Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.

Abstract:

:Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from the actinomycin intercalation site in a molecule of sequence d[ATATATAGCTATATAT] that does not occur in d[AAAAAAAGCTTTTTTT]. The experiments demonstrate that DNase I rate enhancements associated with actinomycin D binding are caused by ligand alteration of equilibrium DNA structure.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Huang YQ,Rehfuss RP,LaPlante SR,Boudreau E,Borer PN,Lane MJ

doi

10.1093/nar/16.23.11125

subject

Has Abstract

pub_date

1988-12-09 00:00:00

pages

11125-39

issue

23

eissn

0305-1048

issn

1362-4962

journal_volume

16

pub_type

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