Abstract：
:Highly toxic DNA double-strand breaks (DSBs) readily trigger the DNA damage response (DDR) in cells, which delays cell cycle progression to ensure proper DSB repair. In Saccharomyces cerevisiae, mitotic S phase (20-30 min) is lengthened upon DNA damage. During meiosis, Spo11-induced DSB onset and repair lasts up to 5 h. We report that the NH2-terminal domain (NTD; residues 1-66) of Rad51 has dual functions for repairing DSBs during vegetative growth and meiosis. Firstly, Rad51-NTD exhibits autonomous expression-enhancing activity for high-level production of native Rad51 and when fused to exogenous β-galactosidase in vivo. Secondly, Rad51-NTD is an S/T-Q cluster domain (SCD) harboring three putative Mec1/Tel1 target sites. Mec1/Tel1-dependent phosphorylation antagonizes the proteasomal degradation pathway, increasing the half-life of Rad51 from ∼30 min to ≥180 min. Our results evidence a direct link between homologous recombination and DDR modulated by Rad51 homeostasis.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Woo TT,Chuang CN,Higashide M,Shinohara A,Wang TFdoi
10.1093/nar/gkaa587subject
Has Abstractpub_date
2020-09-04 00:00:00pages
8474-8489issue
15eissn
0305-1048issn
1362-4962pii
5870334journal_volume
48pub_type
杂志文章abstract：:Upon activation of the ERK and p38 MAPK pathways, the MSK1/2-mediated nucleosomal response, including H3 phosphorylation at serine 28 or 10, is coupled with the induction of immediate-early (IE) gene transcription. The outcome of this response, varying with the stimuli and cellular contexts, ranges from neoplastic tra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq030
更新日期：2010-06-01 00:00:00
abstract：:Human U6 small nuclear RNA (U6 snRNA), an abundant snRNA required for splicing of pre-mRNAs, contains several post-transcriptional modifications including a single m6A (N-6-methyladenosine) at position 43. This A-43 residue is critical for the function of U6 snRNA in splicing of pre-mRNAs. Yeast and plant U6 snRNAs al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.13.2421
更新日期：1995-07-11 00:00:00
abstract：:The surface accessibility of macromolecules plays a key role in modulating molecular recognition events. RNA is a complex and dynamic molecule involved in many aspects of gene expression. However, there are few experimental methods available to measure the accessible surface of RNA. Here, we investigate the accessible...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1062
更新日期：2008-03-01 00:00:00
abstract：:Several different computational problems have been solved using DNA as a medium. However, the DNA computations that have so far been carried out have examined a relatively small number of possible sequence solutions in order to find correct sequence solutions. We have encoded a search algorithm in DNA that required th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.22.5203
更新日期：1998-11-15 00:00:00
abstract：:Multifunctional proteins challenge the conventional 'one protein-one function' paradigm. Here we note apparent multifunctional proteins with nucleic acid partners, tabulating eight examples. We then focus on eight additional cases of transcription factors that bind double-stranded DNA with sequence specificity, but th...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkf512
更新日期：2002-10-01 00:00:00
abstract：:The final step in the maturation of paramyxoviruses, orthomyxoviruses and viruses of several other families, entails the budding of the viral nucleocapsid through the plasma membrane of the host cell. Many medically important viruses, such as influenza, parainfluenza, respiratory syncytial virus (RSV) and Ebola, can f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm246
更新日期：2007-01-01 00:00:00
abstract：:A growing number of whole genome sequencing projects, in combination with development of phylogenetic methods for reconstructing gene evolution, have provided us with a window into genomes that existed millions, and even billions, of years ago. Ancestral Genomes (http://ancestralgenomes.org) is a resource for comprehe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1009
更新日期：2019-01-08 00:00:00
abstract：:The Aspergillus Genome Database (AspGD) is an online genomics resource for researchers studying the genetics and molecular biology of the Aspergilli. AspGD combines high-quality manual curation of the experimental scientific literature examining the genetics and molecular biology of Aspergilli, cutting-edge comparativ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp751
更新日期：2010-01-01 00:00:00
abstract：:RegulonDB, first published 20 years ago, is a comprehensive electronic resource about regulation of transcription initiation of Escherichia coli K-12 with decades of knowledge from classic molecular biology experiments, and recently also from high-throughput genomic methodologies. We curated the literature to keep Reg...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1077
更新日期：2019-01-08 00:00:00
abstract：:Although Dicer is essential for general microRNA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent. Instead, its short pre-miRNA hairpin is 'sliced' by Ago2, then 3'-resected into mature miRNAs. Here, we show that Drosophila cells and animals generate functional small RNAs from mir-451-type precursors. Howev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1038
更新日期：2014-02-01 00:00:00
abstract：:Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be extremely low in these samples and ultra-sensitive methods are required for their detection. Here, we report an extremely simple and fast...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw224
更新日期：2016-06-20 00:00:00
abstract：:Multiple regulatory modules contribute to the complex expression programs realized by many loci. Although long thought of as isolated components, recent studies demonstrate that such regulatory sequences can physically associate with promoters and with each other and may localize to specific sub-nuclear transcription ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1160
更新日期：2011-04-01 00:00:00
abstract：:TropGeneDB (http://tropgenedb.cirad.fr) was created to store genetic, molecular and phenotypic data on tropical crop species. The most common data stored in TropGeneDB are molecular markers, quantitative trait loci, genetic and physical maps, genetic diversity, phenotypic diversity studies and information on genetic r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1105
