Abstract:
:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.Images
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Jaye M,de la Salle H,Schamber F,Balland A,Kohli V,Findeli A,Tolstoshev P,Lecocq JPdoi
10.1093/nar/11.8.2325subject
Has Abstractpub_date
1983-04-25 00:00:00pages
2325-35issue
8eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::The structure of eukaryotic chromatin has been investigated by isolating and analyzing the "accessible" DNA fraction of rat liver chromatin. This DNA fraction has been isolated by titrating the chromatin with the protese-resistant D isomer of polylysine to bind the "accessible" DNA sites. After removal of chromosomal ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.5.667
更新日期:1975-05-01 00:00:00
abstract::Glucocorticoid (GC) signaling synchronizes the circadian rhythm of individual peripheral cells and induces the expression of circadian genes, including Period1 (Per1) and Period2 (Per2). However, no GC response element (GRE) has been reported in the Per2 promoter region. Here we report the molecular mechanisms of Per2...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt307
更新日期:2013-07-01 00:00:00
abstract::Mutations in the gene of human RNase T2 are associated with white matter disease of the human brain. Although brain abnormalities (bilateral temporal lobe cysts and multifocal white matter lesions) and clinical symptoms (psychomotor impairments, spasticity and epilepsy) are well characterized, the pathomechanism of RN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks614
更新日期:2012-09-01 00:00:00
abstract::The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) is Europe's primary nucleotide-sequence repository. The ENA consists of three main databases: the Sequence Read Archive (SRA), the Trace Archive and EMBL-Bank. The objective of ENA is to support and promote the use of nucleotide sequencing as an experimen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq967
更新日期:2011-01-01 00:00:00
abstract::An adequate supply of nucleotides is essential for DNA replication and DNA repair. Moreover, inhibition of TTP synthesis can cause cell death by a poorly characterized mechanism called thymine-less death. In the yeast Saccharomyces cerevisiae, the genes encoding thymidylate synthetase (CDC21) and thymidylate kinase (C...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki219
更新日期:2005-01-28 00:00:00
abstract::Genome-wide transcriptome profiling has enabled non-supervised classification of tumours, revealing different sub-groups characterized by specific gene expression features. However, the biological significance of these subtypes remains for the most part unclear. We describe herein an interactive platform, Minimum Span...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx338
更新日期:2017-07-27 00:00:00
abstract::To investigate the mechanism of N4-aminocytidine-induced mutagenesis, N'-alkyl-N4-aminocytidines and N4-alkyl-N4-aminocytidines were prepared and their mutagenicity on bacteria were assayed. N'-Methyl-N4-aminocytidine, N'-(2-hydroxyethyl)-N4-aminocytidine and N',N'-dimethyl-N4-aminocytidine showed direct-acting mutage...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.24.8893
更新日期:1985-12-20 00:00:00
abstract::Heat shock genes are activated by the binding of the heat shock transcription factor (HSF) to heat shock elements (HSEs), consisting of arrays of the 5-bp unit NGAAN arranged as inverted repeats. Here, we have investigated the interaction of the 5-bp unit with HSFs of Drosophila and Saccharomyces. Mutations within the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.2.167
更新日期:1994-01-25 00:00:00
abstract::SPRING (http://algorithm.cs.nthu.edu.tw/tools/SPRING/) is a tool for the analysis of genome rearrangement between two chromosomal genomes using reversals and/or block-interchanges. SPRING takes two or more chromosomes as its input and then computes a minimum series of reversals and/or block-interchanges between any tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl169
更新日期:2006-07-01 00:00:00
abstract::ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-locus analyses of inb...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw505
更新日期:2016-09-30 00:00:00
abstract::The Drosophila mophogenetic protein Bicoid (Bcd) can activate transcription in a concentration-dependent manner in embryos. It contains a self-inhibitory domain that can interact with the co-repressor Sin3A. In this report, we study a Bcd mutant, Bcd(A57-61), which has a strengthened self-inhibitory function and is un...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki691
更新日期:2005-07-19 00:00:00
abstract::We have determined the nucleotide sequence at the 3' end of the vesicular stomatitis virus (VSV) polymerase or L gene and compared it to that obtained from a defective interfering particle (DI) RNA generated by this virus. The latter (DI 0.50) contains a large internal deletion within this gene. The deletion begins ex...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.21.6919
更新日期:1982-11-11 00:00:00
abstract::Characterization of the epigenetic status of individual cells remains a challenge. Current sequencing approaches have limited coverage, and it is difficult to assign an epigenetic status to the transcription state of individual gene alleles in the same cell. To address these limitations, a targeted microscopy-based ep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkab009
更新日期:2021-01-28 00:00:00
abstract::Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are lar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.15.3851
更新日期:1981-08-11 00:00:00
abstract::RNA sequencing has become an important method to perform hypothesis-free characterization of global gene expression. One of the limitations of RNA sequencing is that most sequence reads represent highly expressed transcripts, whereas low level transcripts are challenging to detect. To combine the benefits of tradition...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks816
更新日期:2013-01-07 00:00:00
