SRF and the yeast regulatory protein MCM1 contain DNA binding domains that are 70% identical; moreover, both proteins can bind the serum response element in the human c-fos promoter. Here we present an analysis of MCM1 sequence specificity by selection of sites from random sequence o...

journal_title：Nucleic acids research

authors： Wynne J,Treisman R

更新日期：1992-07-11 00:00:00

abstract：:In squid (Loligo bleekeri) mitochondria, the two 3'-terminal nucleotides (G72-G73) of the tRNA(Tyr) gene overlap with the two 5'-terminal nucleotides (G1-G2) of the downstream tRNA(Cys) gene. To elucidate the processing mechanism(s) of the tRNA molecules derived from this region, tRNAs were analyzed by sequencing cDNA...

journal_title：Nucleic acids research

authors： Tomita K,Ueda T,Watanabe K

更新日期：1996-12-15 00:00:00

abstract：:DNA primases recognize single-stranded DNA (ssDNA) sequences to synthesize RNA primers during lagging-strand replication. Arabidopsis thaliana encodes an ortholog of the DNA primase-helicase from bacteriophage T7, dubbed AtTwinkle, that localizes in chloroplasts and mitochondria. Herein, we report that AtTwinkle synth...

journal_title：Nucleic acids research

authors： Peralta-Castro A,Baruch-Torres N,Brieba LG

更新日期：2017-10-13 00:00:00

abstract：:USF is a helix-loop-helix transcription factor that, like Myc, recognizes the DNA binding motif CACGTG. Two different forms of USF, characterized by apparent molecular weights of 43,000 and 44,000, were originally identified in HeLa cells by biochemical analysis. Clones for the 43-kDa USF were first characterized, but...

journal_title：Nucleic acids research

authors： Sirito M,Lin Q,Maity T,Sawadogo M

更新日期：1994-02-11 00:00:00

abstract：:The solubility of adult beta-globin chromatin (beta A chromatin) from immature chicken red blood cells can be controlled by the presence or absence of n-butyrate in a cell incubation medium. In the absence of n-butyrate, only a small percentage (approximately 4%) of the total beta A chromatin is in a soluble chromatin...

journal_title：Nucleic acids research

authors： Ferenz CR,Nelson DA

更新日期：1985-03-25 00:00:00