Abstract:
:Expression from the K. pneumoniae nifLA promoter is oxygen sensitive and is also inhibited by the DNA gyrase inhibitor coumermycin A1 under anaerobic growth conditions. The activity of this promoter was found to be highly sensitive to changes in DNA topology in vitro. Transcription was completely dependent on negative supercoiling at physiological salt concentrations although transcription from linear or fully relaxed closed circular templates was detectable at KCl concentrations lower than 50 mM. These observations suggest that aerobic regulation of nif transcription may be mediated through the level of DNA supercoiling.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Dixon RA,Henderson NC,Austin Sdoi
10.1093/nar/16.21.9933subject
Has Abstractpub_date
1988-11-11 00:00:00pages
9933-46issue
21eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::Chlorella virus PBCV-1 DNA ligase seals nicked DNA substrates consisting of a 5'-phosphate-terminated strand and a 3'-hydroxyl-terminated strand annealed to a bridging DNA template strand. The enzyme discriminates at the DNA binding step between substrates containing a 5'-phosphate versus a 5'-hydroxyl at the nick. Mu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.2.525
更新日期:1998-01-15 00:00:00
abstract::SynergyFinder (https://synergyfinder.fimm.fi) is a stand-alone web-application for interactive analysis and visualization of drug combination screening data. Since its first release in 2017, SynergyFinder has become a widely used web-tool both for the discovery of novel synergistic drug combinations in pre-clinical mo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa216
更新日期:2020-07-02 00:00:00
abstract::High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed. In this work, we have developed and implemented a set of synthetic 16S rRNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw984
更新日期:2017-02-28 00:00:00
abstract::We have developed a comprehensive resource devoted to biologists wanting to optimize the use of gene trap clones in their experiments. We have processed 300 602 such clones from both public and private projects to generate 28,199 'UniTraps', i.e. distinct collections of unambiguous insertions at the same subgenic regi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm825
更新日期:2008-01-01 00:00:00
abstract::Treatment of NIH 3T3 cells with trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), resulted in a dose-dependent increase in transcription from a rDNA reporter and from endogenous rRNA genes. Chromatin immunoprecipitation using anti-acetyl-histone H4 antibodies demonstrated a direct effect of TSA on the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.20.4114
更新日期:2001-10-15 00:00:00
abstract::Abasic sites are common DNA lesions resulting from spontaneous depurination and excision of damaged nucleobases by DNA repair enzymes. However, the influence of the local sequence context on the structure of the abasic site and ultimately, its recognition and repair, remains elusive. In the present study, duplex DNAs ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm622
更新日期:2008-01-01 00:00:00
abstract::A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.5.1081
更新日期:1980-03-11 00:00:00
abstract::Invariance of temporal order of genome replication in eukaryotic cells and its correlation with gene activity has been well-documented. However, recent data suggest a relax control of replication timing. To evaluate replication schedule accuracy, we detailed the replicational organization of the developmentally regula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm586
更新日期:2007-01-01 00:00:00
abstract::The nucleotide sequence of the promoter region and the first five genes of the atp (or unc) operon of Escherichia coli has been determined. The first proposed gene in the operon contains four AUA codons and may be poorly expressed; it encodes a basic but yet hydrophobic protein which could function as a pilot protein ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.16.3919
更新日期:1981-08-25 00:00:00
abstract::Targeted modulation of gene expression represents a valuable approach to understand the mechanisms governing gene regulation. In a therapeutic context, it can be exploited to selectively modify the aberrant expression of a disease-causing gene or to provide the target cells with a new function. Here, we have establish...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky171
更新日期:2018-05-18 00:00:00
abstract::The targeted adduction of aflatoxin B1- exo -8,9-epoxide (AFB1- exo -8,9-epoxide) to a specific guanine within an oligodeoxyribonucleotide containing multiple guanines was achieved using a DNA triplex to control sequence selectivity. The oligodeoxyribonucleotide d(AGAGAAGATTTTCTTCTCTTTTTTTTCTCTT), designated '3G', spo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.4.1070
更新日期:1998-02-15 00:00:00
abstract::MicroArray Gene expression and Network Evaluation Toolkit (MAGNET) is a web-based application that provides tools to generate and score both protein-protein interaction networks and coexpression networks. MAGNET integrates user-provided experimental measurements with high-throughput proteomic datasets, generating weig...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks526
更新日期:2012-07-01 00:00:00
abstract::This laboratory has established in previous studies that Pmel 17, a gene expressed specifically in melanocytes, maps near the silver coat color locus (si/si) on mouse chromosome 10. In the current study, we have focused on determining whether or not the si allele carries a mutation in Pmel 17. Pmel 17 cDNA clones, iso...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.1.154
更新日期:1995-01-11 00:00:00
abstract::Differences between species have been suggested to largely reside in the network of connections among the genes. Nevertheless, the rate at which these connections evolve has not been properly quantified. Here, we measure the extent to which co-regulation between pairs of genes is conserved over large phylogenetic dist...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh815
更新日期:2004-09-07 00:00:00
abstract::Onconase, a cytotoxic ribonuclease from Rana pipiens, possesses pyroglutamate (Pyr) at the N-terminus and has a substrate preference for uridine-guanine (UG). To identify residues responsible for onconase's cytotoxicity, we cloned the rpr gene from genomic DNA and expressed it in Escherichia coli BL21(DE3). The recomb...
