Abstract:
:A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two prominent S1 mapping signals. The sequence of a typical repeat unit is compared to a corresponding 130 bp long wheat repeat unit. The possible functional relevance of the repeat units is discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Toloczyki C,Feix Gdoi
10.1093/nar/14.12.4969subject
Has Abstractpub_date
1986-06-25 00:00:00pages
4969-86issue
12eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract::Using a combination of mathematical modelling, statistical simulation and large-scale data analysis we study the properties of linear regulatory chains (LRCs) within gene regulatory networks (GRNs). Our modelling indicates that downstream genes embedded within LRCs are highly insulated from the variation in expression...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx485
更新日期:2017-07-07 00:00:00
abstract::Poly(ADP-ribosyl)ation is involved in numerous bio-logical processes including DNA repair, transcription and cell death. Cellular levels of poly(ADP-ribose) (PAR) are regulated by PAR polymerases (PARPs) and the degrading enzyme PAR glycohydrolase (PARG), controlling the cell fate decision between life and death in re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku505
更新日期:2014-07-01 00:00:00
abstract::Optogenetic control of CRISPR-Cas9 systems has significantly improved our ability to perform genome perturbations in living cells with high precision in time and space. As new Cas orthologues with advantageous properties are rapidly being discovered and engineered, the need for straightforward strategies to control th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1198
更新日期:2020-12-16 00:00:00
abstract::Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa195
更新日期:2020-06-04 00:00:00
abstract::We have investigated loop-induced structural perturbation of the stem structure in hairpins d(GAATTCXnGAATTC) (X = A, T and n = 3, 4, 5 and 6) that contain an EcoRI restriction site in close proximity to the hairpin loop. Oligonucleotides containing either a T3 or a A3 loop were not hydrolyzed by the restriction enzym...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.6.1489
更新日期:1990-03-25 00:00:00
abstract::Controller (C) proteins regulate the timing of the expression of restriction and modification (R-M) genes through a combination of positive and negative feedback circuits. A single dimer bound to the operator switches on transcription of the C-gene and the endonuclease gene; at higher concentrations, a second dimer bo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn448
更新日期:2008-08-01 00:00:00
abstract::Synthetic genetic sensors and circuits enable programmable control over timing and conditions of gene expression and, as a result, are increasingly incorporated into the control of complex and multi-gene pathways. Size and complexity of genetic circuits are growing, but stay limited by a shortage of regulatory parts t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky010
更新日期:2018-02-28 00:00:00
abstract::KUPS (The University of Kansas Proteomics Service) provides high-quality protein-protein interaction (PPI) data for researchers developing and evaluating computational models for predicting PPIs by allowing users to construct ready-to-use data sets of interacting protein pairs (IPPs), non-interacting protein pairs (NI...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq943
更新日期:2011-01-01 00:00:00
abstract::The Superimposé webserver performs structural similarity searches with a preference towards 3D structure-based methods. Similarities can be detected between small molecules (e.g. drugs), parts of large structures (e.g. binding sites of proteins) and entire proteins. For this purpose, a number of algorithms were implem...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn285
更新日期:2008-07-01 00:00:00
abstract::Expression from the K. pneumoniae nifLA promoter is oxygen sensitive and is also inhibited by the DNA gyrase inhibitor coumermycin A1 under anaerobic growth conditions. The activity of this promoter was found to be highly sensitive to changes in DNA topology in vitro. Transcription was completely dependent on negative...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.21.9933
更新日期:1988-11-11 00:00:00
abstract::More than one-third of the RAG1 protein can be truncated from the N-terminus with only subtle effects on the products of V(D)J recombination in vitro or in a mouse. What, then, is the function of the N-terminal domain? We believe it to be regulatory. We determined, several years ago, that an included RING motif could ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp192
更新日期:2009-06-01 00:00:00
abstract::A new approach has been developed for the rapid fragmentation and fractionation of DNA into a size suitable for shotgun cloning and sequencing. The restriction endonuclease CviJI normally cleaves the recognition sequence PuGCPy between the G and C to leave blunt ends. Atypical reaction conditions which alter the speci...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.14.3753
更新日期:1992-07-25 00:00:00
abstract::Mammalian mitochondrial ribosomes evolved from bacterial ribosomes by reduction of ribosomal RNAs, increase of ribosomal protein content, and loss of guanine nucleotides. Guanine is the base most sensitive to oxidative damage. By systematically comparing high-quality, small ribosomal subunit RNA sequence alignments an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky762
更新日期:2018-11-16 00:00:00
abstract::The nucleoside analogs 1-(2'-deoxy-beta-D-ribofuranosyl)- 3-nitropyrrole (9), 1-(2'-deoxy-beta-D-ribofuranosyl)-4-nitropyrazole (10), 1-(2'-deoxy-beta-D-ribofuranosyl)-4-nitroimidazole (11) and 1-(2'-deoxy-beta-D-ribofuranosyl)-5-nitroindole (21) were incorporated into the oligonucleotide 5'-d(CGCXAATTYGCG)-3'in the f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.10.1935
更新日期:1997-05-15 00:00:00
abstract::Using cyanoethyldiisopropylamino phosphoramidite chemistry, four oligonucleotides constituting a part of the sequence of the initiator t-RNA from B. subtilis were synthesized. For the protection of the exocyclic amino functions of bases, phenoxyacetyl group was used for adenine and guanine, and acetyl group was prefer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.18.7381
