Contributions of DNA interstrand cross-links to aging of cells and organisms.

abstract：:Myocyte enhancer binding factor 2 (MEF2) proteins belong to the MADS box family of transcription factors and four MEF2 proteins, MEF2A, MEF2B, MEF2C and MEF2D, have been found. MEF2 proteins have been shown to play critical roles in differentiation of muscles and neuronal tissues. How transactivational activity of MEF...

journal_title：Nucleic acids research

authors： Choi SJ,Park SY,Han TH

更新日期：2001-07-01 00:00:00

abstract：:We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA molecules in nanofluidi...

journal_title：Nucleic acids research

authors： Nilsson AN,Emilsson G,Nyberg LK,Noble C,Stadler LS,Fritzsche J,Moore ER,Tegenfeldt JO,Ambjörnsson T,Westerlund F

更新日期：2014-09-01 00:00:00

abstract：:IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specific DNA binding to t...

journal_title：Nucleic acids research

authors： Shi L,Qiu D,Zhao G,Corthesy B,Lees-Miller S,Reeves WH,Kao PN

更新日期：2007-01-01 00:00:00

abstract：:The non-essential MGS1 gene of Saccharomyces cerevisiae is highly conserved in eukaryotes and encodes an enzyme containing both DNA-dependent ATPase and DNA annealing activities. MGS1 appears to function in post-replicational repair processes that contribute to genome stability. In this study, we identified MGS1 as a ...

journal_title：Nucleic acids research

authors： Kim JH,Kang YH,Kang HJ,Kim DH,Ryu GH,Kang MJ,Seo YS

更新日期：2005-10-26 00:00:00

abstract：:Monocrotaline is a pyrrolizidine alkaloid known to cause toxicity in humans and animals. Its mechanism of biological action is still unclear although DNA crosslinking has been suggested to a play a role in its activity. In this study we found that an active metabolite of monocrotaline, dehydromonocrotaline (DHM), alky...

journal_title：Nucleic acids research

authors： Pereira TN,Webb RI,Reilly PE,Seawright AA,Prakash AS

更新日期：1998-12-01 00:00:00

abstract：:The nucleotide sequence of a region located on the adenovirus type 5 genome between coordinates 59.9 and 62.5 has been determined. This region comprises the 3'-termini of mRNAs coding for the hexon and the DNA binding protein. The sequence reveals the presence of an open reading frame in r-strand transcripts downstrea...

journal_title：Nucleic acids research

authors： Kruijer W,van Schaik FM,Sussenbach JS

更新日期：1980-12-20 00:00:00

abstract：:Significant differences are observed between the CD spectra of eu- and heterochromatin prepared by fractionating mechanically sheared rat liver chromatin on linear sucrose density gradients. Heterochromatin has a broad positive CD at 270--280 nm, a cross-over point at 256 nmn, and a negative shoulder at 248 nm. Euchro...

journal_title：Nucleic acids research

authors： Moyer GH,Kay E,Austin GE

更新日期：1979-04-01 00:00:00

abstract：:Heterochromatin protein 1 (HP1) is an evolutionarily conserved chromosomal protein that binds to lysine 9-methylated histone H3 (H3K9me), a hallmark of heterochromatin. Although HP1 phosphorylation has been described in several organisms, the biological implications of this modification remain largely elusive. Here we...

journal_title：Nucleic acids research

authors： Nishibuchi G,Machida S,Osakabe A,Murakoshi H,Hiragami-Hamada K,Nakagawa R,Fischle W,Nishimura Y,Kurumizaka H,Tagami H,Nakayama J

更新日期：2014-11-10 00:00:00

abstract：:The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease activity and repair mechanisms in mammalian cells. However, analytical framework for extracting such information are lacking. Here, we present a novel computational platform called Rational InDel Meta-Analysis (RIMA) that en...

journal_title：Nucleic acids research

authors： Taheri-Ghahfarokhi A,Taylor BJM,Nitsch R,Lundin A,Cavallo AL,Madeyski-Bengtson K,Karlsson F,Clausen M,Hicks R,Mayr LM,Bohlooly-Y M,Maresca M

更新日期：2018-09-19 00:00:00

abstract：:Gold nanoparticle labels, combined with UV-visible optical absorption spectroscopic methods, are employed to probe the temperature-dependent solution properties of DNA triple helices. By using oligonucleotide-nanoparticle conjugates to characterize triplex denaturation, for the first time triplex to duplex melting tra...

