DNA supercoiling and aerobic regulation of transcription from the Klebsiella pneumoniae nifLA promoter.

abstract：:The secondary structure of V4, the largest variable area of eukaryotic small subunit ribosomal RNA, was re-examined by comparative analysis of 3253 nucleotide sequences distributed over the animal, plant and fungal kingdoms and a diverse set of protist taxa. An extensive search for compensating base pair substitutions...

journal_title：Nucleic acids research

authors： Wuyts J,De Rijk P,Van de Peer Y,Pison G,Rousseeuw P,De Wachter R

更新日期：2000-12-01 00:00:00

abstract：:EcoRI digestion of total mouse DNA yields a prominant 1.3 kb fragment amounting to between 1 and 2% of the mouse genome. The majority of the 1.3 kb EcoRI fragments have a single Bg1II site 800 bp from one end. This EcoRI-Bg1II sequence family shows HindIII and HaeIII sequence heterogeneity. We have cloned representati...

journal_title：Nucleic acids research

authors： Heller R,Arnheim N

更新日期：1980-11-11 00:00:00

abstract：:Tetrahymena thermophila mitochondrial DNA is a linear molecule with two tRNAs, large subunit beta (LSU beta) rRNA (21S rRNA) and LSU alpha rRNA (5.8S-like RNA) encoded near each terminus. The DNA sequence of approximately 550 bp of this region was determined in six species of Tetrahymena. In three species the LSU beta...

journal_title：Nucleic acids research

authors： Morin GB,Cech TR

更新日期：1988-01-11 00:00:00

abstract：:The detailed principles of the hierarchical folding of eukaryotic chromosomes have been revealed during the last two decades. Along with structures composing three-dimensional (3D) genome organization (chromatin compartments, topologically associating domains, chromatin loops, etc.), the molecular mechanisms that are ...

journal_title：Nucleic acids research

authors： Kantidze OL,Razin SV

更新日期：2020-05-21 00:00:00

abstract：:Although telomeres are not recognized as double-strand breaks (DSBs), some DSB repair proteins are present at telomeres and are required for telomere maintenance. To learn more about the telomeric function of proteins from the homologous recombination (HR) and non-homologous end joining pathways (NHEJ), we have screen...

journal_title：Nucleic acids research

authors： Wei C,Skopp R,Takata M,Takeda S,Price CM

更新日期：2002-07-01 00:00:00

abstract：:Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...

journal_title：Nucleic acids research

authors： Zamdborg L,Ma P

更新日期：2009-09-01 00:00:00

abstract：:The structure-specific Mus81-Eme1/Mms4 endonuclease contributes importantly to DNA repair and genome integrity maintenance. Here, using budding yeast, we have studied its function and regulation during the cellular response to DNA damage and show that this endonuclease is necessary for successful chromosome replicatio...

journal_title：Nucleic acids research

authors： Saugar I,Vázquez MV,Gallo-Fernández M,Ortiz-Bazán MÁ,Segurado M,Calzada A,Tercero JA

更新日期：2013-10-01 00:00:00

abstract：:Alternative splicing is widespread in mammalian gene expression, and variant splice patterns are often specific to different stages of development, particular tissues or a disease state. There is a need to systematically collect data on alternatively spliced exons, introns and splice isoforms, and to annotate this dat...

journal_title：Nucleic acids research

authors： Thanaraj TA,Stamm S,Clark F,Riethoven JJ,Le Texier V,Muilu J

更新日期：2004-01-01 00:00:00

abstract：:The highly charged DNA chain may be either in an extended conformation, the coil, or condensed into a highly dense and ordered structure, the toroid. The transition, also called collapse of the chain, can be triggered in different ways, for example by changing the ionic conditions of the solution. We observe individua...

journal_title：Nucleic acids research

authors： Sung B,Leforestier A,Livolant F

更新日期：2016-02-18 00:00:00

abstract：:Identification of components essential to chromosome structure and behaviour remains a vibrant area of study. We have previously shown that invadolysin is essential in Drosophila, with roles in cell division and cell migration. Mitotic chromosomes are hypercondensed in length, but display an aberrant fuzzy appearance....

journal_title：Nucleic acids research

authors： Rao SG,Janiszewski MM,Duca E,Nelson B,Abhinav K,Panagakou I,Vass S,Heck MM

