Abstract:
:The precursor to the Euglena light harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) is a polyprotein containing multiple copies of LHCPII covalently joined by a decapeptide linker. cDNA and genomic clones encoding the 5' and 3' end of a 6.6 kb LHCPII mRNA were sequenced. A 3.1 kb genomic region encoding 1.05 kb of the 5' end of LHCPII mRNA contains 4 introns. A 7.6 kb genomic region encoding 3.3 kb of the 3' end of LHCPII mRNA contains 10 introns. The 5' and 3' ends of the 14 identified Euglena introns lacked the conserved dinucleotides (5'-GT and AG-3') found at the termini of virtually every characterized nuclear pre-mRNA intron. A common consensus splice site selection sequence could not be identified. The Euglena introns do not have the structural characteristics of group I and group II introns. The only structural feature common to all Euglena introns was the ability of short stretches of nucleotides at the 5' and 3' ends of the introns to base pair, forming a stable stem-loop with the 5' and 3' splice site juxtaposed for splicing but displaced by 2 nucleotides. The 26 nucleotide sequence at the 5' end of LHCPII mRNA is absent from the genomic sequence and identical to the 5' end of one of the small Euglena SL-RNAs indicating that it is post-transcriptionally added by trans-splicing.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Muchhal US,Schwartzbach SDdoi
10.1093/nar/22.25.5737subject
Has Abstractpub_date
1994-12-25 00:00:00pages
5737-44issue
25eissn
0305-1048issn
1362-4962journal_volume
22pub_type
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