Abstract:
:The Kluyveromyces lactis gene encoding the general transcription factor IIB (TFIIB) was isolated from a genomic library by complementation of the cold-sensitive phenotype conferred by a mutation in the SUA7 gene, which encodes TFIIB in Saccharomyces cerevisiae. DNA sequence analysis of the KI-SUA7 gene revealed a 357 amino acid open reading frame that is 67% identical (81% overall similarity) to S. cerevisiae TFIIB. Comparison with other eukaryotic TFIIBs indicated that the most highly conserved sequence is located adjacent to the Zn-finger motif near the N-terminus. A plasmid shuffle system was used to replace the essential Sc-SUA7 gene with KI-SUA7 in S.cerevisiae. The resulting strain was viable and phenotypically indistinguishable from the normal strain. However, transcription start site selection at the ADH1 locus, shown previously to be affected by mutations in Sc-SUA7, was affected by K.lactis TFIIB. This result provides further evidence that TFIIB is a principal determinant of start site selection in yeast.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Na JG,Hampsey Mdoi
10.1093/nar/21.15.3413subject
Has Abstractpub_date
1993-07-25 00:00:00pages
3413-7issue
15eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Mammalian cryptochromes (Crys) are essential circadian clock factors implicated in diverse clock-independent physiological functions, including DNA damage responses. Here we show that Cry1 modulates the ATR-mediated DNA damage checkpoint (DDC) response by interacting with Timeless (Tim) in a time-of-day-dependent mann...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku094
更新日期:2014-04-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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doi:10.1093/nar/13.24.8893
更新日期:1985-12-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg295
更新日期:2003-04-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1992-02-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-02-07 00:00:00
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journal_title:Nucleic acids research
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更新日期:2002-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1985-05-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq660
更新日期:2010-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm641
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.15.3911
更新日期:1992-08-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-10-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1057
更新日期:2011-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type:
doi:10.1093/nar/17.suppl.r283
更新日期:1989-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx425
更新日期:2017-07-03 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-08-09 00:00:00
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journal_title:Nucleic acids research
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更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2016-02-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1996-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1975-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.11.4037
更新日期:1989-06-12 00:00:00
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journal_title:Nucleic acids research
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更新日期:1985-09-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2012-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-06-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-11-02 00:00:00