Abstract:
:The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Van Kreijl CF,Beelen RH,Borst Pdoi
10.1093/nar/4.2.445subject
Has Abstractpub_date
1977-02-01 00:00:00pages
445-55issue
2eissn
0305-1048issn
1362-4962journal_volume
4pub_type
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