Abstract:
:The circular dichroism spectra of Hg(II) complexes with native calf thymus DNA, chemically methylated Streptomyces chrysomallus DNA and with Ag(I)-DNA complexes were measured in the region of 220 - 340 nm. As a main result a conversion of the conservative CD spectrum of DNA to a distinct nonconservative type of CD spectrum for the complexes occurs with increasing Hg(II) concentration. The CD spectra of the Hg(II) complexes as well as some additional arguments strongly support the idea, that DNA in the complex undergoes a structural transition to a more condensed state with 4 -like character.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Walter A,Luck Gdoi
10.1093/nar/4.3.539subject
Has Abstractpub_date
1977-03-01 00:00:00pages
539-50issue
3eissn
0305-1048issn
1362-4962journal_volume
4pub_type
杂志文章abstract::REPPER (REPeats and their PERiodicities) is an integrated server that detects and analyzes regions with short gapless repeats in protein sequences or alignments. It finds periodicities by Fourier Transform (FTwin) and internal similarity analysis (REPwin). FTwin assigns numerical values to amino acids that reflect cer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki405
更新日期:2005-07-01 00:00:00
abstract::A minizyme is a hammerhead ribozyme with short oligonucleotide linkers instead of stem-loop II. In a previous study we demonstrated that a minizyme with high activity forms a dimeric structure with a common stem II. Because of their dimeric structure, minizymes are potentially capable of cleaving a substrate at two di...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.12.2302
更新日期:1996-06-15 00:00:00
abstract::Recently published alignments of available 5 S rRNA sequences have shown that a rigid base pairing pattern, pointing to the existence of a universal five-helix secondary structure for all 5 S RNAs, can be superimposed on such alignments. For a few species, the alignment and the base pairing pattern show distortions wi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.5.1245
更新日期:1983-03-11 00:00:00
abstract::The human lymphoid cell lines Walker and Daudi constitute a particularly suitable system for studies on the chromatin structure of K light chain genes (see preceding paper). The rearranged and non-rearranged alleles of Walker cells were found to be about equally sensitive towards digestion with DNAase I. A DNAase I hy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.18.7007
更新日期:1984-09-25 00:00:00
abstract::The ribosomal frameshifting signal of the mouse embryonal carcinoma differentiation regulated (Edr) gene represents the sole documented example of programmed -1 frameshifting in mammalian cellular genes [Shigemoto,K., Brennan,J., Walls,E,. Watson,C.J., Stott,D., Rigby,P.W. and Reith,A.D. (2001), Nucleic Acids Res., 29...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki299
更新日期:2005-03-14 00:00:00
abstract::During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluorescence signal emitted ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky740
更新日期:2018-12-14 00:00:00
abstract::The NONCODE database is an integrated knowledge database designed for the analysis of non-coding RNAs (ncRNAs). Since NONCODE was first released 3 years ago, the number of known ncRNAs has grown rapidly, and there is growing recognition that ncRNAs play important regulatory roles in most organisms. In the updated vers...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1011
更新日期:2008-01-01 00:00:00
abstract::The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.23.6527
更新日期:1991-12-11 00:00:00
abstract::Most RNA molecules collapse rapidly and reach the native state through a pathway that contains numerous traps and unproductive intermediates. The D135 group II intron ribozyme is unusual in that it can fold slowly and directly to the native state, despite its large size and structural complexity. Here we use hydroxyl ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki973
更新日期:2005-11-27 00:00:00
abstract::Partial restriction digestion is used to map restriction sites and the location of genes within yeast artificial chromosomes (YACs). Locus-specific probes are hybridised to the partially digested YAC DNA and the fragments to which they hybridise are compared with the pattern of partial digestion products that include ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.22.4495
更新日期:1996-11-15 00:00:00
abstract::I-motif DNAs have been widely employed as robust modulating components to construct reconfigurable DNA nanodevices that function well in acidic cellular environments. However, they generally display poor interactivity with fluorescent ligands under these complex conditions, illustrating a major difficulty in utilizing...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa020
更新日期:2020-02-28 00:00:00
abstract::JSNP is a repository of Japanese Single Nucleotide Polymorphism (SNP) data, begun in 2000 and developed through the Prime Minister's Millennium Project. The aim of this undertaking is to identify and collate up to 150 000 SNPs from the Japanese population, located in genes or in adjacent regions that might influence t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.1.158
更新日期:2002-01-01 00:00:00
abstract::Activating sequence factor 1 (ASF-1) is a nuclear DNA-binding activity that is found in monocots and dicots. It interacts with several TGACG-containing elements that have been characterized from viral and T-DNA genes, the prototypes of which are the as-1 element of the CaMV 35S promoter and the ocs element from the oc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.18.3778
更新日期:1995-09-25 00:00:00