abstract::A promoter with the potential to adopt a 50 basepair (bp) cruciform spanning from -19 to -69 has been constructed in the plasmid pBR322 tetracycline resistance gene (tet) by forming an inverted repeat from '-35' sequences. Compared to a control promoter, the sequence of this cruciform promoter differs only by a 22 bp ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.14.5537
更新日期:1989-07-25 00:00:00
abstract::In many eukaryotic mRNAs one or more short 'upstream' open reading frames, uORFs, precede the initiator of the main coding sequence. Upstream ORFs are functionally diverse as illustrated by their variety of features in polyamine pathway biosynthetic mRNAs. Their propensity to act as sensors for regulatory circuits and...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkp1037
更新日期:2010-01-01 00:00:00
abstract::RNA-protein complexes are essential in mediating important fundamental cellular processes, such as transport and localization. In particular, ncRNA-protein interactions play an important role in post-transcriptional gene regulation like mRNA localization, mRNA stabilization, poly-adenylation, splicing and translation....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv020
更新日期:2015-02-18 00:00:00
abstract::We demonstrate compaction of DNA with nanoscale biomimetic constructs which are robust synthetic analogs of globular proteins. These constructs are approximately 15 nm in diameter, shell crosslinked knedel-like (SCKs) nanoparticles, which are prepared by covalent stabilization of amphiphilic di-block co-polymer micell...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.14.2966
更新日期:1999-07-15 00:00:00
abstract::Granulocyte macrophage-colony stimulating factor (GM-CSF) is produced by T cells, but not B cells, in response to immune signals. GM-CSF gene activation in response to T-cell stimulation requires remodelling of chromatin associated with the gene promoter, and these changes do not occur in B cells. While the CpG methyl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn117
更新日期:2008-05-01 00:00:00
abstract::Telomeric DNAs consist of tandem repeats of G-clusters such as TTAGGG and TG1-3, which are the human and yeast repeat sequences, respectively. In the yeast Saccharomyces cerevisiae, the telomeric repeats are non-nucleosomal, whereas in humans, they are organized in tightly packaged nucleosomes. However, previous in vi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1006
更新日期:2014-02-01 00:00:00
abstract::Large-scale sequencing studies discovered substantial genetic variants occurring in enhancers which regulate genes via long range chromatin interactions. Importantly, such variants could affect enhancer regulation by changing transcription factor bindings or enhancer hijacking, and in turn, make an essential contribut...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx920
更新日期:2018-01-04 00:00:00
abstract::The eukaryotic linear motif (ELM) resource is a repository of manually curated experimentally validated short linear motifs (SLiMs). Since the initial release almost 20 years ago, ELM has become an indispensable resource for the molecular biology community for investigating functional regions in many proteins. In this...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1030
更新日期:2020-01-08 00:00:00
abstract::Triple helix-forming oligonucleotides covalently linked to topoisomerase I inhibitors, in particular the antitumor agent camptothecin, trigger topoisomerase I-mediated DNA cleavage selectively in the proximity of the binding site of the oligonucleotide vector. In the present study, we have performed a systematic analy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg457
更新日期:2003-07-15 00:00:00
abstract::A comprehensive listing is made of posttranscriptionally modified nucleosides from RNA reported in the literature through mid-1994. Included are chemical structures, common names, symbols, Chemical Abstracts registry numbers (for ribonucleoside and corresponding base), Chemical Abstracts Index Name, phylogenetic sourc...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/22.12.2183
更新日期:1994-06-25 00:00:00
abstract::Three peaks of methyltransferase activity specific for MNNG alkylated DNA have been identified from extracts of chemically adapted M. luteus. They are designated as TI to TIII in order to their elution from a Sephadex G-75 column. The first one of these peaks has been purified to homogeneity. TI, is an inducible, unus...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.22.9471
更新日期:1987-11-25 00:00:00
abstract::Generally, the presence of a consensus sequence in the promoter of a gene is taken as indication for regulation by the transcription factor that binds to this sequence. In light of the recent developments in genome research, we were interested to what extent this supposition is valid. We examined the relationship betw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf698
更新日期:2002-12-15 00:00:00
abstract::A very mild and efficient procedure has been developed for the preparation of C-5 substituted deoxyuridines. The substituent carries a masked primary aliphatic amino group. These compounds are readily converted into their phosphoramidites and can be used to prepare oligonucleotides carrying one or more aliphatic amino...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.12.4857
更新日期:1987-06-25 00:00:00
abstract::The role of the cyclic-AMP (cAMP) responsive element (CRE) in eukaryotic gene transcription was investigated in several cell lines transfected by constructs containing the chloramphenicol acetyltransferase (CAT) gene linked to the three different promoters, simian virus (SV) 40, human c-Ha-ras-1, or chicken beta-actin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.4.1521
更新日期:1989-02-25 00:00:00
abstract::The 5'-cap of spliceosomal small nuclear RNAs, some small nucleolar RNAs and of telomerase RNA was found to be hypermethylated in vivo. The Trimethylguanosine Synthase 1 (TGS1) mediates this conversion of the 7-methylguanosine-cap to the 2,2,7-trimethylguanosine (m(3)G)-cap during maturation of the RNPs. For mammalian...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp249
更新日期:2009-07-01 00:00:00