PepCyber:P~PEP: a database of human protein protein interactions mediated by phosphoprotein-binding domains.

abstract：:Homopolyribonucleotides and E. coli DNA wer spin labeled with an iodoacetamide-nitroxide compound. The extent of labeling is highly dependent upon the nature of the base and the secondary structure of the nucleic acid. This spin label-polymer linkage is unstable at high temperatures and in phosphate buffers. In order ...

journal_title：Nucleic acids research

authors： Caspary WJ,Greene JJ,Stempel LM,Ts'o PO

更新日期：1976-04-01 00:00:00

abstract：:To organize data resulting from the phenotypic characterization of a library of 30,000 T-DNA enhancer trap (ET) insertion lines of rice (Oryza sativa L cv. Nipponbare), we developed the Oryza Tag Line (OTL) database (http://urgi.versailles.inra.fr/OryzaTagLine/). OTL structure facilitates forward genetic search for sp...

journal_title：Nucleic acids research

authors： Larmande P,Gay C,Lorieux M,Périn C,Bouniol M,Droc G,Sallaud C,Perez P,Barnola I,Biderre-Petit C,Martin J,Morel JB,Johnson AA,Bourgis F,Ghesquière A,Ruiz M,Courtois B,Guiderdoni E

更新日期：2008-01-01 00:00:00

abstract：:A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are generated between PCR products and a PCR-amplified plasmid vector. The procedure does not require the use of restriction enzymes, T4 DNA ligase or ...

journal_title：Nucleic acids research

authors： Aslanidis C,de Jong PJ

更新日期：1990-10-25 00:00:00

abstract：:This study describes a novel functional property of the eukaryotic transcription factor NF-kappa B in altering the DNA structure. We observed that the binding of purified NF-kappa B to DNA was facilitated by spermine and cations which are known to promote DNA bending, and by a nick in one position at the 3'-end of the...

journal_title：Nucleic acids research

authors： Schreck R,Zorbas H,Winnacker EL,Baeuerle PA

更新日期：1990-11-25 00:00:00

abstract：:The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residue...

journal_title：Nucleic acids research

authors： Yang CL,Chang LS,Zhang P,Hao H,Zhu L,Toomey NL,Lee MY

更新日期：1992-02-25 00:00:00

abstract：:We present the complete nucleotide sequence of the larval beta I-globin gene of Xenopus laevis including 240 nucleotides of the 5' flanking region and 594 nucleotides beyond the polyadenylation site. The site of transcription initiation was mapped by S1 nuclease, and the site of polyadenylation was determined by compa...

journal_title：Nucleic acids research

authors： Meyerhof W,Klinger-Mitropoulos S,Stalder J,Weber R,Knöchel W

更新日期：1984-10-25 00:00:00

abstract：:Traditional Chinese medicine (TCM) is not only an effective solution for primary health care, but also a great resource for drug innovation and discovery. To meet the increasing needs for TCM-related data resources, we developed ETCM, an Encyclopedia of Traditional Chinese Medicine. ETCM includes comprehensive and sta...

journal_title：Nucleic acids research

authors： Xu HY,Zhang YQ,Liu ZM,Chen T,Lv CY,Tang SH,Zhang XB,Zhang W,Li ZY,Zhou RR,Yang HJ,Wang XJ,Huang LQ

更新日期：2019-01-08 00:00:00

abstract：:A 34 base-pair (bp) fragment spanning sequences -154 to -120 of the promoter of the CHO1 gene (structural gene for phosphatidylserine synthase) from the yeast Saccharomyces cerevisiae has been shown to place transcription of a promoter-less Escherichia coli lacZ gene under control of the phospholipid precursors inosit...

journal_title：Nucleic acids research

authors： Bailis AM,Lopes JM,Kohlwein SD,Henry SA

更新日期：1992-03-25 00:00:00

abstract：:The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considerin...

journal_title：Nucleic acids research

authors： Tempestini A,Cassina V,Brogioli D,Ziano R,Erba S,Giovannoni R,Cerrito MG,Salerno D,Mantegazza F

更新日期：2013-02-01 00:00:00

abstract：:Yeast tRNA Val 1 alkylation with 2', 3'-O-4-(N-2-chloroethyl-N-methylamino) benzylidene d(pC-G)-A proceeds at 20 degrees - 30 degrees C in the complementary complexes which are formed by d(pC-G)-A greater than RC1 binding to 3 sequences of tRNA Val 1 : psi-C-G58 in the T loop, C-G40 at the 3'-side of the anticodon loo...

