Redox state of tumor suppressor p53 regulates its sequence-specific DNA binding in DNA-damaged cells by cysteine 277.


:Using a bio-oligo pull-down DNA-binding assay we investigated the binding capacity of endogenous, DNA damage-induced p53 in human diploid fibroblasts to several p53-responsive elements (REs) present in p53-regulated genes. During the course of p53 accumulation, we observed a decrease in p53 binding to the GADD45 but not to the p21(WAF1/CIP1) RE. Using mutated GADD45 sequences we show that this change is dependent on the presence of cytosines at position 3 in RE pentamers and on the p53 redox state. Site-directed mutagenesis experiments demonstrated that Cys277 (a residue directly contacting base 3 in a RE pentamer) is critical for differential regulation of GADD45 in DNA-damaged cells. These data represent a novel mechanism for differential affinity of p53 to distinct REs.


Nucleic Acids Res


Nucleic acids research


Buzek J,Latonen L,Kurki S,Peltonen K,Laiho M





Has Abstract


2002-06-01 00:00:00












  • Transition state stabilization by the 'high' motif of class I aminoacyl-tRNA synthetases: the case of Escherichia coli methionyl-tRNA synthetase.

    abstract::Methionyl-tRNA synthetase belongs to the class I aminoacyl-tRNA synthetase family characterized both by a catalytic center built around a Rossmann Fold and by the presence of the two peptidic marker sequences HIGH and KMSKS. In this study, the role of the 21HLGH24 motif of Escherichia coli methionyl-tRNA synthetase wa...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schmitt E,Panvert M,Blanquet S,Mechulam Y

    更新日期:1995-12-11 00:00:00

  • Structural properties and gene-silencing activity of chemically modified DNA-RNA hybrids with parallel orientation.

    abstract::We report, herein, a new class of RNAi trigger molecules based on the unconventional parallel hybridization of two oligonucleotide chains. We have prepared and studied several parallel stranded (ps) duplexes, in which the parallel orientation is achieved through incorporation of isoguanine and isocytosine to form reve...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Habibian M,Yahyaee-Anzahaee M,Lucic M,Moroz E,Martín-Pintado N,Di Giovanni LD,Leroux JC,Hall J,González C,Damha MJ

    更新日期:2018-02-28 00:00:00

  • The p16INK4a tumor suppressor controls p21WAF1 induction in response to ultraviolet light.

    abstract::p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, th...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Al-Mohanna MA,Al-Khalaf HH,Al-Yousef N,Aboussekhra A

    更新日期:2007-01-01 00:00:00

  • Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins.

    abstract::The PROSITE pattern Zinc_Finger_C2H2 was extended to permit the detection of all C2H2 zinc fingers and their parent proteins in the recently completed sequence of the yeast genome. Additionally, a new computer program was written that extracts other zinc binding motifs (non C2H2 'fingers'), overlapping with the classi...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Böhm S,Frishman D,Mewes HW

    更新日期:1997-06-15 00:00:00

  • T7 promoter contacts essential for promoter activity in vivo.

    abstract::T7 RNA polymerase promoters consist of a highly conserved 23 base-pair sequence that spans the site of the initiation of transcription (+1) and extends from -17 to +6. To determine the bases within the T7 consensus promoter that are essential for promoter function a library of mutant T7 promoters was constructed, and ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ikeda RA,Ligman CM,Warshamana S

    更新日期:1992-05-25 00:00:00

  • Proton magnetic resonance studies of ultraviolet-irradiated apurinic acid.

    abstract::In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Rahn RO,Schleich T

    更新日期:1974-08-01 00:00:00

  • CAF-1-induced oligomerization of histones H3/H4 and mutually exclusive interactions with Asf1 guide H3/H4 transitions among histone chaperones and DNA.

    abstract::Anti-silencing function 1 (Asf1) and Chromatin Assembly Factor 1 (CAF-1) chaperone histones H3/H4 during the assembly of nucleosomes on newly replicated DNA. To understand the mechanism of histone H3/H4 transfer among Asf1, CAF-1 and DNA from a thermodynamic perspective, we developed and employed biophysical approache...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Liu WH,Roemer SC,Port AM,Churchill ME

    更新日期:2012-12-01 00:00:00

  • Oxidation of a single active site suffices for the functional inactivation of the dimeric Bacillus subtilis OhrR repressor in vitro.

    abstract::Bacillus subtilis OhrR is a dimeric repressor that senses organic peroxides and regulates the expression of the OhrA peroxiredoxin. Derepression results from oxidation of an active site cysteine which ultimately results in formation of a mixed disulfide with a low molecular weight thiol, a cyclic sulfenamide, or overo...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Eiamphungporn W,Soonsanga S,Lee JW,Helmann JD

    更新日期:2009-03-01 00:00:00

  • Cloning of bovine growth hormone gene and its expression in bacteria.

    abstract::A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cl...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv H

