Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.

abstract：:Fast viral adaptation and the implication of this rapid evolution in the emergence of several new infectious diseases have turned this issue into a major challenge for various research domains. Indeed, viruses are involved in the development of a wide range of pathologies and understanding how viruses and host cells i...

journal_title：Nucleic acids research

authors： Palmeira L,Penel S,Lotteau V,Rabourdin-Combe C,Gautier C

更新日期：2011-01-01 00:00:00

abstract：:Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's...

journal_title：Nucleic acids research

authors： Kuzmich S,Marky LA,Jones RA

更新日期：1983-05-25 00:00:00

abstract：:The Orthologous Matrix (OMA) is a leading resource to relate genes across many species from all of life. In this update paper, we review the recent algorithmic improvements in the OMA pipeline, describe increases in species coverage (particularly in plants and early-branching eukaryotes) and introduce several new feat...

journal_title：Nucleic acids research

authors： Altenhoff AM,Glover NM,Train CM,Kaleb K,Warwick Vesztrocy A,Dylus D,de Farias TM,Zile K,Stevenson C,Long J,Redestig H,Gonnet GH,Dessimoz C

更新日期：2018-01-04 00:00:00

abstract：:The (cytosine-5) DNA methyltransferase M.HhaI causes its target cytosine base to be flipped completely out of the DNA helix upon binding. We have investigated the effects of replacing the target cytosine by other, mismatched bases, including adenine, guanine, thymine and uracil. We find that M.HhaI binds more tightly ...

journal_title：Nucleic acids research

authors： Klimasauskas S,Roberts RJ

更新日期：1995-04-25 00:00:00

abstract：:MicroRNAs (miRNAs) are short, non-protein coding RNAs that direct the widespread phenomenon of post-transcriptional regulation of metazoan genes. The mature approximately 22-nt long RNA molecules are processed from genome-encoded stem-loop structured precursor genes. Hundreds of such genes have been experimentally val...

journal_title：Nucleic acids research

authors： Gerlach D,Kriventseva EV,Rahman N,Vejnar CE,Zdobnov EM

更新日期：2009-01-01 00:00:00

abstract：:Positive control of the wild-type Klebsiella pneumoniae nifH promoter by the NifA protein requires that NifA is bound at the upstream activator sequence (UAS). By introducing base substitutions at -15 to -17 in the RNA polymerase recognition sequence of the nifH promoter, positive control by a form of NifA unable to b...

journal_title：Nucleic acids research

authors： Buck M,Cannon W

更新日期：1989-04-11 00:00:00

abstract：:Module identification is a frequently used approach for mining local structures with more significance in global networks. Recently, a wide variety of bilayer networks are emerging to characterize the more complex biological processes. In the light of special topological properties of bilayer networks and the accompan...

journal_title：Nucleic acids research

authors： Xu Y,Guo M,Liu X,Wang C,Liu Y,Liu G

更新日期：2016-11-16 00:00:00

abstract：:Some human cancers maintain their telomeres using the alternative lengthening of telomeres (ALT) mechanism; a process thought to involve recombination. Different types of recombinational telomere elongation pathways have been identified in yeasts. In senescing yeast telomerase deletion (ter1-Delta) mutants with very s...

journal_title：Nucleic acids research

authors： Basenko EY,Cesare AJ,Iyer S,Griffith JD,McEachern MJ

更新日期：2010-01-01 00:00:00

abstract：:The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly...

journal_title：Nucleic acids research

authors： Van Kreijl CF,Beelen RH,Borst P

更新日期：1977-02-01 00:00:00

abstract：:Telomeres comprise the protective caps of natural chromosome ends and function in the suppression of DNA damage signaling and cellular senescence. Therefore, techniques used to determine telomere length are important in a number of studies, ranging from those investigating telomeric structure to effects on human disea...

journal_title：Nucleic acids research

authors： Göhring J,Fulcher N,Jacak J,Riha K

更新日期：2014-02-01 00:00:00

abstract：:The RSC complex of Saccharomyces cerevisiae is closely related to the SWI/SNF complex. Both complexes are involved in remodeling chromatin structure and they share conserved components. The RSC proteins Sth1, Rsc8/Swh3, Sfh1 and Rsc6 are homologs of the SWI/SNF proteins Swi2/Snf2, Swi3, Snf5 and Swp73 respectively. To...

