Abstract:
:In vitro splicing of human beta-globin pre-mRNA can be fully inhibited by treatment of the splicing extract with polyclonal antibodies against hnRNP core proteins prior to the addition of pre-mRNA. Inhibition of the first step in the splicing pathway, cleavage at the 5' splice site and lariat formation, requires more antibodies than inhibition of the second step, cleavage at the 3' splice site and exon ligation. The anti-hnRNP antibodies can also inhibit the splicing reaction after the formation of the active nucleoprotein splicing complex which is known to occur during the initial lag period. Thus, hnRNP core proteins appear to be present in the complex that performs pre-mRNA splicing.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sierakowska H,Szer W,Furdon PJ,Kole Rdoi
10.1093/nar/14.13.5241subject
Has Abstractpub_date
1986-07-11 00:00:00pages
5241-54issue
13eissn
0305-1048issn
1362-4962journal_volume
14pub_type
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