Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.


:Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from the actinomycin intercalation site in a molecule of sequence d[ATATATAGCTATATAT] that does not occur in d[AAAAAAAGCTTTTTTT]. The experiments demonstrate that DNase I rate enhancements associated with actinomycin D binding are caused by ligand alteration of equilibrium DNA structure.


Nucleic Acids Res


Nucleic acids research


Huang YQ,Rehfuss RP,LaPlante SR,Boudreau E,Borer PN,Lane MJ




Has Abstract


1988-12-09 00:00:00












  • Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism.

    abstract::The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We report here that these transcripts produce three forms of MTH1 polypeptides (...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Oda H,Taketomi A,Maruyama R,Itoh R,Nishioka K,Yakushiji H,Suzuki T,Sekiguchi M,Nakabeppu Y

    更新日期:1999-11-15 00:00:00

  • Autoregulation of phage mu transposase at the level of translation.

    abstract::The bacteriophage Mu A and B genes, which lie adjacent to each other and are colinear on the phage genome, encode proteins that catalyze efficient transposition of Mu DNA. We show that the molar ratio of A and B proteins is approximately 1:20 in extracts prepared after induction of cells containing a Mu lysogen or a p...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Parsons RL,Harshey R

    更新日期:1988-12-09 00:00:00

  • BiSearch: primer-design and search tool for PCR on bisulfite-treated genomes.

    abstract::Bisulfite genomic sequencing is the most widely used technique to analyze the 5-methylation of cytosines, the prevalent covalent DNA modification in mammals. The process is based on the selective transformation of unmethylated cytosines to uridines. Then, the investigated genomic regions are PCR amplified, subcloned a...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tusnády GE,Simon I,Váradi A,Arányi T

    更新日期:2005-01-13 00:00:00

  • ATPase activity of the UvrA and UvrAB protein complexes of the Escherichia coli UvrABC endonuclease.

    abstract::We have analyzed the ATPase activity exhibited by the UvrABC DNA repair complex. The UvrA protein is an ATPase whose lack of DNA dependence may be related to the ATP induced monomer-dimer transitions. ATP induced dimerization may be responsible for the enhanced DNA binding activity observed in the presence of ATP. Alt...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Oh EY,Claassen L,Thiagalingam S,Mazur S,Grossman L

    更新日期:1989-06-12 00:00:00

  • Single-cell chromosomal imbalances detection by array CGH.

    abstract::Genomic imbalances are a major cause of constitutional and acquired disorders. Therefore, aneuploidy screening has become the cornerstone of preimplantation, prenatal and postnatal genetic diagnosis, as well as a routine aspect of the diagnostic workup of many acquired disorders. Recently, array comparative genomic hy...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Le Caignec C,Spits C,Sermon K,De Rycke M,Thienpont B,Debrock S,Staessen C,Moreau Y,Fryns JP,Van Steirteghem A,Liebaers I,Vermeesch JR

    更新日期:2006-05-12 00:00:00

  • The Pch2 AAA+ ATPase promotes phosphorylation of the Hop1 meiotic checkpoint adaptor in response to synaptonemal complex defects.

    abstract::Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Herruzo E,Ontoso D,González-Arranz S,Cavero S,Lechuga A,San-Segundo PA

    更新日期:2016-09-19 00:00:00

  • Enhancement of ribosomal frameshifting by oligonucleotides targeted to the HIV gag-pol region.

    abstract::The pol gene of all retroviruses is expressed as a gag-pol fusion protein which is proteolytically processed to produce all viral enzymes. In the human immunodeficiency virus (HIV), the gag and pol genes overlap by 241 nucleotides with pol in the -1 phase with respect to gag. The gag-pol fusion is produced via a -1 ri...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Vickers TA,Ecker DJ

    更新日期:1992-08-11 00:00:00

  • Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.

    abstract::Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has be...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Upcroft P,Carter B,Kidson C

    更新日期:1980-06-25 00:00:00

  • Deoxyribozymes that recode sequence information.

    abstract::Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered liga...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tabor JJ,Levy M,Ellington AD

    更新日期:2006-04-28 00:00:00

  • The Dictyostelium discoideum RNA-dependent RNA polymerase RrpC silences the centromeric retrotransposon DIRS-1 post-transcriptionally and is required for the spreading of RNA silencing signals.

    abstract::Dictyostelium intermediate repeat sequence 1 (DIRS-1) is the founding member of a poorly characterized class of retrotransposable elements that contain inverse long terminal repeats and tyrosine recombinase instead of DDE-type integrase enzymes. In Dictyostelium discoideum, DIRS-1 forms clusters that adopt the functio...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wiegand S,Meier D,Seehafer C,Malicki M,Hofmann P,Schmith A,Winckler T,Földesi B,Boesler B,Nellen W,Reimegård J,Käller M,Hällman J,Emanuelsson O,Avesson L,Söderbom F,Hammann C

