On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis.


:The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.


Nucleic Acids Res


Nucleic acids research


Van Kreijl CF,Beelen RH,Borst P




Has Abstract


1977-02-01 00:00:00












  • Amplification of genomic sequences flanking transposable elements in host and heterologous plants: a tool for transposon tagging and genome characterization.

    abstract::The isolation of sequences flanking integrated transposable elements is an important step in gene tagging strategies. We have demonstrated that sequences flanking transposons integrated into complex genomes can be simply and rapidly obtained using the polymerase chain reaction. Amplification of such sequences was esta...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Earp DJ,Lowe B,Baker B

    更新日期:1990-06-11 00:00:00

  • BSD: the Biodegradative Strain Database.

    abstract::The Biodegradative Strain Database (BSD) is a freely-accessible, web-based database providing detailed information on degradative bacteria and the hazardous substances that they degrade, including corresponding literature citations, relevant patents and links to additional web-based biological and chemical data. The B...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Urbance JW,Cole J,Saxman P,Tiedje JM

    更新日期:2003-01-01 00:00:00

  • The Mouse Genome Database (MGD): genetic and genomic information about the laboratory mouse. The Mouse Genome Database Group.

    abstract::The Mouse Genome Database (MGD) focuses on the integration of mapping, homology, polymorphism and molecular data about the laboratory mouse. Detailed descriptions of genes including their chromosomal location, gene function, disease associations, mutant phenotypes, molecular polymorphisms and links to representative s...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Blake JA,Richardson JE,Davisson MT,Eppig JT

    更新日期:1999-01-01 00:00:00

  • GenBank.

    abstract::The GenBank((R))sequence database incorporates publicly available DNA sequences of >55 000 different organisms, primarily through direct submission of sequence data from individual laboratories and large-scale sequencing projects. Most submissions are made using the BankIt (Web) or Sequin programs and accession number...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Benson DA,Karsch-Mizrachi I,Lipman DJ,Ostell J,Rapp BA,Wheeler DL

    更新日期:2000-01-01 00:00:00

  • Investigating the sequence-dependent mechanical properties of DNA nicks for applications in twisted DNA nanostructure design.

    abstract::DNA nick can be used as a design motif in programming the shape and reconfigurable deformation of synthetic DNA nanostructures, but its mechanical properties have rarely been systematically characterized at the level of base sequences. Here, we investigated sequence-dependent mechanical properties of DNA nicks through...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lee JY,Kim YJ,Lee C,Lee JG,Yagyu H,Tabata O,Kim DN

    更新日期:2019-01-10 00:00:00

  • The leader region of Laminin B1 mRNA confers cap-independent translation.

    abstract::Translation initiation of eukaryotic mRNAs generally occurs by cap-dependent ribosome scanning. However, certain mRNAs contain internal ribosome entry sites (IRES) allowing cap-independent translation. Several of these IRES-competent transcripts and their corresponding proteins are involved in tumourigenesis. This stu...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Petz M,Kozina D,Huber H,Siwiec T,Seipelt J,Sommergruber W,Mikulits W

    更新日期:2007-01-01 00:00:00

  • Dynamic topology of double-stranded telomeric DNA studied by single-molecule manipulation in vitro.

    abstract::The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric DNA. Herein, we developed an EM-PCR and TA cloning-based approach to s...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zhang X,Zhang Y,Zhang W

    更新日期:2020-07-09 00:00:00

  • Intrinsic differences between authentic and cryptic 5' splice sites.

    abstract::Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5' splice sites (5'ss), we systematically analyzed a set of 76 cryptic 5'ss derived from 46 human genes. These cryptic 5'ss have a similar frequency distribut...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Roca X,Sachidanandam R,Krainer AR

    更新日期:2003-11-01 00:00:00

  • Human Disease Ontology 2018 update: classification, content and workflow expansion.

    abstract::The Human Disease Ontology (DO) (http://www.disease-ontology.org), database has undergone significant expansion in the past three years. The DO disease classification includes specific formal semantic rules to express meaningful disease models and has expanded from a single asserted classification to include multiple-...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schriml LM,Mitraka E,Munro J,Tauber B,Schor M,Nickle L,Felix V,Jeng L,Bearer C,Lichenstein R,Bisordi K,Campion N,Hyman B,Kurland D,Oates CP,Kibbey S,Sreekumar P,Le C,Giglio M,Greene C

    更新日期:2019-01-08 00:00:00

  • Sequence determination of nucleic acids containing 5-methylisocytosine and isoguanine: identification and insight into polymerase replication of the non-natural nucleobases.

    abstract::Nucleobase analogs 5-methylisocytosine ((Me)isoC) and isoguanine (isoG) form a non-natural base pair in duplex nucleic acids with base pairing specificity orthogonal to the natural nucleobase pairs. Sequencing reactions were conducted with oligodeoxyribonucleotides (ODNs) containing d(Me)isoC and disoG using modified ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ahle JD,Barr S,Chin AM,Battersby TR