journal_title：Nucleic acids research

authors： Treich I,Ho L,Carlson M

更新日期：1998-08-15 00:00:00

abstract：:Human papillomavirus 16 (HPV-16), which is involved in genital carcinogenesis, contains an enhancer of transcription that is activated by cellular factors rather than by the viral E2 proteins. The activity resides on a 232 bp segment with 5 binding sites for nuclear factor 1 (NF1), 2 for AP1, and 1 for steroid recepto...

journal_title：Nucleic acids research

authors： Chong T,Chan WK,Bernard HU

更新日期：1990-02-11 00:00:00

abstract：:In vitro splicing of human beta-globin pre-mRNA can be fully inhibited by treatment of the splicing extract with polyclonal antibodies against hnRNP core proteins prior to the addition of pre-mRNA. Inhibition of the first step in the splicing pathway, cleavage at the 5' splice site and lariat formation, requires more ...

journal_title：Nucleic acids research

authors： Sierakowska H,Szer W,Furdon PJ,Kole R

更新日期：1986-07-11 00:00:00

abstract：:We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of micro...

journal_title：Nucleic acids research

authors： Wiegant J,Ried T,Nederlof PM,van der Ploeg M,Tanke HJ,Raap AK

更新日期：1991-06-25 00:00:00

abstract：:A 5',7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and a textbook aspect of co-transcriptional RNA processing. The cap is bound by the cap-binding complex (CBC), canonically consisting of nuclear cap-binding proteins 1 and 2 (NCBP1/2). Interest in the CBC has recently renewed...

journal_title：Nucleic acids research

authors： Dou Y,Kalmykova S,Pashkova M,Oghbaie M,Jiang H,Molloy KR,Chait BT,Rout MP,Fenyö D,Jensen TH,Altukhov I,LaCava J

更新日期：2020-10-09 00:00:00

abstract：:Replication protein A (RPA) plays a critical role in all eukaryotic DNA processing involving single-stranded DNA (ssDNA). Contrary to the notion that RPA provides solely inert protection to transiently formed ssDNA, the RPA-ssDNA complex acts as a dynamic DNA processing unit. Here, we studied the diffusion of RPA alon...

journal_title：Nucleic acids research

authors： Mishra G,Bigman LS,Levy Y

更新日期：2020-02-28 00:00:00

abstract：:Cyclophilins are a family of proteins that share a common, highly conserved sequence motif. Cyclophilins bind transiently to other proteins and facilitate their folding. One member of the family, hCypH, is part of the human spliceosomal [U4/U6.U5] tri-snRNP complex; it associates specifically and stably with the U4/U6...

journal_title：Nucleic acids research

authors： Ingelfinger D,Göthel SF,Marahiel MA,Reidt U,Ficner R,Lührmann R,Achsel T

更新日期：2003-08-15 00:00:00

abstract：:Nitrated polycyclic aromatic hydrocarbons are common environmental pollutants, of which many are mutagenic and carcinogenic. 1-Nitropyrene is the most abundant nitrated polycyclic aromatic hydrocarbon, which causes DNA damage and is carcinogenic in experimental animals. Error-prone translesion synthesis of 1-nitropyre...

journal_title：Nucleic acids research

authors： Kirouac KN,Basu AK,Ling H

更新日期：2013-02-01 00:00:00

abstract：:PIWI-interacting RNAs (piRNAs) comprise a class of small RNAs best known for suppressing transposable elements in germline tissues. The vector mosquito Aedes aegypti encodes seven PIWI genes, four of which are somatically expressed. This somatic piRNA pathway generates piRNAs from viral RNA during infection with cytop...

journal_title：Nucleic acids research

authors： Joosten J,Miesen P,Taşköprü E,Pennings B,Jansen PWTC,Huynen MA,Vermeulen M,Van Rij RP

更新日期：2019-03-18 00:00:00

abstract：:Mammalian antibody switch regions (∼1500 bp) are composed of a series of closely neighboring G4-capable sequences. Whereas numerous structural and genome-wide analyses of roles for minimal G4s in transcriptional regulation have been reported, Long G4-capable regions (LG4s)-like those at antibody switch regions-remain ...

