Sequence signal involved in the generation of an internally deleted defective interfering RNA from vesicular stomatitis virus.

abstract：:Biological pathways can provide key information on the organization of biological systems. ArrayXPath (http://www.snubi.org/software/ArrayXPath/) is a web-based service for mapping and visualizing microarray gene-expression data for integrated biological pathway resources using Scalable Vector Graphics (SVG). By integ...

journal_title：Nucleic acids research

authors： Chung HJ,Kim M,Park CH,Kim J,Kim JH

更新日期：2004-07-01 00:00:00

abstract：:Eukaryotic cells possess several distinct mismatch repair pathways. A mismatch can be introduced in retroviral double-stranded DNA by a pre-existing mutation within the primer binding site (PBS) of the viral RNA genome. In order to evaluate mismatch repair of retroviral double-stranded DNA, Moloney leukemia virus (MLV...

journal_title：Nucleic acids research

authors： Tang LY,Zhang J

更新日期：2000-06-15 00:00:00

abstract：:We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutationa...

journal_title：Nucleic acids research

authors： Tsukahara T,Casciato C,Helfman DM

更新日期：1994-06-25 00:00:00

abstract：:The RNA-Seq technology has revolutionized transcriptome characterization not only by accurately quantifying gene expression, but also by the identification of novel transcripts like chimeric fusion transcripts. The 'fusion' or 'chimeric' transcripts have improved the diagnosis and prognosis of several tumors, and have...

journal_title：Nucleic acids research

authors： Li Y,Heavican TB,Vellichirammal NN,Iqbal J,Guda C

更新日期：2017-07-27 00:00:00

abstract：:DNA polymerase mu is a member of the mammalian pol X family and reduces deletion during chromosome break repair by nonhomologous end joining (NHEJ). This biological role is linked to pol mu's ability to promote NHEJ of ends with noncomplementary 3' overhangs, but questions remain regarding how it performs this role. W...

journal_title：Nucleic acids research

authors： Davis BJ,Havener JM,Ramsden DA

更新日期：2008-05-01 00:00:00

abstract：:Recombinase proteins assembled into helical filaments on DNA are believed to be the catalytic core of homologous recombination. The assembly, disassembly and dynamic rearrangements of this structure must drive the DNA strand exchange reactions of homologous recombination. The sensitivity of eukaryotic recombinase acti...

journal_title：Nucleic acids research

authors： Ristic D,Modesti M,van der Heijden T,van Noort J,Dekker C,Kanaar R,Wyman C

更新日期：2005-06-08 00:00:00

abstract：:Chemically modified antisense oligonucleotides (ASOs) with phosphorothioate (PS) linkages have been extensively studied as research and therapeutic agents. PS-ASOs can enter the cell and trigger cleavage of complementary RNA by RNase H1 even in the absence of transfection reagent. A number of cell surface proteins hav...

journal_title：Nucleic acids research

authors： Wang S,Allen N,Vickers TA,Revenko AS,Sun H,Liang XH,Crooke ST

更新日期：2018-04-20 00:00:00

abstract：:During protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) and in bacteria by the homologous protein EF-P. While a structure of EF-P bound to the 70S ribosome exists, structural insight into eIF-5A on ...

journal_title：Nucleic acids research

authors： Schmidt C,Becker T,Heuer A,Braunger K,Shanmuganathan V,Pech M,Berninghausen O,Wilson DN,Beckmann R

更新日期：2016-02-29 00:00:00

abstract：:Toxin-antitoxin systems are widespread in bacteria and archaea. They perform diverse functional roles, including the generation of persistence, maintenance of genetic loci and resistance to bacteriophages through abortive infection. Toxin-antitoxin systems have been divided into three types, depending on the nature of...

journal_title：Nucleic acids research

authors： Blower TR,Short FL,Rao F,Mizuguchi K,Pei XY,Fineran PC,Luisi BF,Salmond GP

