Abstract：
:The transcription of ribosomal genes in a cell line (Kc) derived from female D.melanogaster, has been investigated using hybridization probes prepared from restriction fragments of a cloned rDNA repeat with a 5 kb type I [Wellauer et al. (1978) Cell 14, 269-278] intron. Gels, of nuclear RNA that have been transferred to diazotized paper and hybridized with labelled intron sequences, reveal both large (1-10 kb) transcripts and a discrete 325 base species. Berk-Sharp experiments [(1977) Cell 12, 721-732] reveal large transcripts that are homologous to intron sequences and extend into 28S sequences as well. However, while the abundance of 28S transcripts is about 50,000 copies per nucleus [Clark et al. (1977) Genetics 86, 789-800], these long transcripts are only present at 1-2 copies per nucleus and the 325 base species is only 10 times more abundant. In view of the fact that female cells have about 400 rDNA genes, 49% of which have type I introns, one must conclude either that transcription rarely occurs from the genes containing introns (the majority) or these transcripts are processed unusually rapidly. Transcripts homologous to the "non-transcribed spacer" region have been found, but their abundance is no higher.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Jolly DJ,Thomas CA Jrdoi
10.1093/nar/8.1.67subject
Has Abstractpub_date
1980-01-11 00:00:00pages
67-84issue
1eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract：:A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.6.1499
更新日期：1981-03-25 00:00:00
abstract：:We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly purified 5-MeC-DNA glycosylase complex were identified by Nanoelectrospray ionisation mass spectrometry to be identical to the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.16.3245
更新日期：1999-08-15 00:00:00
abstract：:To achieve drug resistance Leishmania parasite alters gene copy number by using its repeated sequences widely distributed through the genome. Even though homologous recombination (HR) is ascribed to maintain genome stability, this eukaryote exploits this potent mechanism driven by the Rad51 recombinase to form benefic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv118
更新日期：2015-03-11 00:00:00
abstract：:The effect of amino groups attached at positions 2 and 8 of the hypoxanthine moiety in the structure, reactivity and stability of DNA duplexes and triplexes is studied by means of quantum mechanical calculations, as well as extended molecular dynamics (MD) and thermodynamic integration (MD/TI) simulations. Theoretical...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.12.2522
更新日期：2001-06-15 00:00:00
abstract：:The Kluyveromyces lactis gene encoding the general transcription factor IIB (TFIIB) was isolated from a genomic library by complementation of the cold-sensitive phenotype conferred by a mutation in the SUA7 gene, which encodes TFIIB in Saccharomyces cerevisiae. DNA sequence analysis of the KI-SUA7 gene revealed a 357 ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.15.3413
更新日期：1993-07-25 00:00:00
abstract：:Alternative splicing expands the coding capacity of metazoan genes, and it was largely genetic studies in the fruit-fly Drosophila melanogaster that established the principle that regulated alternative splicing results in tissue- and stage-specific protein isoforms with different functions in development. Alternative ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr648
更新日期：2012-01-01 00:00:00
abstract：:Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt589
更新日期：2013-09-01 00:00:00
abstract：:The field bean, Vicia faba L. var. minor, possesses two sub-families of 11 S legumin genes named A and B. We isolated from a genomic library a B-type gene (LeB4) and determined its primary DNA sequence. Gene LeB4 codes for a 484 amino acid residue prepropolypeptide, encompassing a signal peptide of 22 amino acid resid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.6.2707
更新日期：1986-03-25 00:00:00
abstract：:Bacterial transcription factors often function as DNA-binding proteins that selectively activate or repress promoters, although the biochemical mechanisms vary. In most well-understood examples, activators function by either increasing the affinity of RNA polymerase (RNAP) for the target promoter, or by increasing the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1280
更新日期：2012-04-01 00:00:00
abstract：:CCG repeats are highly over-represented in exons of the human genome. Usually they are located in the 5' UTR but are also abundant in translated sequences. The CCG repeats are associated with three tri-nucleotide repeat disorders: Huntington's disease, myotonic dystrophy type 1 and chromosome X-linked mental retardati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks557
更新日期：2012-09-01 00:00:00
abstract：:In order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. A 16-mer oligonucleotide (ODN) was derivatized at the 5' prime through a 32P phosphate spacer with cholesterol, yielding a 32P-labeled amphiphatic cho...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.17.4695
更新日期：1991-09-11 00:00:00
abstract：:SLM2 and Sam68 are splicing regulator paralogs that usually overlap in function, yet only SLM2 and not Sam68 controls the Neurexin2 AS4 exon important for brain function. Herein we find that SLM2 and Sam68 similarly bind to Neurexin2 pre-mRNA, both within the mouse cortex and in vitro. Protein domain-swap experiments ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1277
更新日期：2017-04-20 00:00:00
