Abstract:
:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double and triple digestions. Loci for 2 ribosomal RNA operons and 2 tRNA operons have been determined by probing with cloned genes and the broad regions of the replication origin and terminus have also been outlined by in vivo labelling studies.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Pyle LE,Finch LRdoi
10.1093/nar/16.13.6027subject
Has Abstractpub_date
1988-07-11 00:00:00pages
6027-39issue
13eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::The interaction of N--Acetyl--Phe--tRNA Phe with 70 S ribosomes is a reversible process in the absence as well as in the presence of messenger. The equilibrium binding constants of these interactions were measured at different magnesium concentrations and temperatures and thermodynamical quantities computed. The entha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3871
更新日期:1978-10-01 00:00:00
abstract::Deproteinated 16S RNA was iodinated at pH 5.0 in an aqueous solution containing TlCl3 plus KI for 1-5 hours at 42 degrees C. Under these conditions 33 moles of iodine are incorporated per mole of RNA. As judged by sucrose gradient sedimentation, the iodinated RNA does not exhibit any large alteration in conformation a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.11.3001
更新日期:1976-11-01 00:00:00
abstract::Myotonic dystrophy type 1 (DM1) is caused by the expansion of a (CTG).(CAG) repeat in the DMPK gene on chromosome 19q13.3. At least 17 neurological diseases have similar genetic mutations, the expansion of DNA repeats. In most of these disorders, the disease severity is related to the length of the repeat expansion, a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh976
更新日期:2004-12-01 00:00:00
abstract::Pig Genomic Information System (PigGIS) is a web-based depository of pig (Sus scrofa) genomic learning mainly engineered for biomedical research to locate pig genes from their human homologs and position single nucleotide polymorphisms (SNPs) in different pig populations. It utilizes a variety of sequence data, includ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl808
更新日期:2007-01-01 00:00:00
abstract::We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.22.7769
更新日期:1983-11-25 00:00:00
abstract::G-quadruplexes (G4s) are nucleic acid structure motifs that are of significance in chemistry and biology. The function of G4s is often governed by their interaction with G4-binding proteins. Few categories of G4-specific tools have been developed to inhibit G4-protein interactions; however, until now there is no aptam...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa759
更新日期:2020-10-09 00:00:00
abstract::Vaccinia virus DNA topoisomerase catalyzes resolution of synthetic Holliday junctions in vitro. The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/, that are opposed within a partially mobile four-way junction. Efficient resolution occurs on a junction with a 10 bp segment of branc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.14.2658
更新日期:2000-07-15 00:00:00
abstract::EpoDB is a database of genes expressed in vertebrate red blood cells. It is also a prototype for the creation of cell and tissue-specific databases from multiple external sources. The information in EpoDB obtained from GenBank, SWISS-PROT, Transfac, TRRD and GERD is curated to provide high quality data for sequence an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.1.200
更新日期:1999-01-01 00:00:00
abstract::We previously showed that histone H4 serine-1 phosphorylation (H4S1ph) is evolutionarily conserved during gametogenesis, and contributes to post-meiotic nuclear compaction and to full completion of sporulation in the yeast Saccharomyces cerevisiae. Previous studies showed that H4S1ph and another modification of the sa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq218
更新日期:2010-08-01 00:00:00
abstract::Structured RNA molecules form complex 3D architectures stabilized by multiple interactions involving the nucleotide base, sugar and phosphate moieties. A significant percentage of the bases in structured RNA molecules in the Protein Data Bank (PDB) hydrogen-bond with phosphates of other nucleotides. By extracting and ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp468
更新日期:2009-08-01 00:00:00
abstract::The nucleotide sequence of Drosophila melanogaster methionine tRNAi was determined to be: pA-G-C-A-G-A-G-U-m1G-m2G-C-G-C-A-G-U-G-G-A-A-G-C-G-U-m2G-C-U-G-G-G-C-C-C-A-U-t6A-A-C-C-C-A-G-A-G-m7G-D-m5C-C-C-G-A-G-G-A-U-C-G-m1A-A-A-C-C-U-U-G-C-U-C-U-G-C-U-A-C-C-A(OH). It differs from vertebrate initiator tRNAs in only 6 out ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.2.421
更新日期:1979-02-01 00:00:00
abstract::A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.13.4409
更新日期:1983-07-11 00:00:00