journal_title：Nucleic acids research

authors： Murphy D,Eritja R,Redmond G

更新日期：2004-04-23 00:00:00

abstract：:A-tracts are functionally important DNA sequences which induce helix bending and have peculiar structural properties. While A-tract structure has been qualitatively well characterized, their mechanical properties remain controversial. A-tracts appear structurally rigid and resist nucleosome formation, but seem flexibl...

journal_title：Nucleic acids research

authors： Dršata T,Špačková N,Jurečka P,Zgarbová M,Šponer J,Lankaš F

更新日期：2014-06-01 00:00:00

abstract：:Large differences in plant genome sizes are mainly due to numerous events of insertions or deletions (indels). The balance between these events determines the evolutionary direction of genome changes. To address the question of what phenomena trigger these alterations, we compared the genomic sequences of two Arabidop...

journal_title：Nucleic acids research

authors： Ziolkowski PA,Koczyk G,Galganski L,Sadowski J

更新日期：2009-06-01 00:00:00

abstract：:MicroRNAs play important roles in many biological processes. Their aberrant expression can have oncogenic or tumor suppressor function directly participating to carcinogenesis, malignant transformation, invasiveness and metastasis. Indeed, miRNA profiles can distinguish not only between normal and cancerous tissue but...

journal_title：Nucleic acids research

authors： Cantini L,Bertoli G,Cava C,Dubois T,Zinovyev A,Caselle M,Castiglioni I,Barillot E,Martignetti L

更新日期：2019-03-18 00:00:00

abstract：:The available reagents for the attachment of functional moieties to plasmid DNA are limiting. Most reagents bind plasmid DNA in a non-sequence- specific manner, with undefined stoichiometry, and affect DNA charge and delivery properties or involve chemical modifications that abolish gene expression. The design and abi...

journal_title：Nucleic acids research

authors： Hertoghs KM,Ellis JH,Catchpole IR

更新日期：2003-10-15 00:00:00

abstract：:A novel method combining elements of suppression subtractive hybridization with high throughput differential screening permits the efficient and rapid cloning of rarely transcribed differentially expressed genes. The experimental strategy virtually excludes the possibility of isolating false positive clones. The poten...

journal_title：Nucleic acids research

authors： von Stein OD,Thies WG,Hofmann M

更新日期：1997-07-01 00:00:00

abstract：:The Diatom Expressed Sequence Tag (EST) Database was constructed to provide integral access to ESTs from these ecologically and evolutionarily interesting microalgae. It has now been updated with 130,000 Phaeodactylum tricornutum ESTs from 16 cDNA libraries and 77,000 Thalassiosira pseudonana ESTs from seven libraries...

journal_title：Nucleic acids research

authors： Maheswari U,Mock T,Armbrust EV,Bowler C

更新日期：2009-01-01 00:00:00

abstract：:The ribosomal proteins L4 and L22 form part of the peptide exit tunnel in the large ribosomal subunit. In Escherichia coli, alterations in either of these proteins can confer resistance to the macrolide antibiotic, erythromycin. The structures of the 30S as well as the 50S subunits from each antibiotic resistant mutan...

journal_title：Nucleic acids research

authors： O'Connor M,Gregory ST,Dahlberg AE

更新日期：2004-10-27 00:00:00

abstract：:This review presents detailed information about the structure of triplet repeat RNA and addresses the simple sequence repeats of normal and expanded lengths in the context of the physiological and pathogenic roles played in human cells. First, we discuss the occurrence and frequency of various trinucleotide repeats in...

journal_title：Nucleic acids research

authors： Krzyzosiak WJ,Sobczak K,Wojciechowska M,Fiszer A,Mykowska A,Kozlowski P

更新日期：2012-01-01 00:00:00

abstract：:The histone variant H2A.Z has been implicated in many biological processes, such as gene regulation and genome stability. Here, we present the identification of H2A.Z.2.2 (Z.2.2), a novel alternatively spliced variant of histone H2A.Z and provide a comprehensive characterization of its expression and chromatin incorpo...