    更新日期:2005-06-02 00:00:00

  • Genomicus 2018: karyotype evolutionary trees and on-the-fly synteny computing.

    abstract::Since 2010, the Genomicus web server is available online at http://genomicus.biologie.ens.fr/genomicus. This graphical browser provides access to comparative genomic analyses in four different phyla (Vertebrate, Plants, Fungi, and non vertebrate Metazoans). Users can analyse genomic information from extant species, as...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Nguyen NTT,Vincens P,Roest Crollius H,Louis A

    更新日期:2018-01-04 00:00:00

  • The H19/let-7 double-negative feedback loop contributes to glucose metabolism in muscle cells.

    abstract::The H19 lncRNA has been implicated in development and growth control and is associated with human genetic disorders and cancer. Acting as a molecular sponge, H19 inhibits microRNA (miRNA) let-7. Here we report that H19 is significantly decreased in muscle of human subjects with type-2 diabetes and insulin resistant ro...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gao Y,Wu F,Zhou J,Yan L,Jurczak MJ,Lee HY,Yang L,Mueller M,Zhou XB,Dandolo L,Szendroedi J,Roden M,Flannery C,Taylor H,Carmichael GG,Shulman GI,Huang Y

    更新日期:2014-12-16 00:00:00

  • PharmaGist: a webserver for ligand-based pharmacophore detection.

    abstract::Predicting molecular interactions is a major goal in rational drug design. Pharmacophore, which is the spatial arrangement of features that is essential for a molecule to interact with a specific target receptor, is an important model for achieving this goal. We present a freely available web server, named PharmaGist,...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schneidman-Duhovny D,Dror O,Inbar Y,Nussinov R,Wolfson HJ

    更新日期:2008-07-01 00:00:00

  • Effects of hypoxanthine substitution on bleomycin-mediated DNA strand degradation in DNA-RNA hybrids.

    abstract::We have reported on the differences in site-specific cleavage between DNA and DNA-RNA hybrids by various prototypic DNA cleavers (accompanying paper). In the case of bleomycin (BLM), degradation at 5'-GC-3'sites was suppressed relative to the same sequence in double-stranded DNA, while 5'-GT-3' damage remained constan...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bansal M,Stubbe J,Kozarich JW

    更新日期:1997-05-01 00:00:00

  • Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.

    abstract::Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from th...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Huang YQ,Rehfuss RP,LaPlante SR,Boudreau E,Borer PN,Lane MJ

    更新日期:1988-12-09 00:00:00

  • A transgenic mouse strain expressing four drug-selectable marker genes.

    abstract::Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor f...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tucker KL,Wang Y,Dausman J,Jaenisch R

    更新日期:1997-09-15 00:00:00

  • Oncogene activation of HIV-LTR-driven expression via the NF-kappa B binding sites.

    abstract::The Raf-1 proto-oncogene product is a highly regulated serine/threonine kinase that functions in signal transduction downstream from growth factor receptors and upstream from nuclear proto-oncogene products. Using a transient cotransfection assay we have found that activated Raf-1 activates expression from the HIV-LTR...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bruder JT,Heidecker G,Tan TH,Weske JC,Derse D,Rapp UR

    更新日期:1993-11-11 00:00:00

  • The NCBI Taxonomy database.

    abstract::The NCBI Taxonomy database (http://www.ncbi.nlm.nih.gov/taxonomy) is the standard nomenclature and classification repository for the International Nucleotide Sequence Database Collaboration (INSDC), comprising the GenBank, ENA (EMBL) and DDBJ databases. It includes organism names and taxonomic lineages for each of the...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Federhen S

    更新日期:2012-01-01 00:00:00

  • Master regulatory GATA transcription factors: mechanistic principles and emerging links to hematologic malignancies.

    abstract::Numerous examples exist of how disrupting the actions of physiological regulators of blood cell development yields hematologic malignancies. The master regulator of hematopoietic stem/progenitor cells GATA-2 was cloned almost 20 years ago, and elegant genetic analyses demonstrated its essential function to promote hem...

    journal_title:Nucleic acids research

    pub_type: 杂志文章,评审


    authors: Bresnick EH,Katsumura KR,Lee HY,Johnson KD,Perkins AS

    更新日期:2012-07-01 00:00:00

  • Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs.

    abstract::siRNAs confer sequence specific and robust silencing of mRNA. By virtue of these properties, siRNAs have become therapeutic candidates for disease intervention. However, their use as therapeutic agents can be hampered by unintended off-target effects by either or both strands of the siRNA duplex. We report here that u...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Vaish N,Chen F,Seth S,Fosnaugh K,Liu Y,Adami R,Brown T,Chen Y,Harvie P,Johns R,Severson G,Granger B,Charmley P,Houston M,Templin MV,Polisky B

    更新日期:2011-03-01 00:00:00

  • Characterization of genome-wide p53-binding sites upon stress response.

    abstract::The tumor suppressor p53 is a sequence-specific transcription factor, which regulates the expression of target genes involved in different stress responses. To understand p53's essential transcriptional functions, unbiased analysis of its DNA-binding repertoire is pivotal. In a genome-wide tiling ChIP-on-chip approach...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Smeenk L,van Heeringen SJ,Koeppel M,van Driel MA,Bartels SJ,Akkers RC,Denissov S,Stunnenberg HG,Lohrum M