更新日期：2015-04-20 00:00:00

abstract：:A very high rate of multidrug resistance (MDR) seen among Gram-negative bacteria such as Escherichia, Klebsiella, Salmonella, Shigella, etc. is a major threat to public health and safety. One of the major virulent determinants of Gram-negative bacteria is capsular polysaccharide or K antigen located on the bacterial o...

journal_title：Nucleic acids research

authors： Kunduru BR,Nair SA,Rathinavelan T

更新日期：2016-01-04 00:00:00

abstract：:A novel, site-specific, DNA backbone S-modification (phosphorothioation) has been discovered, but its in vivo function(s) have remained obscure. Here, we report that the enteropathogenic Salmonella enterica serovar Cerro 87, which possesses S-modified DNA, restricts DNA isolated from Escherichia coli, while protecting...

journal_title：Nucleic acids research

authors： Xu T,Yao F,Zhou X,Deng Z,You D

更新日期：2010-11-01 00:00:00

abstract：:The VS ribozyme trans-cleavage substrate interacts with the catalytic RNA via tertiary interactions. To study the role of phosphate groups in the ribozyme-substrate interaction, 18 modified substrates were synthesized, where an epimeric phosphorothioate replaces one of the phosphate diester linkages. Sites in the stem...

journal_title：Nucleic acids research

authors： Kovacheva YS,Tzokov SB,Murray IA,Grasby JA

更新日期：2004-12-01 00:00:00

abstract：:Plant specialized metabolites are chemically highly diverse, play key roles in host-microbe interactions, have important nutritional value in crops and are frequently applied as medicines. It has recently become clear that plant biosynthetic pathway-encoding genes are sometimes densely clustered in specific genomic lo...

journal_title：Nucleic acids research

authors： Kautsar SA,Suarez Duran HG,Blin K,Osbourn A,Medema MH

更新日期：2017-07-03 00:00:00

abstract：:The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco p...

journal_title：Nucleic acids research

authors： Dean C,Jones J,Favreau M,Dunsmuir P,Bedbrook J

更新日期：1988-10-11 00:00:00

abstract：:We have determined the nucleotide sequence at the 3' end of the vesicular stomatitis virus (VSV) polymerase or L gene and compared it to that obtained from a defective interfering particle (DI) RNA generated by this virus. The latter (DI 0.50) contains a large internal deletion within this gene. The deletion begins ex...

journal_title：Nucleic acids research

authors： De BK,Perrault J

更新日期：1982-11-11 00:00:00

abstract：:The iProClass database provides comprehensive, value-added descriptions of proteins and serves as a framework for data integration in a distributed networking environment. The protein information in iProClass includes family relationships as well as structural and functional classifications and features. The current v...

journal_title：Nucleic acids research

authors： Huang H,Barker WC,Chen Y,Wu CH

更新日期：2003-01-01 00:00:00

abstract：:Recently, a few Na+-specific RNA-cleaving DNAzymes were reported, where nucleobases are likely to play critical roles in catalysis. The NaA43 and NaH1 DNAzymes share the same 16-nt Na+-binding motif, but differ in one or two nucleotides in a small catalytic loop. Nevertheless, they display an opposite pH-dependency, i...

journal_title：Nucleic acids research

authors： Ma L,Kartik S,Liu B,Liu J

更新日期：2019-09-05 00:00:00

abstract：:The MalaCards human disease database (http://www.malacards.org/) is an integrated compendium of annotated diseases mined from 68 data sources. MalaCards has a web card for each of ∼20 000 disease entries, in six global categories. It portrays a broad array of annotation topics in 15 sections, including Summaries, Symp...

journal_title：Nucleic acids research

authors： Rappaport N,Twik M,Plaschkes I,Nudel R,Iny Stein T,Levitt J,Gershoni M,Morrey CP,Safran M,Lancet D

更新日期：2017-01-04 00:00:00

abstract：:The DNA repair enzyme human uracil DNA glycosylase (UNG) scans short stretches of genomic DNA and captures rare uracil bases as they transiently emerge from the DNA duplex via spontaneous base pair breathing motions. The process of DNA scanning requires that the enzyme transiently loosen its grip on DNA to allow stoch...

journal_title：Nucleic acids research

authors： Friedman JI,Majumdar A,Stivers JT

更新日期：2009-06-01 00:00:00

abstract：:Targeted modulation of gene expression represents a valuable approach to understand the mechanisms governing gene regulation. In a therapeutic context, it can be exploited to selectively modify the aberrant expression of a disease-causing gene or to provide the target cells with a new function. Here, we have establish...

