Abstract:
:Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, in situ, this increase appears to be due to an increase in the number of cells expressing the transfected DNA. The stimulation in levels of expression is not correlated to any specific DNA sequence, nor does it occur if cells are exposed to hormone post-transfection.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sinclair JH,Bryant LAdoi
10.1093/nar/15.22.9255subject
Has Abstractpub_date
1987-11-25 00:00:00pages
9255-61issue
22eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract::OrthoDB is a comprehensive catalog of orthologs, genes inherited by extant species from a single gene in their last common ancestor. In 2016 OrthoDB reached its 9th release, growing to over 22 million genes from over 5000 species, now adding plants, archaea and viruses. In this update we focused on usability of this f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1119
更新日期:2017-01-04 00:00:00
abstract::Serine integrases are emerging as core tools in synthetic biology and have applications in biotechnology and genome engineering. We have designed a split-intein serine integrase-based system with potential for regulation of site-specific recombination events at the protein level in vivo. The ϕC31 integrase was split i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz936
更新日期:2019-12-02 00:00:00
abstract::T1DBase (http://www.t1dbase.org) is web platform, which supports the type 1 diabetes (T1D) community. It integrates genetic, genomic and expression data relevant to T1D research across mouse, rat and human and presents this to the user as a set of web pages and tools. This update describes the incorporation of new dat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq912
更新日期:2011-01-01 00:00:00
abstract::Overexpression of the flap endonuclease FEN1 has been observed in a variety of cancer types and is a marker for poor prognosis. To better understand the cellular consequences of FEN1 overexpression we utilized a model of its Saccharomyces cerevisiae homolog, RAD27. In this system, we discovered that flap endonuclease ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky313
更新日期:2018-06-20 00:00:00
abstract::Homeodomains appear to be one of the most frequently employed DNA-binding domains in a superfamily of transacting factors. It is likely that during evolution several sub-types of homeodomain have evolved from a common ancestral domain, resulting in distinct but closely related DNA-binding preferences. Here we describe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.7.1202
更新日期:1994-04-11 00:00:00
abstract::The excision of specific DNA sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. Furthermore, manipulation of potential control elements in the context of a single integration site mitigates against insertion site i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng148
更新日期:2003-11-15 00:00:00
abstract::Adenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5' splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh536
更新日期:2004-04-15 00:00:00
abstract::U1-snRNA is an integral part of the U1 ribonucleoprotein pivotal for pre-mRNA splicing. Toll-like receptor (TLR) signaling has recently been associated with immunoregulatory capacities of U1-snRNA. Using lung A549 epithelial/carcinoma cells, we report for the first time on interferon regulatory factor (IRF)-3 activati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp525
更新日期:2009-08-01 00:00:00
abstract::Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specifi...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2001-05-15 00:00:00
abstract::An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its applicat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.9.2963
更新日期:1979-07-11 00:00:00
abstract::OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays. The availability of probe sequences, associated with custom microarray fabrication services offered by many companies and co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn761
更新日期:2009-01-01 00:00:00
abstract::Properties of a mutant bacteriophage T2 DNA [N:(6)-adenine] methyltransferase (T2 Dam MTase) have been investigated for its potential utilization in RecA-assisted restriction endonuclease (RARE) cleavage. Steady-state kinetic analyses with oligonucleotide duplexes revealed that, compared to wild-type T4 Dam, both wild...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.7.1484
更新日期:2001-04-01 00:00:00
abstract::Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq456
更新日期:2010-10-01 00:00:00
abstract::Three Rat1/Xrn2 homologues exist in Arabidopsis thaliana: nuclear AtXRN2 and AtXRN3, and cytoplasmic AtXRN4. The latter has a role in degrading 3' products of miRNA-mediated mRNA cleavage, whereas all three proteins act as endogenous post-transcriptional gene silencing suppressors. Here we show that, similar to yeast ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq172
更新日期:2010-07-01 00:00:00
abstract::Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFbeta superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFbeta on Pax6 expression was studied in t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1105