更新日期：2013-01-01 00:00:00
abstract：:To determine whether methylation changes in specific DNA sequences of the albumin and AFP genes are implicated in the modulation of transcriptional activity during rat liver development and neoplasia we have analysed the methylation pattern of C-C-G-G sequences within these genes in DNA isolated from fetal and adult h...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.13.4335
更新日期：1983-07-11 00:00:00
abstract：:Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type cl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.6.1187
更新日期：1980-03-25 00:00:00
abstract：:T7 RNA polymerase promoters consist of a highly conserved 23 base-pair sequence that spans the site of the initiation of transcription (+1) and extends from -17 to +6. To determine the bases within the T7 consensus promoter that are essential for promoter function a library of mutant T7 promoters was constructed, and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.10.2517
更新日期：1992-05-25 00:00:00
abstract：:We have developed a novel method for estimating the parameters of hidden Markov models for gene finding in newly sequenced species. Our approach does not rely on curated training data sets, but instead uses extrinsic evidence (including paired-end ditags that have not been used in gene finding previously) and iterativ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp052
更新日期：2009-04-01 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) that have no protein-coding capacity make up a large proportion of the transcriptome of various species. Many lncRNAs are expressed within the animal central nervous system in spatial- and temporal-specific patterns, indicating that lncRNAs play important roles in cellular processes, neu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks943
更新日期：2012-12-01 00:00:00
abstract：:Modified bases, such as O6-methylguanines, are produced in cells exposed to alkylating agents and cause apoptosis. In human cells treated with N-methyl-N-nitrosourea, we detected a protein complex composed of MutSalpha, MutLalpha and PCNA on damaged DNA by immunoprecipitation method using chromatin extracts, in which ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki878
更新日期：2005-10-04 00:00:00
abstract：:We have sequenced the 480 base pair (bp) repeating unit of the 5S RNA genes of the Dipteran fly Calliphora erythrocephala and compared this sequence to the three known 5S RNA gene sequences from the Dipteran Genus Drosophila (1,2). A striking series of five perfectly conserved homologies identically positioned within ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.21.8193
更新日期：1984-11-12 00:00:00
abstract：:The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq318
更新日期：2010-07-01 00:00:00
abstract：:The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.21.7607
更新日期：1985-11-11 00:00:00
abstract：:The endonuclease activity within the influenza virus cap-snatching process is a proven therapeutic target. The anti-influenza drug baloxavir is highly effective, but is associated with resistance mutations that threaten its clinical efficacy. The endonuclease resides within the N-terminal domain of the PA subunit (PAN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1294
更新日期：2021-01-19 00:00:00
abstract：:The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multiple aspects of mitochondrial RNA metabolism. Here, we investigate the role of TbDSS-1 in RNA processing and surveillance by analyzing 12S rRNA processing intermediates in TbDSS-1 RNAi cells. RNA fragments corresponding to leader sequence upst...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm690
更新日期：2008-01-01 00:00:00
abstract：:A 15-residue sequence motif has been found in many polymerases from various species and involving DNA and RNA dependence and product. The motif is characterized by a Tyr-Gly-Asp-(Thr)-Asp core flanked by hydrophobic spans five residues in length. An mRNA maturase segment is also suggested to display the motif pattern....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.21.9909
更新日期：1988-11-11 00:00:00
abstract：:Rapid and transient activation of both c-fos transcription and mRNA accumulation occurs when resting CCL39 hamster fibroblasts are serum-stimulated to grow. By using several combinations of serum and cycloheximide, a protein synthesis inhibitor, we showed that: i) addition of cycloheximide to resting cell elicits an i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.14.5657
更新日期：1987-07-24 00:00:00
abstract：:The extrusion kinetics of two cruciforms derived from unrelated DNA sequences differ markedly. Kinetic barriers exist for both reactions, necessitating elevated temperatures before extrusion proceeds at measureable speeds, but the dependence upon temperature and ionic strength is quite different for the two sequences....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.5.1443
更新日期：1985-03-11 00:00:00
abstract：:We recently reported that the antitumor triazoloacridone, compound C-1305, is a topoisomerase II poison with unusual properties. In this study we characterize the DNA interactions of C-1305 in vitro, in comparison with other topoisomerase II inhibitors. Our results show that C-1305 binds to DNA by intercalation and po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki904
更新日期：2005-10-27 00:00:00
abstract：:It is known that DNA curvature plays a certain role in gene regulation. The distribution of curved DNA in promoter regions is evolutionarily preserved, and it is mainly determined by temperature of habitat. However, very little is known on the distribution of DNA curvature in termination sites. Our main objective was ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl230
更新日期：2006-05-05 00:00:00
abstract：:The human transcription factor EF-1A binds to the purine-rich E1A core enhancer sequence in the adenovirus E1A and E4 and polyomavirus enhancer regions. The consensus binding site for EF-1A resembles that of members of the ets domain protein family. EF-1A activation of transcription requires a dimeric binding site. An...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.24.6555
更新日期：1992-12-25 00:00:00
