Abstract：
:Sexual differentiation in Drosophila is regulated through alternative splicing of doublesex. Female-specific splicing is activated through the activity of splicing enhancer complexes assembled on multiple repeat elements. Each of these repeats serves as a binding platform for the cooperative assembly of a heterotrimeric complex consisting of the SR proteins Tra, Tra2 and 9G8. Using quantitative kinetic analyses, we demonstrate that each component of the enhancer complex is capable of recruiting the spliceosome. Surprisingly, Tra, Tra2 and 9G8 are much stronger splicing activators than other SR protein family members and their activation potential is significantly higher than expected from their serine/arginine content. 9G8 activates splicing not only through its RS domains but also through its RNA-binding domain. The RS domains of Tra and Tra2 are required but not sufficient for efficient complex assembly. Thus, the regulated assembly of the dsx enhancer complexes leads to the generation of an extended activation domain to guarantee the 'all or none' splicing switch that is required during Drosophila sexual differentiation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sciabica KS,Hertel KJdoi
10.1093/nar/gkl984subject
Has Abstractpub_date
2006-01-01 00:00:00pages
6612-20issue
22eissn
0305-1048issn
1362-4962pii
gkl984journal_volume
34pub_type
杂志文章abstract：:Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with 13C and 15N. Imino proton signals were assigned by analysis...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.3.766
更新日期：2002-02-01 00:00:00
abstract：:Ribonucleotides are misincorporated into replicating DNA due to the similarity of deoxyribonucleotides and ribonucleotides, the high concentration of ribonucleotides in the nucleus and the imperfect accuracy of replicative DNA polymerases in choosing the base with the correct sugar. Embedded ribonucleotides change cer...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gku773
更新日期：2014-01-01 00:00:00
abstract：:Approximately 30% of human tumors characterized to date express DNA polymerase beta (pol beta) variant proteins. Two of the polymerase beta cancer-associated variants are sequence-specific mutators, and one of them binds to DNA but has no polymerase activity. The Leu22Pro (L22P) DNA polymerase beta variant was identif...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1053
更新日期：2008-02-01 00:00:00
abstract：:VapD-like virulence-associated proteins have been found in many organisms, but little is known about this protein family including the 3D structure of these proteins. Recently, a relationship between the Cas2 family of ribonucleases associated with the CRISPR system of microbial immunity and VapD was suggested. Here, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1305
更新日期：2012-05-01 00:00:00
abstract：:During culture, a chicken B cell line DT40 spontaneously mutates immunoglobulin (Ig) genes by gene conversion, which involves activation-induced cytidine deaminase (AID)-dependent homologous recombination of the variable (V) region gene with upstream pseudo-V genes. To explore whether this mutation mechanism can targe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnj013
更新日期：2006-01-18 00:00:00
abstract：:In this article, we introduce the Gypsy Database (GyDB) of mobile genetic elements, an in-progress database devoted to the non-redundant analysis and evolutionary-based classification of mobile genetic elements. In this first version, we contemplate eukaryotic Ty3/Gypsy and Retroviridae long terminal repeats (LTR) ret...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm697
更新日期：2008-01-01 00:00:00
abstract：:More and more evidences demonstrate that the long non-coding RNAs (lncRNAs) play many key roles in diverse biological processes. There is a critical need to annotate the functions of increasing available lncRNAs. In this article, we try to apply a global network-based strategy to tackle this issue for the first time. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks967
更新日期：2013-01-01 00:00:00
abstract：:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.13.6027
更新日期：1988-07-11 00:00:00
abstract：:The laboratory mouse is the premier animal model for studying human biology because all life stages can be accessed experimentally, a completely sequenced reference genome is publicly available and there exists a myriad of genomic tools for comparative and experimental research. In the current era of genome scale, dat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1115
更新日期：2013-01-01 00:00:00
abstract：:SIRT6 is critical for activating transcription of Nuclear factor (erythroid-derived 2)-like 2 (NRF2) responsive genes during oxidative stress. However, while the mechanism of SIRT6-mediated silencing is well understood, the mechanism of SIRT6-mediated transcriptional activation is unknown. Here, we employed SIRT6 sepa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz528
更新日期：2019-09-05 00:00:00
abstract：:The three CD3 genes on human chromosome 11q23 encode proteins (gamma, delta and epsilon) which form part of the antigen receptor on T lymphocytes. All three genes are clustered within 50 kb and are activated approximately contemporaneously during the early stages of T cell ontogeny. In order to pinpoint potential regu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.3.459
更新日期：1990-02-11 00:00:00
abstract：:Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.3027
更新日期：1991-06-11 00:00:00
abstract：:We report the first investigation of oligoribonucleotides containing a few 1-(2-deoxy-2-alpha-C-hydroxymethyl-beta-D-ribofuranosyl)thymine units (or 2'-hm-dT, abbreviated in this work as 'H'). Both the 2'-CH2O-phosphoramidite and 3'-O-phosphoramidite derivatives of H were synthesized and incorporated into both 2',5'-R...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki1003
更新日期：2005-12-23 00:00:00
abstract：:Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have developed a low copy number cosmid vector based on the E. coli F factor replicon (Fosmid). We have tested relative stability o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.5.1083
更新日期：1992-03-11 00:00:00
abstract：:Loss-of-function in melanocortin 1 receptor (MC1R), a GS protein-coupled receptor that regulates signal transduction through cAMP and protein kinase A (PKA) in melanocytes, is a major inherited melanoma risk factor. Herein, we report a novel cAMP-mediated response for sensing and responding to UV-induced DNA damage re...
