The Pol IV largest subunit CTD quantitatively affects siRNA levels guiding RNA-directed DNA methylation.

Abstract:

:In plants, nuclear multisubunit RNA polymerases IV and V are RNA Polymerase II-related enzymes that synthesize non-coding RNAs for RNA-directed DNA methylation (RdDM) and transcriptional gene silencing. Here, we tested the importance of the C-terminal domain (CTD) of Pol IV's largest subunit given that the Pol II CTD mediates multiple aspects of Pol II transcription. We show that the CTD is dispensable for Pol IV catalytic activity and Pol IV termination-dependent activation of RNA-DEPENDENT RNA POLYMERASE 2, which partners with Pol IV to generate dsRNA precursors of the 24 nt siRNAs that guide RdDM. However, 24 nt siRNA levels decrease ∼80% when the CTD is deleted. RNA-dependent cytosine methylation is also reduced, but only ∼20%, suggesting that siRNA levels typically exceed the levels needed for methylation of most loci. Pol IV-dependent loci affected by loss of the CTD are primarily located in chromosome arms, similar to loci dependent CLSY1/2 or SHH1, which are proteins implicated in Pol IV recruitment. However, deletion of the CTD does not phenocopy clsy or shh1 mutants, consistent with the CTD affecting post-recruitment aspects of Pol IV activity at target loci.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Wendte JM,Haag JR,Pontes OM,Singh J,Metcalf S,Pikaard CS

doi

10.1093/nar/gkz615

subject

Has Abstract

pub_date

2019-09-26 00:00:00

pages

9024-9036

issue

17

eissn

0305-1048

issn

1362-4962

pii

5536982

journal_volume

47

pub_type

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