Abstract:
:Random amplified polymorphic DNA (RAPD) markers are used widely to develop high resolution genetic maps and for genome fingerprinting. Typically, single oligomers of approximately 10 nucleotides are used to PCR amplify characteristic RAPD marker fragments. We describe an efficient method for the direct end-sequencing of gel-purified RAPD fragments using one primer from a set of four 3'-terminal extended (A, T, C or G) oligonucleotides, identical to the RAPD primer but for the single nucleotide extension. Strand-specific DNA sequence could be independently read from each of the RAPD fragments without recourse to strand separation or fragment cloning. Informative RAPD fragments could be readily converted into mapped STS or SCAR loci using this technology. The 3'-extended primers may also be used to amplify independent genomic RAPD markers.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Mitchelson KR,Drenth J,Duong H,Chaparro JXdoi
10.1093/nar/27.19.e28keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
e28issue
19eissn
0305-1048issn
1362-4962pii
gnc028journal_volume
27pub_type
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