    更新日期:2014-03-01 00:00:00

  • Method for multiplex cellular detection of mRNAs using quantum dot fluorescent in situ hybridization.

    abstract::The photostability and narrow emission spectra of non-organic quantum dot fluorophores (QDs) make them desirable candidates for fluorescent in situ hybridization (FISH) to study the expression of specific mRNA transcripts. We developed a novel method for direct QD labeling of modified oligonucleotide probes through st...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chan P,Yuen T,Ruf F,Gonzalez-Maeso J,Sealfon SC

    更新日期:2005-10-13 00:00:00

  • Quantitative analysis of mRNA amplification by in vitro transcription.

    abstract::Effective transcript profiling in animal systems requires isolation of homogenous tissue or cells followed by faithful mRNA amplification. Linear amplification based on cDNA synthesis and in vitro transcription is reported to maintain representation of mRNA levels, however, quantitative data demonstrating this as well...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Baugh LR,Hill AA,Brown EL,Hunter CP

    更新日期:2001-03-01 00:00:00

  • Dissecting the functional program of Escherichia coli promoters: the combined mode of action of Lac repressor and AraC activator.

    abstract::The mode of action of regulated promoters is largely determined by kinetic parameters which govern the interaction between promoters and proteins involved in induction and repression of transcription. To gain insight into the interplay between positively and negatively acting transcriptional regulators, in this case A...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lutz R,Lozinski T,Ellinger T,Bujard H

    更新日期:2001-09-15 00:00:00

  • Bacteriophage T4 RNA ligase: preparation of a physically homogeneous, nuclease-free enzyme from hyperproducing infected cells.

    abstract::Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells infected by the double mutant, RNA ligase was purified to homogeneity with a 20% yield. Unlike previous preparations of t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Higgins NP,Geballe AP,Snopek TJ,Sugino A,Cozzarelli NR

    更新日期:1977-09-01 00:00:00

  • Genome maintenance in Saccharomyces cerevisiae: the role of SUMO and SUMO-targeted ubiquitin ligases.

    abstract::The genome of the cell is often exposed to DNA damaging agents and therefore requires an intricate well-regulated DNA damage response (DDR) to overcome its deleterious effects. The DDR needs proper regulation for its timely activation, repression, as well as appropriate choice of repair pathway. Studies in Saccharomyc...

    journal_title:Nucleic acids research

    pub_type: 杂志文章,评审


    authors: Jalal D,Chalissery J,Hassan AH

    更新日期:2017-03-17 00:00:00

  • Mouse DNA 'fingerprints': analysis of chromosome localization and germ-line stability of hypervariable loci in recombinant inbred strains.

    abstract::Human minisatellite probes cross-hybridize to mouse DNA and detect multiple variable loci. The resulting DNA "fingerprints" vary substantially between inbred strains but relatively little within an inbred strain. By studying the segregation of variable DNA fragments in BXD recombinant inbred strains of mice, at least ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jeffreys AJ,Wilson V,Kelly R,Taylor BA,Bulfield G

    更新日期:1987-04-10 00:00:00

  • A test of 'polymerase handover' as a mechanism for stimulating initiation by RNA polymerase I.

    abstract::On the tandemly linked ribosomal genes of Xenopus laevis, the RNA polymerase transcribes past the 3' end of the 40S coding region and terminates at T3 just upstream of the gene promoter. The close proximity of T3 to the gene promoter, and the functional interdependence of these two elements, has led to the suggestion ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lucchini R,Reeder RH

    更新日期:1989-01-11 00:00:00

  • Conditional degradation of SDE2 by the Arg/N-End rule pathway regulates stress response at replication forks.

    abstract::Multiple pathways counteract DNA replication stress to prevent genomic instability and tumorigenesis. The recently identified human SDE2 is a genome surveillance protein regulated by PCNA, a DNA clamp and processivity factor at replication forks. Here, we show that SDE2 cleavage after its ubiquitin-like domain generat...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Rageul J,Park JJ,Jo U,Weinheimer AS,Vu TTM,Kim H

    更新日期:2019-05-07 00:00:00

  • Use of an internal ribosome entry site for bicistronic expression of Cre recombinase or rtTA transactivator.

    abstract::Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lindeberg J,Ebendal T

    更新日期:1999-03-15 00:00:00

  • Hypermethylated-capped selenoprotein mRNAs in mammals.

    abstract::Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5'-end m(7)G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear a hypermethylated c...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wurth L,Gribling-Burrer AS,Verheggen C,Leichter M,Takeuchi A,Baudrey S,Martin F,Krol A,Bertrand E,Allmang C