更新日期：2012-07-01 00:00:00

abstract：:We have isolated an eight kilobase fragment of Bacillus subtilis DNA by specific integration and excision of a plasmid containing a sequence adjacent to ribosomal operon rrn O. The genetic locus of the cloned fragment was verified by linkage of the integrated vector to nearby genetic markers using both transduction an...

journal_title：Nucleic acids research

authors： Lampe MF,Bott KF

更新日期：1984-08-10 00:00:00

abstract：:Toxicogenomics focuses on assessing the safety of compounds using gene expression profiles. Gene expression signatures from large toxicogenomics databases are expected to perform better than small databases in identifying biomarkers for the prediction and evaluation of drug safety based on a compound's toxicological m...

journal_title：Nucleic acids research

authors： Igarashi Y,Nakatsu N,Yamashita T,Ono A,Ohno Y,Urushidani T,Yamada H

更新日期：2015-01-01 00:00:00

abstract：:DIAL (dihedral alignment) is a web server that provides public access to a new dynamic programming algorithm for pairwise 3D structural alignment of RNA. DIAL achieves quadratic time by performing an alignment that accounts for (i) pseudo-dihedral and/or dihedral angle similarity, (ii) nucleotide sequence similarity a...

journal_title：Nucleic acids research

authors： Ferrè F,Ponty Y,Lorenz WA,Clote P

更新日期：2007-07-01 00:00:00

abstract：:Molecular replacement (MR) is the method of choice for X-ray crystallography structure determination when structural homologues are available in the Protein Data Bank (PDB). Although the success rate of MR decreases sharply when the sequence similarity between template and target proteins drops below 35% identical res...

journal_title：Nucleic acids research

authors： Claude JB,Suhre K,Notredame C,Claverie JM,Abergel C

更新日期：2004-07-01 00:00:00

abstract：:From a sarkosyl-insoluble outer membrane fraction prepared from the Helicobacter pylori strain ATCC 43504, 19 proteins could be sequenced N-terminally by Edman degradation. Oligonucleotides were deduced and used for screening of a genomic library. From the isolated genes, five code for different members of a H.pylori ...

journal_title：Nucleic acids research

authors： Peck B,Ortkamp M,Diehl KD,Hundt E,Knapp B

更新日期：1999-08-15 00:00:00

abstract：:Pig Genomic Information System (PigGIS) is a web-based depository of pig (Sus scrofa) genomic learning mainly engineered for biomedical research to locate pig genes from their human homologs and position single nucleotide polymorphisms (SNPs) in different pig populations. It utilizes a variety of sequence data, includ...

journal_title：Nucleic acids research

authors： Ruan J,Guo Y,Li H,Hu Y,Song F,Huang X,Kristiensen K,Bolund L,Wang J

更新日期：2007-01-01 00:00:00

abstract：:The advent of RNA-sequencing and microarray technologies has led to rapid growth of transcriptome data generated for a wide range of organisms, under various cellular, organ and individual conditions. Since the number of possible combinations of intercellular and extracellular conditions is almost unlimited, catalogin...

journal_title：Nucleic acids research

authors： Obayashi T,Kagaya Y,Aoki Y,Tadaka S,Kinoshita K

更新日期：2019-01-08 00:00:00

abstract：:In order to investigate the possible role of RNA secondary structure in determining the efficiency and specificity of mRNA splicing, the structures of sequences at three acceptor splice sites in adenovirus were studied. Transcripts spanning intron-exon junctions were synthesized using SP6 RNA polymerase and analyzed u...

journal_title：Nucleic acids research

authors： Munroe SH

更新日期：1984-11-26 00:00:00

abstract：:Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent a...

journal_title：Nucleic acids research

authors： Nyerges Á,Csorgő B,Nagy I,Latinovics D,Szamecz B,Pósfai G,Pál C

更新日期：2014-04-01 00:00:00

abstract：:AR9 is a giant Bacillus subtilis phage whose uracil-containing double-stranded DNA genome encodes distant homologs of β and β' subunits of bacterial RNA polymerase (RNAP). The products of these genes are thought to assemble into two non-canonical multisubunit RNAPs - a virion RNAP (vRNAP) that is injected into the hos...