abstract::The TWIST1 bHLH transcription factor controls embryonic development and cancer processes. Although molecular and genetic analyses have provided a wealth of data on the role of bHLH transcription factors, very little is known on the molecular mechanisms underlying their binding affinity to the E-box sequence of the pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw334
更新日期:2016-06-20 00:00:00
abstract::High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed. In this work, we have developed and implemented a set of synthetic 16S rRNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw984
更新日期:2017-02-28 00:00:00
abstract::The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 am...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.2.307
更新日期:1991-01-25 00:00:00
abstract::The 5'-cap of spliceosomal small nuclear RNAs, some small nucleolar RNAs and of telomerase RNA was found to be hypermethylated in vivo. The Trimethylguanosine Synthase 1 (TGS1) mediates this conversion of the 7-methylguanosine-cap to the 2,2,7-trimethylguanosine (m(3)G)-cap during maturation of the RNPs. For mammalian...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp249
更新日期:2009-07-01 00:00:00
abstract::We have developed efficient methods for creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of DNA mapping and sequencing. A novel plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial tran...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.18.3765
更新日期:1994-09-11 00:00:00
abstract::Sexual differentiation in Drosophila is regulated through alternative splicing of doublesex. Female-specific splicing is activated through the activity of splicing enhancer complexes assembled on multiple repeat elements. Each of these repeats serves as a binding platform for the cooperative assembly of a heterotrimer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl984
更新日期:2006-01-01 00:00:00
abstract::To unravel the network of transcription factors established during development it is important to understand how genes specifically expressed during embryogenesis are regulated. Oct-4 is a transcription factor whose expression is associated with an undifferentiated cell phenotype in the early mouse embryo and is downr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.6.901
更新日期:1994-03-25 00:00:00
abstract::The active form of Escherichia coli DNA polymerase V responsible for damage-induced mutagenesis is a multiprotein complex (UmuD'(2)C-RecA-ATP), called pol V Mut. Optimal activity of pol V Mut in vitro is observed on an SSB-coated single-stranded circular DNA template in the presence of the β/γ complex and a transactiv...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks233
更新日期:2012-07-01 00:00:00
abstract::Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGC...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.10.4607
更新日期:1988-05-25 00:00:00
abstract::Interactions between proteins bound to distant sites along a DNA molecule require bending and twisting deformations in the intervening DNA. In certain systems, the sterically allowed protein-DNA and protein-protein interactions are hypothesized to produce loops with distinct geometries that may also be thermodynamical...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn389
更新日期:2008-07-01 00:00:00
abstract::Here we compare several methods for predicting oligonucleotide frequencies in 691 kb of Drosophila melanogaster DNA. As in previous work on Escherichia coli and Saccharomyces cerevisiae, a relatively simple equation based on tetranucleotide frequencies can be used in predicting frequencies of higher order oligonucleot...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.14.3651
更新日期:1992-07-25 00:00:00
abstract::The study of bacterial isolates or communities requires the analysis of the therein included plasmids in order to provide an extensive characterization of the organisms. Plasmids harboring resistance and virulence factors are of especial interest as they contribute to the dissemination of antibiotic resistance. As the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1050
更新日期:2019-01-08 00:00:00
abstract::The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide sequences of the promoter regions determined. A mutation within the trfA promoter sequence is associated with loss of kilD act...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.8.3619
更新日期:1984-04-25 00:00:00
abstract::Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.23.4983
更新日期:1994-11-25 00:00:00
abstract::The sequence requirements for specific and efficient transcription from the 16S/23S rRNA promoter of Sulfolobus shibatae were analysed by point mutations and by cassette mutations using an in vitro transcription system. The examination of the box A-containing distal promoter element (DPE) showed the great importance o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.20.5423
更新日期:1992-10-25 00:00:00
abstract::A fraction of the ribosomal 28S genes in some insects are interrupted at specific sites by insertion elements R1 and R2 (also called Type I and II). These elements contain long open-reading frames with homology to reverse transcriptase. We have identified in the silkmoth, Bombyx mori, copies of these elements which ha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.22.10561
更新日期:1988-11-25 00:00:00
abstract::The Transcription Regulatory Regions Database (TRRD) is a curated database designed for accumulation of experimental data on extended regulatory regions of eukaryotic genes, the regulatory elements they contain, i.e., transcription factor binding sites, promoters, enhancers, silencers, etc., and expression patterns of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.1.303
更新日期:1999-01-01 00:00:00