Abstract:
:Telomere G-quadruplex is emerging as a promising anti-cancer target due to its inhibition to telomerase, an enzyme expressed in more than 85% tumors. Telomerase-mediated telomere extension and some other reactions require a free 3' telomere end in single-stranded form. G-quadruplex formation near the 3' end of telomere DNA can leave a 3' single-stranded tail of various sizes. How these terminal structures affect reactions at telomere end is not clear. In this work, we studied the 3' tail size-dependence of telomere extension by either telomerase or the alternative lengthening of telomere (ALT) mechanism as well as telomere G-quadruplex unwinding. We show that these reactions require a minimal tail of 8, 12 and 6 nt, respectively. Since we have shown that G-quadruplex tends to form at the farthest 3' distal end of telomere DNA leaving a tail of no more than 5 nt, these results imply that G-quadruplex formation may play a role in regulating reactions at the telomere ends and, as a result, serve as effective drug target for intervening telomere function.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wang Q,Liu JQ,Chen Z,Zheng KW,Chen CY,Hao YH,Tan Zdoi
10.1093/nar/gkr164subject
Has Abstractpub_date
2011-08-01 00:00:00pages
6229-37issue
14eissn
0305-1048issn
1362-4962pii
gkr164journal_volume
39pub_type
杂志文章abstract::DNA nick can be used as a design motif in programming the shape and reconfigurable deformation of synthetic DNA nanostructures, but its mechanical properties have rarely been systematically characterized at the level of base sequences. Here, we investigated sequence-dependent mechanical properties of DNA nicks through...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1189
更新日期:2019-01-10 00:00:00
abstract::A coiled-coil microtubule-bundling protein, p180, was originally identified as one of the ribosome receptor candidates on the rough endoplasmic reticulum (ER) and is highly expressed in secretory tissues. Recently, we reported that p180 plays crucial roles in upregulating collagen biosynthesis, mainly by facilitating ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1197
更新日期:2012-04-01 00:00:00
abstract::RNA is emerging as a key regulator of a plethora of biological processes. While its study has remained elusive for decades, the recent advent of high-throughput sequencing technologies provided the unique opportunity to develop novel techniques for the study of RNA structure and post-transcriptional modifications. Non...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky486
更新日期:2018-09-19 00:00:00
abstract::GC-AG introns represent 0.7% of total human pre-mRNA introns. To study the function of GC-AG introns in splicing regulation, 196 cDNA-confirmed GC-AG introns were identified in Caenorhabditis elegans. These represent 0.6% of the cDNA- confirmed intron data set for this organism. Eleven of these GC-AG introns are invol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf465
更新日期:2002-08-01 00:00:00
abstract::PARP1 and PARP2 are implicated in the synthesis of poly(ADP-ribose) (PAR) after detection of DNA damage. The specificity of PARP1 and PARP2 interaction with long DNA fragments containing single- and/or double-strand breaks (SSBs and DSBs) have been studied using atomic force microscopy (AFM) imaging in combination wit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1476
更新日期:2016-04-07 00:00:00
abstract::The 34-nucleotide trans-activator (TA) located within the RNA-2 of Red clover necrotic mosaic virus folds into a simple hairpin. The eight-nucleotide TA loop base pairs with eight complementary nucleotides in the TA binding sequence (TABS) of the capsid protein subgenomic promoter on RNA-1 and trans-activates subgenom...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh585
更新日期:2004-05-20 00:00:00
abstract::The centromere complex is a multicomponent structure essential for faithful chromosome transmission. Here we show that the S. cerevisiae centromere protein Cpf1 bends centromere DNA element I (CDEI) with the bend angle ranging from 66 degrees to 71 degrees. CDEI DNA sequences that carry point mutations which lead to r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.20.4726
更新日期:1993-10-11 00:00:00
abstract::Isopentenyl adenosine derivatives are always located adjacent to the 3' end of the anticodon in transfer RNA and have been implicated in certain biological functions. In the enteric bacterium, E. coli, the derivative is ms2i6A whereas in some plant associated bacteria the derivative is the hydroxylated form, ms2io6A. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.18.5663
更新日期:1982-09-25 00:00:00
abstract::A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.19.4395
更新日期:1998-10-01 00:00:00
abstract::The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.8.2683
更新日期:1979-06-25 00:00:00
abstract::Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.6.823
更新日期:1974-06-01 00:00:00
abstract::Targeted modulation of gene expression represents a valuable approach to understand the mechanisms governing gene regulation. In a therapeutic context, it can be exploited to selectively modify the aberrant expression of a disease-causing gene or to provide the target cells with a new function. Here, we have establish...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky171
更新日期:2018-05-18 00:00:00
abstract::The smallest catalytic RNA identified to date is a manganese-dependent ribozyme that requires only a complex between GAAA and UUU to effect site-specific cleavage. We show here that this ribozyme occurs naturally in the 3'-UTR of Vg1 and beta-actin mRNAs. In accord with earlier studies with model RNAs, cleavage occurs...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn530
更新日期:2008-10-01 00:00:00
abstract::The interactions of HIV-1 reverse transcriptase (HIV-1 RT) with a synthetic 53/19-mer DNA substrate was investigated. For this template-primer HIV-1 RT displayed a Km value of 20 nM. The 53/19-mer competitively inhibited DNA synthesis performed on poly (rC).oligo(dG) with Ki value of 260 nM. This corresponded well to ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.17.3943
更新日期:1993-08-25 00:00:00