    更新日期:2008-06-01 00:00:00

  • PREDBALB/c: a system for the prediction of peptide binding to H2d molecules, a haplotype of the BALB/c mouse.

    abstract::PRED(BALB/c) is a computational system that predicts peptides binding to the major histocompatibility complex-2 (H2(d)) of the BALB/c mouse, an important laboratory model organism. The predictions include the complete set of H2(d) class I (H2-K(d), H2-L(d) and H2-D(d)) and class II (I-E(d) and I-A(d)) molecules. The p...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zhang GL,Srinivasan KN,Veeramani A,August JT,Brusic V

    更新日期:2005-07-01 00:00:00

  • Cloning of Drosophila GCN5: conserved features among metazoan GCN5 family members.

    abstract::PCAF and hGCN5 are distinct human genes that encode proteins related to the yeast histone acetyltransferase and transcriptional adapter GCN5. The PCAF protein shares extensive similarity with the 439 amino acids of yGCN5, but it has an approximately 350 amino acid N-terminal extension that interacts with the transcrip...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Smith ER,Belote JM,Schiltz RL,Yang XJ,Moore PA,Berger SL,Nakatani Y,Allis CD

    更新日期:1998-06-15 00:00:00

  • Structural basis of the XPB-Bax1 complex as a dynamic helicase-nuclease machinery for DNA repair.

    abstract::Nucleotide excision repair (NER) is a major DNA repair pathway for a variety of DNA lesions. XPB plays a key role in DNA opening at damage sites and coordinating damage incision by nucleases. XPB is conserved from archaea to human. In archaea, XPB is associated with a nuclease Bax1. Here we report crystal structures o...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: DuPrez K,He F,Chen Z,Hilario E,Fan L

    更新日期:2020-06-19 00:00:00

  • A bichromatic fluorescent reporter for cell-based screens of alternative splicing.

    abstract::Alternative splicing is the primary source of proteome complexity in metazoans and its regulation shapes the proteome in response to shifting physiological requirements. We developed a bichromatic splicing reporter that uses a peculiar feature of some fluorescent protein coding regions to express two different fluores...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Orengo JP,Bundman D,Cooper TA

    更新日期:2006-01-01 00:00:00

  • Determination of affinity, stoichiometry and sequence selectivity of minor groove binder complexes with double-stranded oligodeoxynucleotides by electrospray ionization mass spectrometry.

    abstract::Electrospray mass spectrometry was evaluated regarding the reliability of the determination of the stoichiometries and equilibrium association constants from single spectra. Complexes between minor groove binders (Hoechst 33258, Hoechst 33342, DAPI, netropsin and berenil) and 12mer oligonucleotide duplexes with a cent...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Rosu F,Gabelica V,Houssier C,De Pauw E

    更新日期:2002-08-15 00:00:00

  • Nucleotide sequence of an external transcribed spacer in Xenopus laevis rDNA: sequences flanking the 5' and 3' ends of 18S rRNA are non-complementary.

    abstract::We have sequenced the external transcribed spacer (ETS) of a ribosomal transcription unit from Xenopus laevis, together with sections of the preceding non-transcribed spacer. Our analysis was carried out on the same cloned transcription unit as that from which the internal transcribed spacers (ITS) were previously seq...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Maden BE,Moss M,Salim M

    更新日期:1982-04-10 00:00:00

  • Transferring the purine 2-amino group from guanines to adenines in DNA changes the sequence-specific binding of antibiotics.

    abstract::The proposition that the 2-amino group of guanine plays a critical role in determining how antibiotics recognise their binding sites in DNA has been tested by relocating it, using tyrT DNA derivative molecules substituted with inosine plus 2,6-diaminopurine (DAP). Irrespective of their mode of interaction with DNA, su...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bailly C,Waring MJ

    更新日期:1995-03-25 00:00:00

  • Interactions of USF and Ku antigen with a human DNA region containing a replication origin.

    abstract::By means of a combination of ion-exchange and sequence-specific affinity chromatography techniques, we have purified to homogeneity two protein complexes binding in a human DNA region (B48) previously recognized to contain a DNA replication origin. The DNA sequence used for the protein purification (B48 binding site) ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tóth EC,Marusic L,Ochem A,Patthy A,Pongor S,Giacca M,Falaschi A

    更新日期:1993-07-11 00:00:00

  • A mathematical and computational framework for quantitative comparison and integration of large-scale gene expression data.

    abstract::Analysis of large-scale gene expression studies usually begins with gene clustering. A ubiquitous problem is that different algorithms applied to the same data inevitably give different results, and the differences are often substantial, involving a quarter or more of the genes analyzed. This raises a series of import...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hart CE,Sharenbroich L,Bornstein BJ,Trout D,King B,Mjolsness E,Wold BJ

    更新日期:2005-05-10 00:00:00