journal_title：Nucleic acids research

authors： Williams JD,Houserova D,Johnson BR,Dyniewski B,Berroyer A,French H,Barchie AA,Bilbrey DD,Demeis JD,Ghee KR,Hughes AG,Kreitz NW,McInnis CH,Pudner SC,Reeves MN,Stahly AN,Turcu A,Watters BC,Daly GT,Langley RJ,Gillesp

更新日期：2020-06-19 00:00:00

abstract：:Scalable and cost-effective production of error-free DNA is critical to meet the increased demand for such DNA in the field of biological science. Methods based on 'Dial-out PCR' have enabled the high-throughput error-free DNA synthesis from a microarray-synthesized DNA pool by labeling with retrieval PCR tags, and re...

journal_title：Nucleic acids research

authors： Lim H,Cho N,Ahn J,Park S,Jang H,Kim H,Han H,Lee JH,Bang D

更新日期：2018-04-20 00:00:00

abstract：:We have previously reported that transcription from a distal promoter (promoter B) of the estrogen receptor alpha (ERalpha) gene is responsible for the increased expression of ERalpha in human breast carcinomas. This paper first characterized the promoter B region in terms of transient transfection experiments with lu...

journal_title：Nucleic acids research

authors： Tanimoto K,Eguchi H,Yoshida T,Hajiro-Nakanishi K,Hayashi S

更新日期：1999-02-01 00:00:00

abstract：:Human Adenovirus type 5 encodes two short RNA polymerase III transcripts, the virus-associated (VA) RNAI and VA RNAII, which can adopt stable hairpin structures that resemble micro-RNA precursors. The terminal stems of the VA RNAs are processed into small RNAs (mivaRNAs) that are incorporated into RISC. It has been re...

journal_title：Nucleic acids research

authors： Xu N,Gkountela S,Saeed K,Akusjärvi G

更新日期：2009-11-01 00:00:00

abstract：:The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenoty...

journal_title：Nucleic acids research

authors： Triman KL

更新日期：1996-01-01 00:00:00

abstract：:Ribosome biogenesis (RiBi) is an extremely energy intensive process that is critical for gene expression. It is thus highly regulated, including through the tightly coordinated expression of over 200 RiBi genes by positive and negative transcriptional regulators. We investigated RiBi regulation as cells initiated meio...

journal_title：Nucleic acids research

authors： Cheng Z,Brar GA

更新日期：2019-06-04 00:00:00

abstract：:EcoRI digestion of total mouse DNA yields a prominant 1.3 kb fragment amounting to between 1 and 2% of the mouse genome. The majority of the 1.3 kb EcoRI fragments have a single Bg1II site 800 bp from one end. This EcoRI-Bg1II sequence family shows HindIII and HaeIII sequence heterogeneity. We have cloned representati...

journal_title：Nucleic acids research

authors： Heller R,Arnheim N

更新日期：1980-11-11 00:00:00

abstract：:Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzym...

journal_title：Nucleic acids research

authors： Lee YH,Blakesley RW,Smith LA,Chirikjian JG

更新日期：1978-03-01 00:00:00

abstract：:A comparison of gene structure, sequence, and transcription pattern of heat shock loci 93D of Drosophila melanogaster and 48B of Drosophila hydei has been performed. Both heat shock loci consist of an unique region that is flanked by an internally repetitive element. Different members of these elements are highly cons...

journal_title：Nucleic acids research

authors： Ryseck RP,Walldorf U,Hoffmann T,Hovemann B

更新日期：1987-04-24 00:00:00

abstract：:The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic euryarchaeon Thermococcus aggregans. This motif plays a critical role in the balance between the synthesis and degradation of the DNA chain. Five different mutations of th...

journal_title：Nucleic acids research

authors： Böhlke K,Pisani FM,Vorgias CE,Frey B,Sobek H,Rossi M,Antranikian G

更新日期：2000-10-15 00:00:00

abstract：:The deoxyoligonucleotide 5' AATCGGGCATGGATT (15-mer) was synthesized containing 12 phosphoramidate linkages derived from 2 primary and 2 secondary amines. The oligonucleotides were purified by reverse phase HPLC and characterized by PAGE. The thermal stability of the duplexes derived from these compounds, when hybridi...

journal_title：Nucleic acids research

authors： Froehler B,Ng P,Matteucci M

更新日期：1988-06-10 00:00:00