abstract::Thyroid hormone receptors (TRs) are members of the nuclear hormone receptor superfamily, which act as transcription factors upon binding to specific DNA sequences called thyroid hormone (T3) response elements (TREs). Such elements are found in the upstream regulatory region of promoters as well as in intragenic sequen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.9.1774
更新日期:1997-05-01 00:00:00
abstract::The titration viscometric investigation of the multi-mode interaction of netropsin (Nt) with (A.T) clusters of NaDNA12 and NH4DNA10 has been extended to different temperatures. The position of two boundaries on the r-scale (r= [Nt]bound/[DNA-P]) with increasing temperature steadily (rI/II) or more abruptly (rO/I) shif...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.10.2335
更新日期:1981-05-25 00:00:00
abstract::Phosphoprotein-binding domains (PPBDs) mediate many important cellular and molecular processes. Ten PPBDs have been known to exist in the human proteome, namely, 14-3-3, BRCT, C2, FHA, MH2, PBD, PTB, SH2, WD-40 and WW. PepCyber:P approximately PEP is a newly constructed database specialized in documenting human PPBD-c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm854
更新日期:2008-01-01 00:00:00
abstract::As a trial practical application, we have applied optimized microfabricated electrophoresis devices, combined with enzymatic mutation detection methods, to the determination of single nucleotide polymorphism (SNP) sites in the p53 suppressor gene. Using clinical samples, we have achieved robust assays with quality fac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.9.e43
更新日期:2000-05-01 00:00:00
abstract::Recent genome-wide analyses have elucidated the extent of alternative splicing (AS) in mammals, often focusing on comparisons of splice isoforms between differentiated tissues. However, regulated splicing changes are likely to be important in biological transitions such as cellular differentiation, or response to envi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq614
更新日期:2010-11-01 00:00:00
abstract::Six 1',5'-anhydrohexitol uridine triphosphates were synthesized with aromatic substitutions appended via a carboxamide linker to the 5-position of their bases. An improved method for obtaining such 5-substituted hexitol nucleosides and nucleotides is described. The incorporation profile of the nucleotide analogues int...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz008
更新日期:2019-03-18 00:00:00
abstract::The organelle genome database GOBASE, now in its 21st release (June 2008), contains all published mitochondrion-encoded sequences (approximately 913,000) and chloroplast-encoded sequences (approximately 250,000) from a wide range of eukaryotic taxa. For all sequences, information on related genes, exons, introns, gene...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn819
更新日期:2009-01-01 00:00:00
abstract::Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo cell-free protein synthesis system shows a heterogeneous but equal distribution of these abundant translatable mRNAs in...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.17.3841
更新日期:1980-09-11 00:00:00
abstract::We report a novel system for efficient and specific targeted delivery of large nucleic acids to and into cells. Plasmid DNA and core histones were assembled to chromatin by salt gradient dialysis and subsequently connected to bispecific antibody derivatives (bsAbs) via a nucleic acid binding peptide bridge. The result...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz137
更新日期:2019-06-04 00:00:00
abstract::We provide a novel web service, called rQuant.web, allowing convenient access to tools for quantitative analysis of RNA sequencing data. The underlying quantitation technique rQuant is based on quadratic programming and estimates different biases induced by library preparation, sequencing and read mapping. It can tack...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq448
更新日期:2010-07-01 00:00:00
abstract::By subtractive screening of a library made from mRNA of lipopolysaccharide (LPS)-stimulated mouse B lymphocytes we isolated cDNA-clones encoding the ribosomal protein L7. Human L7 mRNA was cloned from activated T-lymphocytes. Although no specific function of L7 in the translation apparatus is known as yet, it should b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.2.223
更新日期:1993-01-25 00:00:00
abstract::The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.11.1549
更新日期:1974-11-01 00:00:00
abstract::Aside from classical loops among G-quadruplexes, the unique leaped V-shape scaffold spans over three G-tetrads, without any intervening residues. This scaffold enables a sharp reversal of two adjacent strand directions and simultaneously participates in forming the G-tetrad core. These features make this scaffold itse...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1167
更新日期:2019-02-20 00:00:00
abstract::Oligodeoxyribonucleotides containing N4-methoxycytosine (mo4C), N4-methoxy-5-methylcytosine (mo4m5C) and other base-analogues were synthesised and used to compare the stabilities of duplexes containing mo4C.A and mo4C.G base pairs with those containing normal and mismatch pairs. The Tm values and other thermodynamic p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.20.8167
更新日期:1987-10-26 00:00:00
abstract::Previous studies have established a critical role of both TFIIB and RNA polymerase II (RNAPII) in start site selection in the yeast Saccharomyces cerevisiae. However, it remains unclear how the TFIIB-RNAPII interaction impacts on this process since such an interaction can potentially influence both preinitiation compl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf422
更新日期:2002-07-15 00:00:00
abstract::A repeated non-coding DNA sequence d(TTAGGG)n is present in the telomeric ends of all human chromosomes. These repeats can adopt multiple inter and intramolecular non-B-DNA conformations that may play an important role in biological processes. Two intramolecular structures of the telomeric oligonucleotide dAGGG(TTAGGG...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh875
更新日期:2004-10-08 00:00:00