journal_title：Nucleic acids research

authors： Mlambo T,Nitsch S,Hildenbeutel M,Romito M,Müller M,Bossen C,Diederichs S,Cornu TI,Cathomen T,Mussolino C

更新日期：2018-05-18 00:00:00

abstract：:RegulonDB, first published 20 years ago, is a comprehensive electronic resource about regulation of transcription initiation of Escherichia coli K-12 with decades of knowledge from classic molecular biology experiments, and recently also from high-throughput genomic methodologies. We curated the literature to keep Reg...

journal_title：Nucleic acids research

authors： Santos-Zavaleta A,Salgado H,Gama-Castro S,Sánchez-Pérez M,Gómez-Romero L,Ledezma-Tejeida D,García-Sotelo JS,Alquicira-Hernández K,Muñiz-Rascado LJ,Peña-Loredo P,Ishida-Gutiérrez C,Velázquez-Ramírez DA,Del Moral-Chávez V,Bonavid

更新日期：2019-01-08 00:00:00

abstract：:The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and mai...

journal_title：Nucleic acids research

authors： Agostini F,Cirillo D,Bolognesi B,Tartaglia GG

更新日期：2013-01-07 00:00:00

abstract：:Human Silent Information Regulator Type 1 (SIRT1) is an NAD(+)-dependent deacetylase protein which is an intermediary of cellular metabolism in gene silencing and aging. SIRT1 has been extensively investigated and shown to delay senescence; however, less is known about the regulation of SIRT1 during aging. In this stu...

journal_title：Nucleic acids research

authors： Han L,Zhou R,Niu J,McNutt MA,Wang P,Tong T

更新日期：2010-11-01 00:00:00

abstract：:Gene trap mutagenesis of mouse embryonic stem cells generates random loss-of-function mutations, which can be identified by a sequence tag and can often report the endogenous expression of the mutated gene. The Centre for Modeling Human Disease is performing expression- and sequence-based screens of gene trap insertio...

journal_title：Nucleic acids research

authors： To C,Epp T,Reid T,Lan Q,Yu M,Li CY,Ohishi M,Hant P,Tsao N,Casallo G,Rossant J,Osborne LR,Stanford WL

更新日期：2004-01-01 00:00:00

abstract：:Simian cells have been transformed with SV40 origin-defective recombinant plasmids containing the tsA209 T-antigen gene. These plasmids contain deletions of either 5 or 52 nucleotides that include the BglI site at the SV40 ori, are defective for replication in COS-1 cells but retain a functional SV40 early promoter. T...

journal_title：Nucleic acids research

authors： Portela A,de la Luna S,Melero JA,Vara J,Jiménez A,Ortín J

更新日期：1985-11-25 00:00:00

abstract：:We have compiled the DNA sequence data for E. coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the fifth listing replacing and increasing the former listings substantially. However, in order to save space this printed version contains...

journal_title：Nucleic acids research

authors： Kröger M,Wahl R,Rice P

更新日期：1993-07-01 00:00:00

abstract：:In phytohemagglutinin stimulated human lymphocytes the time relationship was determined between induction of the parameters mentioned. The results indicate that the induction occurred in a specific sequence. Thus, a simultaneous increase in the activity of DNA polymerase and thymidinekinase occurred after 15 h of incu...

journal_title：Nucleic acids research

authors： Tyrsted G,Munch-Petersen B

更新日期：1977-08-01 00:00:00

abstract：:Polyaromatic molecules with amino chain substituents, upon binding with DNA, selectively catalyze exchange of the A.T base pair protons with bulk water protons. The amine-catalyzed exchange is mediated by compounds which are A.T and G.C base sequence specific, intercalators, and outside binders. A mechanism for the se...

journal_title：Nucleic acids research

authors： Strekowski L,Watson RA,Wilson WD

更新日期：1987-10-26 00:00:00

abstract：:The structure and organisation of the human N-ras gene has been determined by analysing cDNA clones derived from the two main mRNA transcripts. One clone in particular is 4.1 Kb long and originates from the larger (4.3 Kb) message. Sequence analysis of this clone has revealed that the N-ras gene consists of seven exon...

journal_title：Nucleic acids research

authors： Hall A,Brown R

更新日期：1985-07-25 00:00:00