更新日期:2007-01-01 00:00:00
abstract::Methylation of miRNAs at the 2'-hydroxyl group on the ribose at 3'-end (2'-O-methylation, 2'Ome) is critical for miRNA function in plants and Drosophila. Whether this methylation phenomenon exists for mammalian miRNA remains unknown. Through LC-MS/MS analysis, we discover that majority of miR-21-5p isolated from human...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa504
更新日期:2020-07-27 00:00:00
abstract::Transcription factor (TF) and microRNA (miRNA) are two crucial trans-regulatory factors that coordinately control gene expression. Understanding the impacts of these two factors on the rate of protein sequence evolution is of great importance in evolutionary biology. While many biological factors associated with evolu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt349
更新日期:2013-07-01 00:00:00
abstract::A system for the tetracycline-inducible regulation of gene expression in mycobacteria has been developed. We have sub-cloned the tetRO region from the Corynebacterium glutamicum TetZ locus into a mycobacterial shuttle plasmid, making expression of genes cloned downstream of tetRO responsive to tetracycline. Using the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni023
更新日期:2005-02-01 00:00:00
abstract::The Epstein-Barr virus gene BRLF1 encodes the transcription factor R, which is a sequence-specific DNA-binding protein important for the switch from latency to a productive cycle. We have defined a repertoire of specific R-binding sites using a GST-R fusion protein and a pool of 23 bp random DNA sequences. The R-bound...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.7.1172
更新日期:1994-04-11 00:00:00
abstract::A sensitive gel retention assay has been utilized to detect proteins from uninfected Hela nuclei which interact with the adenovirus type 2 enhancer. This assay has been employed to monitor fractionation of nuclear extracts. Three enhancer binding factors were resolved by chromatography on DEAE-Sepharose and one of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.6.2719
更新日期:1987-03-25 00:00:00
abstract::Specific binding of HIV-1 viral protein NCp7 to a unique 35-base RNA stem-loop SL1 is critical for formation and packaging of the genomic RNA dimer found within HIV-1 virions. NCp7 binding stimulates refolding of SL1 from a metastable kissing dimer (KD) into thermodynamically stable linear dimer (LD). Using UV melting...
journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::A 9.2 kb segment of the maxi-circle of Trypanosoma brucei mitochondrial DNA contains the genes for cytochrome c oxidase subunits I and II (coxI and coxII) and seven Unassigned Reading Frames ("URFs"). The genes for coxI and coxII display considerable homology at the aminoacid level (38 and 25%, respectively) to the co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.19.7327
更新日期:1984-10-11 00:00:00
abstract::Cytosines in genomic DNA are sometimes methylated. This affects many biological processes and diseases. The standard way of measuring methylation is to use bisulfite, which converts unmethylated cytosines to thymines, then sequence the DNA and compare it to a reference genome sequence. We describe a method for the cri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks275
更新日期:2012-07-01 00:00:00
abstract::We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutationa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.12.2318
更新日期:1994-06-25 00:00:00
abstract::The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast ce...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.8.1979
更新日期:1992-04-25 00:00:00
abstract::Mouse DNA contains two equally abundant, homologous subfamilies of MspI 3.6 and 5 kb repeated fragments. The first subfamily corresponds to the previously described (1) Bam 4 kb repeats, the second one to Bam repeated fragments of higher molecular weight. These subfamilies account for the vast majority of long Bam rep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.3.1593
更新日期:1984-02-10 00:00:00
abstract::Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1225
更新日期:2020-02-28 00:00:00
abstract::In kinetoplastid protozoa, mitochondrial (mt) mRNAs are post-transcriptionally edited by insertion and deletion of uridylate residues, the information being provided by guide (g)RNAs. Currently popular mechanisms for the editing process envisage a series of consecutive 'cut-and-paste' reactions, carried out by a compl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.14.2950
更新日期:2001-07-15 00:00:00
abstract::Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression tha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz181
更新日期:2019-05-21 00:00:00
abstract::Two new crescent-shaped unsymmetrical cyanine dyes have been synthesised and their interactions with DNA have been investigated by different spectroscopic methods. These dyes are analogues to a minor groove binding unsymmetrical cyanine dye, BEBO, recently reported by us. In this dye, the structure of the known interc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg821
更新日期:2003-11-01 00:00:00