Abstract:
:Membraneless organelles (MOs) are dynamic liquid condensates that host a variety of specific cellular processes, such as ribosome biogenesis or RNA degradation. MOs form through liquid-liquid phase separation (LLPS), a process that relies on multivalent weak interactions of the constituent proteins and other macromolecules. Since the first discoveries of certain proteins being able to drive LLPS, it emerged as a general mechanism for the effective organization of cellular space that is exploited in all kingdoms of life. While numerous experimental studies report novel cases, the computational identification of LLPS drivers is lagging behind, and many open questions remain about the sequence determinants, composition, regulation and biological relevance of the resulting condensates. Our limited ability to overcome these issues is largely due to the lack of a dedicated LLPS database. Therefore, here we introduce PhaSePro (https://phasepro.elte.hu), an openly accessible, comprehensive, manually curated database of experimentally validated LLPS driver proteins/protein regions. It not only provides a wealth of information on such systems, but improves the standardization of data by introducing novel LLPS-specific controlled vocabularies. PhaSePro can be accessed through an appealing, user-friendly interface and thus has definite potential to become the central resource in this dynamically developing field.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Mészáros B,Erdős G,Szabó B,Schád É,Tantos Á,Abukhairan R,Horváth T,Murvai N,Kovács OP,Kovács M,Tosatto SCE,Tompa P,Dosztányi Z,Pancsa Rdoi
10.1093/nar/gkz848subject
Has Abstractpub_date
2020-01-08 00:00:00pages
D360-D367issue
D1eissn
0305-1048issn
1362-4962pii
5587636journal_volume
48pub_type
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