Abstract:
:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding to 50 mug of enzyme protein. Under similar conditions, derivatives DMAO and AF/MO failed to inhibit enzyme activity. Studies with PR/19 showed that the drug interacted directly with the enzyme molecule and did not affect the enzyme-primer complex formation. The inhibition by the drug could be reversed by increasing the substrate (ATP) concentration. It is concluded that some rifamycin derivatives can specifically inhibit template-independent nucleotide chain elongation reactions.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Jacob ST,Rose KMdoi
10.1093/nar/1.11.1549keywords:
subject
Has Abstractpub_date
1974-11-01 00:00:00pages
1549-59issue
11eissn
0305-1048issn
1362-4962journal_volume
1pub_type
杂志文章abstract::The sequencing of the full transcriptome (RNA-seq) has become the preferred choice for the measurement of genome-wide gene expression. Despite its widespread use, challenges remain in RNA-seq data analysis. One often-overlooked aspect is normalization. Despite the fact that a variety of factors or 'batch effects' can ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv736
更新日期:2015-09-18 00:00:00
abstract::Several modified nucleosides were introduced during in vitro RNA synthesis into a pre-tRNA(Ser). The pre-tRNAs were used as substrates for RNase P enzymes. No effects were observed with biotin-8-ATP or [alpha-S]-GPT, whereas with m7GTP, the cleavage reaction was completely inhibited. Analysis of pre-tRNAs which contai...
journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::The 279-bp major breakpoint region (mbr) within the 3'-untranslated region (3'-UTR) of the BCL2 gene is a binding site of special AT-rich sequence binding protein 1 (SATB1) that is well known to participate in the long-range regulation of gene transcription. Our previous studies have revealed that the mbr could regula...
journal_title:Nucleic acids research
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更新日期:2011-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni005
更新日期:2005-01-07 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-09-26 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm615
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2006-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks887
更新日期:2012-11-01 00:00:00
abstract::Integration of foreign genes into plant genomes by the Agrobacterium T-DNA transfer system has been considered to occur at random. It has been speculated that the chromosomal structure of the integration site might affect the expression pattern of the introduced genes. To gain insight into the molecular structure of T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.14.2744
更新日期:1994-07-25 00:00:00
abstract::The nucleotide sequence of the chick beta-actin gene was determined. The gene contains 5 introns; 4 interrupt the translated region at codons 41/42, 120/122, 267, 327/328 and a large intron occurs in the 5' untranslated region. The gene has a 97 nucleotide 5'-untranslated region and a 594 nucleotide 3'-untranslated re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.23.8287
更新日期:1983-12-10 00:00:00
abstract::DNA labeled for 15 minutes during UV induced repair synthesis is two-fold more sensitive to micrococcal nuclease than the bulk nuclear DNA. As the length of the labeling period increases from 15 minutes to 4 hours the nuclease sensitivity of repair labeled DNA approaches that of bulk chromatin. Pulse-chase experiments...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.1.203
更新日期:1981-01-10 00:00:00
abstract::Hg-UMP-containing transcripts made from chick erythroid chromatins with E. coli RNA polymerase hybridize to chick globin cDNA. Contamination with endogenous globin RNA has been largely removed by purification on SH-agarose columns at 55 degrees C. Some endogenous globin mRNA sequences remain, probably as hybrids with ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.4.1237
更新日期:1978-04-01 00:00:00
abstract::The underlying mechanism of transcriptional co-repressor ETO2 during early erythropoiesis and hemoglobin switching is unclear. We find that absence of ETO2 in mice interferes with down-regulation of PU.1 and GATA2 in the fetal liver, impeding a key step required for commitment to erythroid maturation. In human β-globi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa736
更新日期:2020-10-09 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa743
更新日期:2020-10-09 00:00:00
abstract::Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.16.5015
更新日期:1982-08-25 00:00:00
abstract::Infection of cultured cells with herpes simplex virus (HSV) results in the transcriptional induction of a small number of cellular genes. Although the majority of such genes are dependent upon viral protein synthesis for their induction, a small minority are not. These genes are induced by events occurring prior to th...
journal_title:Nucleic acids research
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doi:10.1093/nar/14.23.9261
更新日期:1986-12-09 00:00:00
abstract::Radioactive iodine has been used to probe the relative reactivities of nucleosomal H4 tyrosine residues under various conditions of subphysiological ionic strength. We observe that tyrosine 72 of H4, which is not reactive over the range 20-150 mM NaCl, becomes the predominant site of iodination within H4 when nucleoso...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1981-09-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part2.521
更新日期:1984-01-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
abstract::The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1013
更新日期:2020-12-16 00:00:00
abstract::Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (sha...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
abstract::Random amplified polymorphic DNA (RAPD) markers are used widely to develop high resolution genetic maps and for genome fingerprinting. Typically, single oligomers of approximately 10 nucleotides are used to PCR amplify characteristic RAPD marker fragments. We describe an efficient method for the direct end-sequencing ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.19.e28
更新日期:1999-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt349
更新日期:2013-07-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2011-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.14.2648
更新日期:1996-07-15 00:00:00
abstract::A molecular rotor thioflavin T (ThT) is usually used as a fluorescent ligand specific for G-quadruplexes. Here, we demonstrate that ThT can tightly bind non-G-quadruplex DNAs with several GA motifs and dimerize them in a parallel double-stranded mode, accompanied by over 100-fold enhancement in the fluorescence emissi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx942
更新日期:2017-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gks281
更新日期:2012-07-01 00:00:00