A new minor groove binding asymmetric cyanine reporter dye for real-time PCR.

Abstract:

:The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing emission to be measured at the end of the elongation phase in the PCR cycle. BEBO concentrations between 0.1 and 0.4 micro M generated sufficient fluorescence signal without inhibiting the PCR. A comparison with the commonly used reporter dye SYBR Green I shows that the two dyes behave similarly in all important aspects.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Bengtsson M,Karlsson HJ,Westman G,Kubista M

doi

10.1093/nar/gng045

keywords:

subject

Has Abstract

pub_date

2003-04-15 00:00:00

pages

e45

issue

8

eissn

0305-1048

issn

1362-4962

journal_volume

31

pub_type

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