更新日期:1989-09-25 00:00:00
abstract::Unrepaired DNA damage may arrest ongoing replication forks, potentially resulting in fork collapse, increased mutagenesis and genomic instability. Replication through DNA lesions depends on mono- and polyubiquitylation of proliferating cell nuclear antigen (PCNA), which enable translesion synthesis (TLS) and template ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks850
更新日期:2012-11-01 00:00:00
abstract::Chemically modified siRNAs are expected to have resistance toward nuclease degradation and good thermal stability in duplex formation for in vivo applications. We have recently found that 2'-OMe-4'-thioRNA, a hybrid chemical modification based on 2'-OMeRNA and 4'-thioRNA, has high hybridization affinity for complement...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks204
更新日期:2012-07-01 00:00:00
abstract::We identify a novel activity of the RarA (also MgsA) protein of Escherichia coli, demonstrating that this protein functions at DNA ends to generate flaps. A AAA+ ATPase in the clamp loader clade, RarA protein is part of a highly conserved family of DNA metabolism proteins. We demonstrate that RarA binds to double-stra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1322
更新日期:2017-03-17 00:00:00
abstract::DNA transposon-based vectors have emerged as gene vehicles with a wide biomedical and therapeutic potential. So far, genomic insertion of such vectors has relied on the co-delivery of genetic material encoding the gene-inserting transposase protein, raising concerns related to persistent expression, insertional mutage...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1163
更新日期:2014-02-01 00:00:00
abstract::The Structural Classification of Proteins (SCOP) database is a comprehensive ordering of all proteins of known structure, according to their evolutionary and structural relationships. Protein domains in SCOP are hierarchically classified into families, superfamilies, folds and classes. The continual accumulation of se...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh039
更新日期:2004-01-01 00:00:00
abstract::Archival formalin-fixed, paraffin-embedded and ethanol-fixed tissues represent a potentially invaluable resource for gene expression analysis, as they are the most widely available material for studies of human disease. Little data are available evaluating whether RNA obtained from fixed (archival) tissues could produ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.2.e4
更新日期:2002-01-15 00:00:00
abstract::Biological networks are a topic of great current interest, particularly with the publication of a number of large genome-wide interaction datasets. They are globally characterized by a variety of graph-theoretic statistics, such as the degree distribution, clustering coefficient, characteristic path length and diamete...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh164
更新日期:2004-01-14 00:00:00
abstract::Basic region-leucine zipper (B-ZIP) proteins are a class of dimeric sequence-specific DNA-binding proteins unique to eukaryotes. We have identified 67 B-ZIP proteins in the Arabidopsis thaliana genome. No A.thaliana B-ZIP domains are homologous with any Homo sapiens B-ZIP domains. Here, we predict the dimerization spe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh653
更新日期:2004-06-29 00:00:00
abstract::In the brome mosaic virus (BMV) virion, the coat protein (CP) selectively contacts the RNA motifs that regulate translation and RNA replication (Hoover et al., 2016. J. Virol. 90, 7748). We hypothesize that the unstructured N-terminal arm (NTA) of the BMV CP can specifically recognize RNA motifs. Using ion mobility sp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1087
更新日期:2018-01-09 00:00:00
abstract::Recent studies show that the region of DNA brought into close proximity to the fetal gamma-globin genes in deletional forms of hereditary persistence of fetal hemoglobin (HPFH) is selectively hypomethylated (and presumably active) in normal erythroid tissue. This region is normally located approximately 100 kilobases ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.13.5169
更新日期:1987-07-10 00:00:00
abstract::The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silence...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr551
更新日期:2011-10-01 00:00:00
abstract::We have determined the three-dimensional structure of a non-selfcomplementary nonanucleotide duplex which contains an abasic (apyrimidinic) site in the centre, i.e. a deoxyribose residue opposite an adenosine. The majority of the base and sugar proton resonances were assigned by NOESY, COSY and 2DQF spectra in D2O and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.19.8003
更新日期:1987-10-12 00:00:00
abstract::The nucleotide sequence of the Anacystis nidulans 23S rRNA gene, including the 5'- and 3'-flanking regions has been determined. The gene is 2876 nucleotides long and shows higher primary sequence homology to the 23S rRNAs of plastids (84.5%) than to that of E. coli (79%). The predicted rRNA transcript also shares many...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.7.3373
更新日期:1984-04-11 00:00:00
abstract::Accumulation of replicative intermediates of the bacteriophage phi X174 was observed in E. coli C infected cells when phage DNA methylation has been inhibited by nicotinamide or when cells were infected with a temperature-sensitive mutant in gene A. Analysis of the accumulating replicative intermediates by electron mi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.10.3483
更新日期:1977-10-01 00:00:00
abstract::We found previously that the Chlamydomonas HSP70A promoter counteracts transcriptional silencing of downstream promoters in a transgene setting. To elucidate the underlying mechanisms, we analyzed chromatin state and transgene expression in transformants containing HSP70A-RBCS2-ble (AR-ble) constructs harboring deleti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt224
更新日期:2013-05-01 00:00:00