    更新日期:1981-01-10 00:00:00

  • Nucleotide sequences from phaseolin cDNA clones: the major storage proteins from Phaseolus vulgaris are encoded by two unique gene families.

    abstract::The nucleotide sequences of eight partial and five full-length phaseolin cDNA clones show that phaseolin polypeptides are encoded by two distinct gene families which differ in their coding regions by the presence or absence of two different size direct repeats. The alpha-type phaseolin polypeptides are encoded by gene...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Slightom JL,Drong RF,Klassy RC,Hoffman LM

    更新日期:1985-09-25 00:00:00

  • A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand-nucleic acid structural selectivity.

    abstract::We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic acid binders containing a variety chemical scaffolds. We used a combination of principal c...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Del Villar-Guerra R,Gray RD,Trent JO,Chaires JB

    更新日期:2018-04-20 00:00:00

  • Changes in methylation pattern of albumin and alpha-fetoprotein genes in developing rat liver and neoplasia.

    abstract::To determine whether methylation changes in specific DNA sequences of the albumin and AFP genes are implicated in the modulation of transcriptional activity during rat liver development and neoplasia we have analysed the methylation pattern of C-C-G-G sequences within these genes in DNA isolated from fetal and adult h...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Vedel M,Gomez-Garcia M,Sala M,Sala-Trepat JM

    更新日期:1983-07-11 00:00:00

  • CHOP gene expression in response to endoplasmic-reticular stress requires NFY interaction with different domains of a conserved DNA-binding element.

    abstract::The transcription factor CHOP/GADD153 gene is induced by cellular stress and is involved in mediating apoptosis. We report the identification of a conserved region in the promoter of the CHOP gene responsible for its inducibility by endoplasmic reticulum (ER) stress. Deletion mutants of the human CHOP promoter identif...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ubeda M,Habener JF

    更新日期:2000-12-15 00:00:00

  • Replicative aging is associated with loss of genetic heterogeneity from extrachromosomal circular DNA in Saccharomyces cerevisiae.

    abstract::Circular DNA can arise from all parts of eukaryotic chromosomes. In yeast, circular ribosomal DNA (rDNA) accumulates dramatically as cells age, however little is known about the accumulation of other chromosome-derived circles or the contribution of such circles to genetic variation in aged cells. We profiled circular...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Prada-Luengo I,Møller HD,Henriksen RA,Gao Q,Larsen CE,Alizadeh S,Maretty L,Houseley J,Regenberg B

    更新日期:2020-08-20 00:00:00

  • Novel functional small RNAs are selectively loaded onto mammalian Ago1.

    abstract::Argonaute (Ago) proteins function in RNA silencing as components of the RNA-induced silencing complex (RISC). In lower organisms, the small interfering RNA and miRNA pathways diverge due in part to sorting mechanisms that direct distinct small RNA (sRNA) duplexes onto specific Ago-RISCs. However, such sorting mechanis...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Yamakawa N,Okuyama K,Ogata J,Kanai A,Helwak A,Takamatsu M,Imadome K,Takakura K,Chanda B,Kurosaki N,Yamamoto H,Ando K,Matsui H,Inaba T,Kotani A

    更新日期:2014-04-01 00:00:00

  • Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer.

    abstract::Overexpressed in colon carcinoma-1 (OCC-1) is one of the earliest annotated long noncoding RNAs (lncRNAs) in colorectal cancer (CRC); however, its function remains largely unknown. Here, we revealed that OCC-1 plays a tumor suppressive role in CRC. OCC-1 knockdown by RNA interference promotes cell growth both in vitro...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lan Y,Xiao X,He Z,Luo Y,Wu C,Li L,Song X

    更新日期:2018-06-20 00:00:00

  • Microamplification of specific chromosome sequences; an improved method for genome analysis.

    abstract::An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA templa...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ponelies N,Stein N,Weber G

    更新日期:1997-09-01 00:00:00

  • Dynamic changes in intron retention are tightly associated with regulation of splicing factors and proliferative activity during B-cell development.

    abstract::Intron retention (IR) has been proposed to modulate the delay between transcription and translation. Here, we provide an exhaustive characterization of IR in differentiated white blood cells from both the myeloid and lymphoid lineage where we observed highest levels of IR in monocytes and B-cells, in addition to previ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ullrich S,Guigó R

    更新日期:2020-02-20 00:00:00

  • PACRAT: a database and analysis system for archaeal and bacterial intergenic sequence features.

    abstract::Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ray WC,Daniels CJ

    更新日期:2003-01-01 00:00:00

  • Temperature mediated variation of DNA secondary structure in (A.T) clusters; evidence by use of the oligopeptide netropsin as a structural probe.

    abstract::The titration viscometric investigation of the multi-mode interaction of netropsin (Nt) with (A.T) clusters of NaDNA12 and NH4DNA10 has been extended to different temperatures. The position of two boundaries on the r-scale (r= [Nt]bound/[DNA-P]) with increasing temperature steadily (rI/II) or more abruptly (rO/I) shif...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Reinert KE,Geller D,Stutter E

