Abstract:
:An alpha-neo-endorphin (alpha NE) gene, which we previously synthesized chemically and inserted into E. coli beta-galactosidase gene of pK013 plasmid, has been excised and fused to E. coli alkaline phosphatase (APase) gene. One of the transformants was named E15/pA alpha NE1. Under the APase gene regulation, APase-alpha NE chimeric protein was expressed at 1.3 X 10(6) molecules per cell, and accounted for about 60% of total cellular proteins. The HPLC pattern of CNBr treated E15/pA alpha NE1 was very simple reflecting the high content of the chimeric protein and low numbers of methionine residues in it. A series of genes encoding APase-alpha NE chimeric proteins in which 30 to 94 C-terminal amino acid residues were replaced by (met)-alpha NE, was cloned in E. coli. Transportation of the chimeric proteins to periplasmic space was studied. All chimeric proteins were apparently processed by signal peptidase but few, if any, was transported to the periplasmic space.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ohsuye K,Nomura M,Tanaka S,Kubota I,Nakazato H,Shinagawa H,Nakata A,Noguchi Tdoi
10.1093/nar/11.5.1283subject
Has Abstractpub_date
1983-03-11 00:00:00pages
1283-94issue
5eissn
0305-1048issn
1362-4962journal_volume
11pub_type
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