Abstract:
:We have sequenced mouse tRNA genes from two recombinant lambda phage. An 1800 bp sequence from one phage contains 3 tRNA genes, potentially encoding tRNAAsp, tRNAGly, and tRNAGlu, separated by spacer sequences of 587 bp and 436 bp, respectively. The mouse tRNA gene cluster is homologous to a rat sequence (Sekiya et al., 1981, Nucleic Acids Res. 9, 2239-2250). The mouse and rat tRNAAsp and tRNAGly coding regions are identical. The tRNAGlu coding regions differ at two positions. The flanking sequences contain 3 non-homologous areas: a c. 100 bp insertion in the first mouse spacer, short tandemly repeated sequences in the second spacers and unrelated sequences at the 3' ends of the clusters. In contrast, most of the flanking regions are homologous, consisting of strings of consecutive, identical residues (5-17 bp) separated by single base differences and short insertions/deletions. The latter are often associated with short repeats. The homology of the flanking regions is c. 75%, similar to other murine genes. The second lambda clone contains a solitary mouse tRNAAsp gene. The coding region is identical to that of the clustered tRNAAsp gene. The 5' flanking regions of the two genes contain homologous areas (10-25 bp) separated by unrelated sequences. Overall, the flanking regions of the two mouse tRNAAsp genes are less homologous than those of the mouse and rat clusters.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Looney JE,Harding JDdoi
10.1093/nar/11.24.8761subject
Has Abstractpub_date
1983-12-20 00:00:00pages
8761-75issue
24eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::Mitochondrial DNA (mtDNA) resides in a high ROS environment and suffers more mutations than its nuclear counterpart. Increasing evidence suggests that mtDNA mutations are not the results of direct oxidative damage, rather are caused, at least in part, by DNA replication errors. To understand how the mtDNA replicase, P...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1018
更新日期:2020-01-24 00:00:00
abstract::Abasic sites (AP-sites) are frequent DNA lesions, arising by spontaneous base hydrolysis or as intermediates of base excision repair (BER). The hemiacetal at the anomeric centre renders them chemically reactive, which presents a challenge to biochemical and structural investigation. Chemically more stable AP-site anal...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1520
更新日期:2016-03-18 00:00:00
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journal_title:Nucleic acids research
pub_type:
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh022
更新日期:2004-02-18 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/4.4.955
更新日期:1977-04-01 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gks267
更新日期:2012-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky632
更新日期:2018-10-12 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.10.3439
更新日期:1985-05-24 00:00:00
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journal_title:Nucleic acids research
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更新日期:2013-12-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1975-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkaa135
更新日期:2020-04-17 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1075
更新日期:2007-01-01 00:00:00
abstract::Although subtelomeric regions in humans are heterochromatic, the epigenetic nature of human telomeres remains controversial. This controversy might have been influenced by the confounding effect of subtelomeric regions and interstitial telomeric sequences (ITSs) on telomeric chromatin structure analyses. In addition, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky006
更新日期:2018-03-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.20.4050
更新日期:1999-10-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.6.2523
更新日期:1986-03-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr513
更新日期:2011-10-01 00:00:00
abstract::We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated DNA. To study the effects of the phosphorylation/dephosphorylation of MDBP-2-H1 on the expression of the avian vitellogeni...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.10.1816
更新日期:1996-05-15 00:00:00
abstract::Sodium is one of the most abundant metals in the environment and in biology, playing critical ecological and physiological roles. Na+ is also the most common buffer salt for nucleic acids research, while its specific interaction with DNA has yet to be fully studied. Herein, we probe a highly selective and robust Na+ a...
journal_title:Nucleic acids research
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更新日期:2016-12-01 00:00:00
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更新日期:2008-10-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-06-16 00:00:00
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更新日期:1996-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2015-07-27 00:00:00
abstract::Sequences from -3500 to +1365 of the chicken histone H5 gene have been analyzed for the presence of cis-acting elements in H5 expressing (transformed CFU-E) and non-expressing cells (fibroblasts). The region from -3500 to -115 had little effect on transcription. Proximal upstream sequences contain a negative element (...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1989-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1999-01-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/7.6.1541
更新日期:1979-11-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-01-10 00:00:00