abstract::The NusA protein is a universally conserved bacterial transcription elongation factor that binds RNA polymerase (RNAP). When functioning independently, NusA enhances intrinsic termination. Paradoxically, NusA stimulates the function of the N and Q antiterminator proteins of bacteriophage λ. The mechanistic basis for N...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw198
更新日期:2016-06-20 00:00:00
abstract::The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 am...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.21.7607
更新日期:1985-11-11 00:00:00
abstract::We have prepared several novel phosphoramidites and have synthesised oligonucleotides incorporating them internally. The presence of these residues in an oligonucleotide template presents an impossible barrier to primed synthesis by Taq DNA polymerase. When extended as polymerase chain reaction products, these oligonu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.5.1155
更新日期:1993-03-11 00:00:00
abstract::alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3873
更新日期:1996-10-01 00:00:00
abstract::Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq801
更新日期:2011-01-01 00:00:00
abstract::Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIα interacts with specific RNA polymerases and chromatin-remodeling complexes...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt707
更新日期:2013-11-01 00:00:00
abstract::All exchangeable protons in a short DNA helix, d(CG)3 sodium salt, have been studied by proton nuclear magnetic resonance. The cytidine and guanosine amino protons have been assigned for the first time. As a function of temperature the cytidine amino protons and the imino protons behave very similarly, their relaxatio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.21.8269
更新日期:1984-11-12 00:00:00
abstract::Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw077
更新日期:2016-04-20 00:00:00
abstract::Impaired DNA damage repair, especially deficient transcription-coupled nucleotide excision repair, leads to segmental progeroid syndromes in human patients as well as in rodent models. Furthermore, DNA double-strand break signalling has been pinpointed as a key inducer of cellular senescence. Several recent findings s...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkm1065
更新日期:2007-01-01 00:00:00
abstract::Primer design is a fundamental technique that is widely used for polymerase chain reaction (PCR). Although many methods have been proposed for primer design, they require a great deal of manual effort to generate feasible and valid primers, including homology tests on off-target sequences using BLAST-like tools. That ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv632
更新日期:2015-11-16 00:00:00
abstract::It is a central issue to elucidate the new type of molecular recognition accompanied by a global structural change of a molecule upon binding to its targets. Here we investigate the driving force for the binding of R12 (a ribonucleic acid aptamer) and P16 (a partial peptide of a prion protein) during which P16 exhibit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku382
更新日期:2014-06-01 00:00:00
abstract::8-Oxo-7,8-dihydroguanine (8-oxoGua) is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The MutT protein of Escherichia col...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki682
更新日期:2005-07-07 00:00:00
abstract::We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchange chromatography. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.8.1300
更新日期:1995-04-25 00:00:00
abstract::A 9.2 kb segment of the maxi-circle of Trypanosoma brucei mitochondrial DNA contains the genes for cytochrome c oxidase subunits I and II (coxI and coxII) and seven Unassigned Reading Frames ("URFs"). The genes for coxI and coxII display considerable homology at the aminoacid level (38 and 25%, respectively) to the co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.19.7327
更新日期:1984-10-11 00:00:00
abstract::Recently, we reported a multicopy mammalian plasmid with a structure related to polyoma. The plasmid, named L factor, was found at a high copy number (5,000 or more per cell) in a subclone derived from mouse L cells. We attempted to utilize L factor as a plasmid vector for mammalian cells. A series of composite DNA co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.21.8815
更新日期:1987-11-11 00:00:00
abstract::The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn112
更新日期:2008-05-01 00:00:00
abstract::The vertebrate genome is a mosaic of regions differing dramatically in their G + C content. Those regions with a high G + C content contain the expected number of CpG dinucleotides and we propose that following methylation these have been protected from deamination by the increased stability of the surrounding DNA dup...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.14.5869
更新日期:1984-07-25 00:00:00
abstract::The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.13.4453
更新日期:1983-07-11 00:00:00