journal_title：Nucleic acids research

authors： Grineva NI,Karpova GG,Kuznetsova LM,Venkstern TV,Bayev AA

更新日期：1977-01-01 00:00:00

abstract：:Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5'-TTTN-3' protospacer adjacent motif (PAM) at the 5' end of the protospacer for genome editing. For widespread application in...

journal_title：Nucleic acids research

authors： Tu M,Lin L,Cheng Y,He X,Sun H,Xie H,Fu J,Liu C,Li J,Chen D,Xi H,Xue D,Liu Q,Zhao J,Gao C,Song Z,Qu J,Gu F

更新日期：2017-11-02 00:00:00

abstract：:Motor proteins that translocate on nucleic acids are key players in gene expression and maintenance. While the function of these proteins is diverse, they are driven by highly conserved core motor domains. In transcription-coupled DNA repair, motor activity serves to remove RNA polymerase stalled on damaged DNA, makin...

journal_title：Nucleic acids research

authors： Murphy MN,Gong P,Ralto K,Manelyte L,Savery NJ,Theis K

更新日期：2009-10-01 00:00:00

abstract：:Previous work in predicting protein localization to the chloroplast organelle in plants led to the development of an artificial neural network-based approach capable of remarkable accuracy in its prediction (ChloroP). A common criticism against such neural network models is that it is difficult to interpret the criter...

journal_title：Nucleic acids research

authors： Schein AI,Kissinger JC,Ungar LH

更新日期：2001-08-15 00:00:00

abstract：:The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between -318 and -300 and a proximal sequence element (PSE) centered at -55. Removal or alte...

journal_title：Nucleic acids research

authors： Wendelburg BJ,Marzluff WF

更新日期：1992-07-25 00:00:00

abstract：:In eukaryotes, DNA is packaged into a basic unit, the nucleosome which consists of 147 bp of DNA wrapped around a histone octamer composed of two copies each of the histones H2A, H2B, H3 and H4. Nucleosome structures are diverse not only by histone variants, histone modifications, histone composition but also through ...

journal_title：Nucleic acids research

authors： Ngo TT,Ha T

更新日期：2015-04-30 00:00:00

abstract：:Electrospray mass spectrometry was used to investigate the mechanism of tetramolecular G-quadruplex formation by the DNA oligonucleotide dTG(5)T, in ammonium acetate. The intermediates and products were separated according to their mass (number of strands and inner cations) and quantified. The study of the temporal ev...

journal_title：Nucleic acids research

authors： Rosu F,Gabelica V,Poncelet H,De Pauw E

更新日期：2010-08-01 00:00:00

abstract：:Elastic network models (ENMs) are valuable and efficient tools for characterizing the collective internal dynamics of proteins based on the knowledge of their native structures. The increasing evidence that the biological functionality of RNAs is often linked to their innate internal motions poses the question of whet...

journal_title：Nucleic acids research

authors： Pinamonti G,Bottaro S,Micheletti C,Bussi G

更新日期：2015-09-03 00:00:00

abstract：:Structural Maintenance of Chromosomes (SMC) proteins are vital for a wide range of processes including chromosome structure and dynamics, gene regulation and DNA repair. Eukaryotes have three SMC complexes, consisting of heterodimeric pairs of six different SMC proteins along with several specific regulatory subunits....

journal_title：Nucleic acids research

authors： Griese JJ,Witte G,Hopfner KP

更新日期：2010-06-01 00:00:00

abstract：:DNA sequences, that control expression of the spliced leader (SL) RNA gene in the parasitic protozoan Leishmania amazonensis, were mapped by block substitution mutagenesis. In the absence of a functional in vitro system for transcription, no promoter elements have yet been identified in this organism. We therefore dev...

journal_title：Nucleic acids research

authors： Agami R,Aly R,Halman S,Shapira M

更新日期：1994-06-11 00:00:00

abstract：:An alpha-neo-endorphin (alpha NE) gene, which we previously synthesized chemically and inserted into E. coli beta-galactosidase gene of pK013 plasmid, has been excised and fused to E. coli alkaline phosphatase (APase) gene. One of the transformants was named E15/pA alpha NE1. Under the APase gene regulation, APase-alp...

journal_title：Nucleic acids research

authors： Ohsuye K,Nomura M,Tanaka S,Kubota I,Nakazato H,Shinagawa H,Nakata A,Noguchi T

更新日期：1983-03-11 00:00:00

abstract：:Highly toxic DNA double-strand breaks (DSBs) readily trigger the DNA damage response (DDR) in cells, which delays cell cycle progression to ensure proper DSB repair. In Saccharomyces cerevisiae, mitotic S phase (20-30 min) is lengthened upon DNA damage. During meiosis, Spo11-induced DSB onset and repair lasts up to 5 ...