journal_title：Nucleic acids research
pub_type： 杂志文章,收录出版
doi：10.1093/nar/gkw871
更新日期：2016-12-15 00:00:00
abstract：:We have cloned and sequenced the lasR gene, which is involved in the transcriptional activation of several pathogenic factors, from Pseudomonas aeruginosa IFO3455 and PA103. These clones were predicted to be an open reading frame of 239 amino acids as reported for the PAO1 strain. There is only a single base change re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.18.3706
更新日期：1994-09-11 00:00:00
abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp853
更新日期：2010-01-01 00:00:00
abstract：:Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777. Furthermore, a specific retarded band is observed when electrophoretic mobility shift ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm782
更新日期：2007-01-01 00:00:00
abstract：:Proteins perform biological functions through cascading interactions with each other by forming protein complexes. As a result, interactions among proteins, called protein-protein interactions (PPIs) are not completely free from selection constraint during evolution. Therefore, the identification and analysis of PPI c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky378
更新日期：2018-07-02 00:00:00
abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw580
更新日期：2016-09-30 00:00:00
abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. PHF1 [plant homeodomain (PHD) finger protein 1], which contains two kinds of histone reader modules, a Tudor domain and two PHD fin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky461
更新日期：2018-07-27 00:00:00
abstract：:DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-ba...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.6.1144
更新日期：1996-03-15 00:00:00
abstract：:The NCBI has introduced two new web resources-LocusLink and RefSeq-that facilitate retrieval of gene-based information and provide reference sequence standards. These resources are designed to provide a non-redundant view of current knowledge about human genes, transcripts and proteins. Additional information about th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.126
更新日期：2000-01-01 00:00:00
abstract：:Primer design is a fundamental technique that is widely used for polymerase chain reaction (PCR). Although many methods have been proposed for primer design, they require a great deal of manual effort to generate feasible and valid primers, including homology tests on off-target sequences using BLAST-like tools. That ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv632
更新日期：2015-11-16 00:00:00
abstract：:The Intronerator (http://www.cse.ucsc.edu/ approximately kent/intronerator/ ) is a set of web-based tools for exploring RNA splicing and gene structure in Caenorhabditis elegans. It includes a display of cDNA alignments with the genomic sequence, a catalog of alternatively spliced genes and a database of introns. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.91
更新日期：2000-01-01 00:00:00
abstract：:The current version of PLMItRNA has been realized to constitute a database for tRNA molecules and genes identified in the mitochondria of all green plants ( Viridiplantae ). It is the enlargement of a previous database originally restricted to seed plants [Ceci,L.R., Volpicella,M., Liuni,S., Volpetti,V., Licciulli,F. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.159
更新日期：2000-01-01 00:00:00
abstract：:The -175 T greater than C mutation in the promoter of the A gamma- or G gamma-globin gene causes a 50-100 fold increase of the expression of the respective gene in adult erythroid cells (Hereditary Persistence of Fetal Hemoglobin). We show here that this mutation increases 3-9 fold the expression of a gamma-CAT report...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.14.5509
更新日期：1989-07-25 00:00:00
abstract：:The nucleotide sequence of the chick beta-actin gene was determined. The gene contains 5 introns; 4 interrupt the translated region at codons 41/42, 120/122, 267, 327/328 and a large intron occurs in the 5' untranslated region. The gene has a 97 nucleotide 5'-untranslated region and a 594 nucleotide 3'-untranslated re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.23.8287
更新日期：1983-12-10 00:00:00
abstract：:Human minisatellite probes cross-hybridize to mouse DNA and detect multiple variable loci. The resulting DNA "fingerprints" vary substantially between inbred strains but relatively little within an inbred strain. By studying the segregation of variable DNA fragments in BXD recombinant inbred strains of mice, at least ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.7.2823
更新日期：1987-04-10 00:00:00
abstract：:The Mouse Genome Database (MGD) (http://www.informatics.jax.org) one component of a community database resource for the laboratory mouse, a key model organism for interpreting the human genome and for understanding human biology. MGD strives to provide an extensively integrated information resource with experimental d...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg047
更新日期：2003-01-01 00:00:00