journal_title：Nucleic acids research

authors： Sokolova M,Borukhov S,Lavysh D,Artamonova T,Khodorkovskii M,Severinov K

更新日期：2017-06-02 00:00:00

abstract：:The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...

journal_title：Nucleic acids research

authors： Feng YX,Krupp G,Gross HJ

更新日期：1982-10-25 00:00:00

abstract：:DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes move processively along DNA. Here, an optical method has been dev...

journal_title：Nucleic acids research

authors： Fili N,Mashanov GI,Toseland CP,Batters C,Wallace MI,Yeeles JT,Dillingham MS,Webb MR,Molloy JE

更新日期：2010-07-01 00:00:00

abstract：:Differential display of mRNA is a simple, sensitive and powerful method to identify differentially expressed gene fragments. The main drawback of differential display is the lack of reproducibility and the inability to read and compare complex gels. This issue results from employing unoptimized primer combinations and...

journal_title：Nucleic acids research

authors： Malhotra K,Foltz L,Mahoney WC,Schueler PA

更新日期：1998-02-01 00:00:00

abstract：:The advent of jellyfish green fluorescent protein and its spectral variants, together with promising new fluorescent proteins from other classes of the Cnidarian phylum (coral and anemones), has greatly enhanced and promises to further boost the detection and localization of proteins in cell biology. It has been less ...

journal_title：Nucleic acids research

authors： Pederson T

更新日期：2001-03-01 00:00:00

abstract：:Well defined biomacromolecular patterns such as binding sites, catalytic sites, specific protein or nucleic acid sequences, etc. precisely modulate many important biological phenomena. We introduce PatternQuery, a web-based application designed for detection and fast extraction of such patterns. The application uses a...

journal_title：Nucleic acids research

authors： Sehnal D,Pravda L,Svobodová Vařeková R,Ionescu CM,Koča J

更新日期：2015-07-01 00:00:00

abstract：:The chemical shifts of the well-resolved ring nitrogen protons of uracil and a series of substituted uracils, including pseudouridine (5-ribosyl-uracil) and uridine (1-ribosyl-uracil) were determined using nuclear magnetic resonance (NMR). The observed chemical shifts suggest the existence of an atypical syn conformat...

journal_title：Nucleic acids research

authors： Hurd RE,Reid BR

更新日期：1977-08-01 00:00:00

abstract：:The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible m...

journal_title：Nucleic acids research

authors： Amirikyan BR,Vologodskii AV,Lyubchenko YuL

更新日期：1981-10-24 00:00:00

abstract：:T7 RNA polymerase promoters consist of a highly conserved 23 base-pair sequence that spans the site of the initiation of transcription (+1) and extends from -17 to +6. To determine the bases within the T7 consensus promoter that are essential for promoter function a library of mutant T7 promoters was constructed, and ...

journal_title：Nucleic acids research

authors： Ikeda RA,Ligman CM,Warshamana S

更新日期：1992-05-25 00:00:00

abstract：:A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two promi...

journal_title：Nucleic acids research

authors： Toloczyki C,Feix G

更新日期：1986-06-25 00:00:00

abstract：:In this article, we characterize histone demethylase activity of the entire family of JmjC+N proteins of Drosophila melanogaster. Our results show that Lid (little imaginal discs), which is structurally homologous to JARID1, demethylates H3K4me3. However, contrary to what would be inferred from its demethylase activit...

journal_title：Nucleic acids research

authors： Lloret-Llinares M,Carré C,Vaquero A,de Olano N,Azorín F

更新日期：2008-05-01 00:00:00

abstract：:We describe snap-to-it probes, a novel probe technology to enhance the hybridization specificity of natural and unnatural nucleic acid oligomers using a simple and readily introduced structural motif. Snap-to-it probes were prepared from peptide nucleic acid (PNA) oligomers by modifying each terminus with a coordinati...

journal_title：Nucleic acids research

authors： Morgan JR,Lyon RP,Maeda DY,Zebala JA

更新日期：2008-06-01 00:00:00