    更新日期:2014-07-01 00:00:00

  • Topoisomerase II minimizes DNA entanglements by proofreading DNA topology after DNA strand passage.

    abstract::By transporting one DNA double helix (T-segment) through a double-strand break in another (G-segment), topoisomerase II reduces fractions of DNA catenanes, knots and supercoils to below equilibrium values. How DNA segments are selected to simplify the equilibrium DNA topology is enigmatic, and the biological relevance...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Martínez-García B,Fernández X,Díaz-Ingelmo O,Rodríguez-Campos A,Manichanh C,Roca J

    更新日期:2014-02-01 00:00:00

  • Sinefungin resistance of Saccharomyces cerevisiae arising from Sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase.

    abstract::The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zheng S,Shuman S,Schwer B

    更新日期:2007-01-01 00:00:00

  • Conservation, localization and expression of HopZ, a protein involved in adhesion of Helicobacter pylori.

    abstract::From a sarkosyl-insoluble outer membrane fraction prepared from the Helicobacter pylori strain ATCC 43504, 19 proteins could be sequenced N-terminally by Edman degradation. Oligonucleotides were deduced and used for screening of a genomic library. From the isolated genes, five code for different members of a H.pylori ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Peck B,Ortkamp M,Diehl KD,Hundt E,Knapp B

    更新日期:1999-08-15 00:00:00

  • The EROP-Moscow oligopeptide database.

    abstract::Natural oligopeptides may regulate nearly all vital processes. To date, the chemical structures of nearly 6000 oligopeptides have been identified from >1000 organisms representing all the biological kingdoms. We have compiled the known physical, chemical and biological properties of these oligopeptides--whether synthe...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zamyatnin AA,Borchikov AS,Vladimirov MG,Voronina OL

    更新日期:2006-01-01 00:00:00

  • A novel mechanism of selectivity against AZT by the human mitochondrial DNA polymerase.

    abstract::Native nucleotides show a hyperbolic concentration dependence of the pre-steady-state rate of incorporation while maintaining concentration-independent amplitude due to fast, largely irreversible pyrophosphate release. The kinetics of 3'-azido-2',3'-dideoxythymidine (AZT) incorporation exhibit an increase in amplitude...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hanes JW,Johnson KA

    更新日期:2007-01-01 00:00:00

  • The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli.

    abstract::The CRISPR/Cas adaptive immune system provides resistance against phages and plasmids in Archaea and Bacteria. CRISPR loci integrate short DNA sequences from invading genetic elements that provide small RNA-mediated interference in subsequent exposure to matching nucleic acids. In Streptococcus thermophilus, it was pr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sapranauskas R,Gasiunas G,Fremaux C,Barrangou R,Horvath P,Siksnys V

    更新日期:2011-11-01 00:00:00

  • Structural activation of the transcriptional repressor EthR from Mycobacterium tuberculosis by single amino acid change mimicking natural and synthetic ligands.

    abstract::Ethionamide is an antituberculous drug for the treatment of multidrug-resistant Mycobacterium tuberculosis. This antibiotic requires activation by the monooxygenase EthA to exert its activity. Production of EthA is controlled by the transcriptional repressor EthR, a member of the TetR family. The sensitivity of M. tub...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Carette X,Blondiaux N,Willery E,Hoos S,Lecat-Guillet N,Lens Z,Wohlkönig A,Wintjens R,Soror SH,Frénois F,Dirié B,Villeret V,England P,Lippens G,Deprez B,Locht C,Willand N,Baulard AR

    更新日期:2012-04-01 00:00:00

  • 3' processing of pre-mRNA plays a major role in proliferation-dependent regulation of histone gene expression.

    abstract::A short histone-like fusion RNA, generated when the RNA 3' processing signal from a mouse histone H4 gene is inserted into a heterologous transcription unit, becomes correctly down-regulated in G1-arrested cells of a temperature-sensitive mouse mastocytoma cell cycle mutant (21-Tb; Stauber et al., EMBO J. 5, 3297-3303...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Stauber C,Schümperli D

    更新日期:1988-10-25 00:00:00

  • rDNA in Locusta migratoria is very variable: two introns and extensive restriction site polymorphisms in the spacer.

    abstract::Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schäfer M,Kunz W

    更新日期:1985-02-25 00:00:00

  • EpoDB: a prototype database for the analysis of genes expressed during vertebrate erythropoiesis.

    abstract::EpoDB is a database of genes expressed in vertebrate red blood cells. It is also a prototype for the creation of cell and tissue-specific databases from multiple external sources. The information in EpoDB obtained from GenBank, SWISS-PROT, Transfac, TRRD and GERD is curated to provide high quality data for sequence an...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Stoeckert CJ Jr,Salas F,Brunk B,Overton GC

    更新日期:1999-01-01 00:00:00