journal_title：Nucleic acids research

authors： Woo TT,Chuang CN,Higashide M,Shinohara A,Wang TF

更新日期：2020-09-04 00:00:00

abstract：:Negative feedback is known to enable biological and man-made systems to perform reliably in the face of uncertainties and disturbances. To date, synthetic biological feedback circuits have primarily relied upon protein-based, transcriptional regulation to control circuit output. Small RNAs (sRNAs) are non-coding RNA m...

journal_title：Nucleic acids research

authors： Kelly CL,Harris AWK,Steel H,Hancock EJ,Heap JT,Papachristodoulou A

更新日期：2018-10-12 00:00:00

abstract：:RNA polymerase II (pol II) utilizes a complex interaction network to select and incorporate correct nucleoside triphosphate (NTP) substrates with high efficiency and fidelity. Our previous 'synthetic nucleic acid substitution' strategy has been successfully applied in dissecting the function of nucleic acid moieties i...

journal_title：Nucleic acids research

authors： Hwang CS,Xu L,Wang W,Ulrich S,Zhang L,Chong J,Shin JH,Huang X,Kool ET,McKenna CE,Wang D

更新日期：2016-05-05 00:00:00

abstract：:Here we compare several methods for predicting oligonucleotide frequencies in 691 kb of Drosophila melanogaster DNA. As in previous work on Escherichia coli and Saccharomyces cerevisiae, a relatively simple equation based on tetranucleotide frequencies can be used in predicting frequencies of higher order oligonucleot...

journal_title：Nucleic acids research

authors： Cuticchia AJ,Ivarie R,Arnold J

更新日期：1992-07-25 00:00:00

abstract：:Reversible methylation of the N6 or N1 position of adenine in RNA has recently been shown to play significant roles in regulating the functions of RNA. RNA can also be alkylated upon exposure to endogenous and exogenous alkylating agents. Here we examined how regio-specific methylation at the hydrogen bonding edge of ...

journal_title：Nucleic acids research

authors： You C,Dai X,Wang Y

更新日期：2017-09-06 00:00:00

abstract：:We report, herein, a new class of RNAi trigger molecules based on the unconventional parallel hybridization of two oligonucleotide chains. We have prepared and studied several parallel stranded (ps) duplexes, in which the parallel orientation is achieved through incorporation of isoguanine and isocytosine to form reve...

journal_title：Nucleic acids research

authors： Habibian M,Yahyaee-Anzahaee M,Lucic M,Moroz E,Martín-Pintado N,Di Giovanni LD,Leroux JC,Hall J,González C,Damha MJ

更新日期：2018-02-28 00:00:00

abstract：:Benzo[a]pyrene is an important environmental mutagen and carcinogen. Its metabolism in cells yields the mutagenic, key ultimate carcinogen 7R,8S,9S,10R-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide, (+)-anti-BPDE, which reacts via its 10-position with N2-dG in DNA to form the adduct (+)-trans-anti-BPDE-N2-dG. To ga...

journal_title：Nucleic acids research

authors： Zhao B,Wang J,Geacintov NE,Wang Z

更新日期：2006-01-13 00:00:00

abstract：:The DNA-binding protein RdgC has been identified as an inhibitor of RecA-mediated homologous recombination in Escherichia coli. In Neisseria species, RdgC also has a role in virulence-associated antigenic variation. We have previously solved the crystal structure of the E. coli RdgC protein and shown it to form a toro...

journal_title：Nucleic acids research

authors： Briggs GS,Yu J,Mahdi AA,Lloyd RG

更新日期：2010-10-01 00:00:00

abstract：:31P NMR chemical shifts of the selected mono- and oligonucleotide derivatives, including the compounds with P-N, P-C, P-S bonds and phosphite nucleotide analogues have been presented. The influence of substituents upon 31P chemical shifts has been discussed. The concrete examples of 31P chemical shifts data applicatio...

journal_title：Nucleic acids research

authors： Lebedev AV,Rezvukhin AI

更新日期：1984-07-25 00:00:00

abstract：:Sequences of 3279 sequences of tRNA genes and tRNAs published up to December 1996 are included in the compilation. Alignment of the sequences, which is most compatible with the tRNA phylogeny and known three-dimensional structures of tRNA, is used. Sequences and references are available under http://www.uni-bayreuth. ...

journal_title：Nucleic acids research

authors： Sprinzl M,Horn C,Brown M,Ioudovitch A,Steinberg S

更新日期：1998-01-01 00:00:00