    更新日期:1981-05-25 00:00:00

  • Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing.

    abstract::High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed. In this work, we have developed and implemented a set of synthetic 16S rRNA...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tourlousse DM,Yoshiike S,Ohashi A,Matsukura S,Noda N,Sekiguchi Y

    更新日期:2017-02-28 00:00:00

  • RNA conformational classes.

    abstract::RNA exhibits a large diversity of conformations. Three thousand nucleotides of 23S and 5S ribosomal RNA from a structure of the large ribosomal subunit were analyzed in order to classify their conformations. Fourier averaging of the six 3D distributions of torsion angles and analyses of the resulting pseudo electron m...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schneider B,Morávek Z,Berman HM

    更新日期:2004-03-11 00:00:00

  • Synthesis of 3'-phosphates of diribonucleoside monophosphates via phosphotriester intermediates.

    abstract::Phosphorylation of the easily accessible 3',5'-diesters 1a-d with diphenyl phosphorochloridate, followed by selective 5'-deacylation, affords the phosphotriester derivatives 2a-d in good yields. Alkaline treatment of 2a-d results in the formation of the 2',3'-cyclic phosphates (3a-d). The usefulness of the phosphotrie...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: van Boom JH,Burgers PM,Haasnoot CA

    更新日期:1976-10-01 00:00:00

  • RadA protein from Archaeoglobus fulgidus forms rings, nucleoprotein filaments and catalyses homologous recombination.

    abstract::Proteins that catalyse homologous recombination have been identified in all living organisms and are essential for the repair of damaged DNA as well as for the generation of genetic diversity. In bacteria homologous recombination is performed by the RecA protein, whereas in the eukarya a related protein called Rad51 i...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: McIlwraith MJ,Hall DR,Stasiak AZ,Stasiak A,Wigley DB,West SC

    更新日期:2001-11-15 00:00:00

  • 14-3-3tau associates with and activates the MEF2D transcription factor during muscle cell differentiation.

    abstract::Myocyte enhancer binding factor 2 (MEF2) proteins belong to the MADS box family of transcription factors and four MEF2 proteins, MEF2A, MEF2B, MEF2C and MEF2D, have been found. MEF2 proteins have been shown to play critical roles in differentiation of muscles and neuronal tissues. How transactivational activity of MEF...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Choi SJ,Park SY,Han TH

    更新日期:2001-07-01 00:00:00

  • Evidence for three distinct D proteins, which react differentially with anti-Sm autoantibodies, in the cores of the major snRNPs U1, U2, U4/U6 and U5.

    abstract::Electrophoresis of the mixture of proteins from purified snRNPs U1, U2, U4/U6 and U5 on SDS-polyacrylamide gels that had been allowed to polymerise in the presence of high TEMED concentrations have revealed the presence of proteins in the snRNPs that previously had eluded detection. The most striking case is that of p...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lehmeier T,Foulaki K,Lührmann R

    更新日期:1990-11-25 00:00:00

  • Compilation of DNA sequences of Escherichia coli (update 1993).

    abstract::We have compiled the DNA sequence data for E. coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the fifth listing replacing and increasing the former listings substantially. However, in order to save space this printed version contains...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kröger M,Wahl R,Rice P

    更新日期:1993-07-01 00:00:00

  • Stable propagation of cosmid sized human DNA inserts in an F factor based vector.

    abstract::Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have developed a low copy number cosmid vector based on the E. coli F factor replicon (Fosmid). We have tested relative stability o...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kim UJ,Shizuya H,de Jong PJ,Birren B,Simon MI

    更新日期:1992-03-11 00:00:00

  • A rat brain mRNA encoding a transcriptional activator homologous to the DNA binding domain of retroviral integrases.

    abstract::We have isolated a rat cDNA, named FE65, hybridizing to an mRNA of about 2,300 nucleotides present in rat brain, undetectable in rat liver and very poorly represented in other tissues. An mRNA of the same size is present in human neuroblastoma cells and is absent from other human cell lines. The FE65 cDNA contains an ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Duilio A,Zambrano N,Mogavero AR,Ammendola R,Cimino F,Russo T

    更新日期:1991-10-11 00:00:00

  • Effect of the BRCA2 CTRD domain on RAD51 filaments analyzed by an ensemble of single molecule techniques.

    abstract::Homologous recombination is essential for the preservation of genome stability, thereby preventing cancer. The recombination protein RAD51 drives DNA strand exchange, which requires the assembly, rearrangement and disassembly of a RAD51 filament on DNA, coupled to ATP binding and hydrolysis. This process is facilitate...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Holthausen JT,van Loenhout MT,Sanchez H,Ristic D,van Rossum-Fikkert SE,Modesti M,Dekker C,Kanaar R,Wyman C

    更新日期:2011-08-01 00:00:00