On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis.

Abstract:

:The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Van Kreijl CF,Beelen RH,Borst P

doi

10.1093/nar/4.2.445

subject

Has Abstract

pub_date

1977-02-01 00:00:00

pages

445-55

issue

2

eissn

0305-1048

issn

1362-4962

journal_volume

4

pub_type

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