Abstract:
:The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nuclear structure. Single strand specific digestion by nuclease S1 or staphylococcal nuclease at low concentrations caused a release of about 30% of the pulse label, without significantly affecting the attachment of randomly labelled DNA. The released material had a low sedimentation coefficient and contained most of the Okasaki fragments. The remaining pulse label was less accessible to further digestion by double strand specific nuclease activity than the bulk DNA. The results suggest that an attachment of the replication fork to the nuclear structure occurs at sites behind but close to the branch point.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Dijkwel PA,Mullenders LH,Wanka Fdoi
10.1093/nar/6.1.219subject
Has Abstractpub_date
1979-01-01 00:00:00pages
219-30issue
1eissn
0305-1048issn
1362-4962journal_volume
6pub_type
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