abstract：:We have sequenced mouse tRNA genes from two recombinant lambda phage. An 1800 bp sequence from one phage contains 3 tRNA genes, potentially encoding tRNAAsp, tRNAGly, and tRNAGlu, separated by spacer sequences of 587 bp and 436 bp, respectively. The mouse tRNA gene cluster is homologous to a rat sequence (Sekiya et al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.24.8761
更新日期：1983-12-20 00:00:00
abstract：:Much effort has long been devoted to unraveling the coordinated cellular response to genotoxic insults. In view of the difficulty of obtaining human biological samples of homogeneous origin, I have established a set of stable human clones where one DNA repair gene has been stably silenced by means of RNA interference....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm195
更新日期：2007-01-01 00:00:00
abstract：:The allele frequency net database (http://www.allelefrequencies.net) is an online repository that contains information on the frequencies of immune genes and their corresponding alleles in different populations. The extensive variability observed in genes and alleles related to the immune system response and its signi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1128
更新日期：2011-01-01 00:00:00
abstract：:Sensitive detection of microsatellite instability (MSI) in tissue or liquid biopsies using next generation sequencing (NGS) has growing prognostic and predictive applications in cancer. However, the complexities of NGS make it cumbersome as compared to established multiplex-PCR detection of MSI. We present a new appro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1175
更新日期：2020-12-08 00:00:00
abstract：:Transgenic mice have proven to be an effective expression system for studying developmental control of the human fetal and adult beta-globin genes. In the current work we are interested in developing the transgenic mouse system for the study of the human embryonic beta-globin gene, epsilon. An epsilon-globin gene cons...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.18.5465
更新日期：1990-09-25 00:00:00
abstract：:The growing application of gene expression profiling demands powerful yet user-friendly bioinformatics tools to support systems-level data understanding. NetworkAnalyst was first released in 2014 to address the key need for interpreting gene expression data within the context of protein-protein interaction (PPI) netwo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz240
更新日期：2019-07-02 00:00:00
abstract：:Transcription factors are important gene regulators with distinctive roles in development, cell signaling and cell cycling, and they have been associated with many diseases. The ConTra v3 web server allows easy visualization and exploration of predicted transcription factor binding sites (TFBSs) in any genomic region ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx376
更新日期：2017-07-03 00:00:00
abstract：:Altering zinc bioavailability to bacterial pathogens is a key component of host innate immunity. Thus, the ability to sense and adapt to the alterations in zinc concentrations is critical for bacterial survival and pathogenesis. To understand the adaptive responses of group A Streptococcus (GAS) to zinc limitation and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1304
更新日期：2015-01-01 00:00:00
abstract：:The dissection of RNA editing mechanisms in PHYSARUM: mitochondria has been hindered by the absence of a soluble in vitro system. Based on our studies in isolated mitochondria, insertion of non-encoded nucleotides into PHYSARUM: mitochondrial RNAs is closely linked to transcription. Here we have fractionated mitochond...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.19.3695
更新日期：2000-10-01 00:00:00
abstract：:We describe a database of macromolecular motions meant to be of general use to the structural community. The database, which is accessible on the World Wide Web with an entry point at http://bioinfo.mbb.yale.edu/MolMovDB , attempts to systematize all instances of protein and nucleic acid movement for which there is at...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.18.4280
更新日期：1998-09-15 00:00:00
abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw580
更新日期：2016-09-30 00:00:00
abstract：:As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.12.3233
更新日期：1992-06-25 00:00:00
abstract：:Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku303
更新日期：2014-06-01 00:00:00
abstract：:We have developed an effective, easy-to-use two-step system for the site-directed insertion of large genetic constructs into arbitrary positions in the Escherichia coli chromosome. The system uses lambda-Red mediated recombineering accompanied by the introduction of double-strand DNA breaks in the chromosome and a don...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1193
更新日期：2010-04-01 00:00:00
abstract：:In order to examine the nature of the complex formation between the ribosomal protein S1 and nucleic acids three methods were used: Inhibition of the reaction of n-ethyl[2.3 14C]-maleimide with S1 by the addition of oligonucleotides; adsorption of the complexes to nitrocellulose filters; and equilibrium dialysis. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.10.3627
更新日期：1977-10-01 00:00:00
abstract：:Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq456
更新日期：2010-10-01 00:00:00
abstract：:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.11.1549
更新日期：1974-11-01 00:00:00
abstract：:FRS1 and FRS2, the structural genes encoding the large (alpha) and small (beta) subunits of yeast phenylalanyl-tRNA synthetase (PheRS) were placed under the control of the lacZ promoter by creating an artificial operon. The FRS2 gene was fused next to the promoter, followed by a 14 base pair intergenic sequence contai...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.8.2087
更新日期：1990-04-25 00:00:00