journal_title：Nucleic acids research

authors： Bönisch C,Schneider K,Pünzeler S,Wiedemann SM,Bielmeier C,Bocola M,Eberl HC,Kuegel W,Neumann J,Kremmer E,Leonhardt H,Mann M,Michaelis J,Schermelleh L,Hake SB

更新日期：2012-07-01 00:00:00

abstract：:The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely rela...

journal_title：Nucleic acids research

authors： Zeng Y,Wang Y

更新日期：2006-01-01 00:00:00

abstract：:The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital importance for transcription, DNA replication and genome maintenance. Each of these activities is tightly regulated in both time and space. While we have a good understanding of chromatin organization in space, for example in fixe...

journal_title：Nucleic acids research

authors： Shaban HA,Seeber A

更新日期：2020-04-17 00:00:00

abstract：:Telomerase activity, not detectable in somatic cells but frequently activated during carcinogenesis, confers immortality to tumors. Mechanisms governing expression of the catalytic subunit hTERT, the limiting factor for telomerase activity, still remain unclear. We previously proposed a model in which the binding of t...

journal_title：Nucleic acids research

authors： Renaud S,Loukinov D,Alberti L,Vostrov A,Kwon YW,Bosman FT,Lobanenkov V,Benhattar J

更新日期：2011-02-01 00:00:00

abstract：:The interaction of Hoechst 33258 with the minor groove of the adenine-tract DNA duplex d(CTTTTGCAAAAG)2 has been studied in both D2O and H2O solutions by 1D and 2D 1H NMR spectroscopy. Thirty-one nuclear Overhauser effects between drug and nucleotide protons within the minor groove of the duplex, together with ring-cu...

journal_title：Nucleic acids research

authors： Searle MS,Embrey KJ

更新日期：1990-07-11 00:00:00

abstract：:The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenoty...

journal_title：Nucleic acids research

authors： Triman KL

更新日期：1996-01-01 00:00:00

abstract：:The role of thymine dimerization for previously reported U.V. induced conformational changes has been proved using acetophenone as sensitizer for the specific thymine dimerization upon irradiation at 313 nm. CD results demonstrate that formation of pyrimidine dimers cause typical conformational changes of the DNA B he...

journal_title：Nucleic acids research

authors： Lang H

更新日期：1975-02-01 00:00:00

abstract：:We present a www server for AUGUSTUS, a novel software program for ab initio gene prediction in eukaryotic genomic sequences. Our method is based on a generalized Hidden Markov Model with a new method for modeling the intron length distribution. This method allows approximation of the true intron length distribution m...

journal_title：Nucleic acids research

authors： Stanke M,Steinkamp R,Waack S,Morgenstern B

更新日期：2004-07-01 00:00:00

abstract：:The transcription of ribosomal genes in a cell line (Kc) derived from female D.melanogaster, has been investigated using hybridization probes prepared from restriction fragments of a cloned rDNA repeat with a 5 kb type I [Wellauer et al. (1978) Cell 14, 269-278] intron. Gels, of nuclear RNA that have been transferred ...

journal_title：Nucleic acids research

authors： Jolly DJ,Thomas CA Jr

更新日期：1980-01-11 00:00:00

abstract：:RegAnalyst is a user-friendly web interface that integrates MoPP (Motif Prediction Program), MyPatternFinder (pattern detection tool) and MycoRegDB (mycobacterial promoter and regulatory elements database). Since motif discovery is a challenging task, numerous tools have been developed over the past few years. Strikin...

journal_title：Nucleic acids research

authors： Sharma D,Mohanty D,Surolia A

更新日期：2009-07-01 00:00:00

abstract：:The crystal structure is reported of a complex between an ethyl derivative of the minor-groove drug furamidine and the dodecanucleotide duplex d(CGCGAATTCGCG)2, which has been refined to 1.85 A resolution and an R factor of 16.6% for data collected at -173 degreesC. An exceptionally large number (220) of water molecul...

journal_title：Nucleic acids research

authors： Guerri A,Simpson IJ,Neidle S

更新日期：1998-06-15 00:00:00

abstract：:Three computer programs are described which may be used to translate a DNA sequence into a protein sequence, back translate the protein sequence into an ambiguous DNA sequence, and then do pattern searching in the ambiguous sequence. The programs are written in the C programming language, have been compiled to run on ...

journal_title：Nucleic acids research

authors： Mount DW,Conrad B

更新日期：1984-01-11 00:00:00