journal_title:Nucleic acids research
pub_type: 评论,杂志文章
doi:10.1093/nar/gkg746
更新日期:2003-09-15 00:00:00
abstract::We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.10.e45
更新日期:2002-05-15 00:00:00
abstract::Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.16.3121
更新日期:1996-08-15 00:00:00
abstract::UMSBP is a CCHC-type zinc finger protein, which functions during replication initiation of kinetoplast DNA minicircles and the segregation of kinetoplast DNA networks. Interactions of UMSBP with origin sequences, as well as the protein oligomerization, are affected by its redox state. Reduction yields UMSBP monomers a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn927
更新日期:2009-01-01 00:00:00
abstract::Group II introns are self-splicing RNAs and retroelements found in bacteria and lower eukaryotic organelles. During the past several years, they have been uncovered in surprising numbers in bacteria due to the genome sequencing projects; however, most of the newly sequenced introns are not correctly identified. We hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg049
更新日期:2003-01-01 00:00:00
abstract::The Class 3 aldehyde dehydrogenase gene (ALDH-3) is differentially expressed. Expression is either constitutive or xenobiotic inducible via an aromatic hydrocarbon (Ah) receptor-mediated pathway, depending upon the tissue. A series of studies were performed to examine the regulation of rat ALDH-3 basal expression. DNa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.21.4185
更新日期:1996-11-01 00:00:00
abstract::We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly purified 5-MeC-DNA glycosylase complex were identified by Nanoelectrospray ionisation mass spectrometry to be identical to the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.16.3245
更新日期:1999-08-15 00:00:00
abstract::Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ell...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.8.2089
更新日期:1976-08-01 00:00:00
abstract::The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing e...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng045
更新日期:2003-04-15 00:00:00
abstract::IMGT, the international ImMunoGeneTics database (http://imgt.cines. fr:8104 ), is a high-quality integrated database specialising in Immunoglobulins (Ig), T cell Receptors (TcR) and Major Histocompatibility Complex (MHC) molecules of all vertebrate species, created in 1989 by Marie-Paule Lefranc, Université Montpellie...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.219
更新日期:2000-01-01 00:00:00
abstract::Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.22.9255
更新日期:1987-11-25 00:00:00
abstract::After ionising radiation double-strand breaks (dsb) are lethal if not repaired or misrepaired. Cell killing is greatly enhanced by hyperthermia and it is questioned here whether heat not only affects dsb repair capacity but also fidelity in a chromosomal context. dsb repair experiments were designed so as to mainly sc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.9.1960
更新日期:2001-05-01 00:00:00
abstract::The ProtoNet site provides an automatic hierarchical clustering of the SWISS-PROT protein database. The clustering is based on an all-against-all BLAST similarity search. The similarities' E-score is used to perform a continuous bottom-up clustering process by applying alternative rules for merging clusters. The outco...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg096
更新日期:2003-01-01 00:00:00
abstract::Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.13.2806
更新日期:1999-07-01 00:00:00
abstract::The small subunit (SSU) processome is a large ribonucleoprotein that is required for maturation of the 18S rRNA of the ribosome. Recently, a missense mutation in the C-terminus of an SSU processome protein, Utp4/Cirhin, was reported to cause North American Indian childhood cirrhosis (NAIC). In this study, we use Sacch...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq185
更新日期:2010-08-01 00:00:00
abstract::Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. The CORUM (http://mips.gsf.de/genre/proj/corum/index.html) database is a collection of experimentally verified mammalian protein complexes. Information is manually derived by critical reading of the scient...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm936
更新日期:2008-01-01 00:00:00