A new method for predicting signal sequence cleavage sites.

abstract：:Pax-5, a member of the paired domain family of transcription factors, is a key regulator of B lymphocyte-specific transcription and differentiation. A major target of Pax-5-mediated activation is the mb-1 gene, which encodes the essential transmembrane signaling protein Ig-alpha. Pax-5 recruits three members of the Et...

journal_title：Nucleic acids research

authors： Maier H,Ostraat R,Parenti S,Fitzsimmons D,Abraham LJ,Garvie CW,Hagman J

更新日期：2003-10-01 00:00:00

abstract：:The Functional Single Nucleotide Polymorphism (F-SNP) database integrates information obtained from 16 bioinformatics tools and databases about the functional effects of SNPs. These effects are predicted and indicated at the splicing, transcriptional, translational and post-translational level. As such, the database h...

journal_title：Nucleic acids research

authors： Lee PH,Shatkay H

更新日期：2008-01-01 00:00:00

abstract：:Synthetic DNA fragments were constructed to determine the effect of G tracts, in conjunction with periodically spaced A tracts, on DNA bends. Relative length measurements showed that the G tracts spaced at the half helical turn enhanced the DNA bend. When the G tract was interrupted with a thymine or shortened to one ...

journal_title：Nucleic acids research

authors： Milton DL,Casper ML,Wills NM,Gesteland RF

更新日期：1990-02-25 00:00:00

abstract：:Post-transcriptional regulation and the formation of mRNA 3' ends are crucial for gene expression in eukaryotes. Interspecies conservation of many sequences within 3'UTRs reveals selective constraint due to similar function. To study the pattern of conservation within 3'UTRs, we compiled and aligned 50 sets of complet...

journal_title：Nucleic acids research

authors： Shabalina SA,Ogurtsov AY,Lipman DJ,Kondrashov AS

更新日期：2003-09-15 00:00:00

abstract：:The BRENDA enzyme database (https://www.brenda-enzymes.org), established in 1987, has evolved into the main collection of functional enzyme and metabolism data. In 2018, BRENDA was selected as an ELIXIR Core Data Resource. BRENDA provides reliable data, continuous curation and updates of classified enzymes, and the in...

journal_title：Nucleic acids research

authors： Chang A,Jeske L,Ulbrich S,Hofmann J,Koblitz J,Schomburg I,Neumann-Schaal M,Jahn D,Schomburg D

更新日期：2021-01-08 00:00:00

abstract：:Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...

journal_title：Nucleic acids research

authors： Sinclair JH,Bryant LA

更新日期：1987-11-25 00:00:00

abstract：:The editing function of aminoacyl-tRNA synthetases (aaRSs) is indispensible for formation of the correct aminoacyl-tRNAs. Editing deficiency may lead to growth inhibition and the pathogenesis of various diseases. Herein, we confirmed that norvaline (Nva) but not isoleucine or valine is the major threat to the editing ...

journal_title：Nucleic acids research

authors： Ji QQ,Fang ZP,Ye Q,Chi CW,Wang ED

更新日期：2017-07-07 00:00:00

abstract：:Chromatin modulation at various cis-acting elements is critical for V(D)J recombination during T and B cell development. MARbeta, a matrix-associated region (MAR) located upstream of the T cell receptor beta (TCRbeta) enhancer (Ebeta), serves a crucial role in silencing Ebeta-mediated TCR activation. By DNaseI hyperse...

journal_title：Nucleic acids research

authors： Kaul-Ghanekar R,Jalota A,Pavithra L,Tucker P,Chattopadhyay S

更新日期：2004-09-15 00:00:00

abstract：:Many genetic manipulations are limited by difficulty in obtaining adequate levels of protein expression. Bioinformatic and experimental studies have identified nucleotide sequence features that may increase expression, however it is difficult to assess the relative influence of these features. Zebrafish embryos are ra...

journal_title：Nucleic acids research

authors： Horstick EJ,Jordan DC,Bergeron SA,Tabor KM,Serpe M,Feldman B,Burgess HA

更新日期：2015-04-20 00:00:00

abstract：:Furvina®, also denominated G1 (MW 297), is a synthetic nitrovinylfuran [2-bromo-5-(2-bromo-2-nitrovinyl)-furan] antibiotic with a broad antimicrobial spectrum. An ointment (Dermofural®) containing G1 as the only active principle is currently marketed in Cuba and successfully used to treat dermatological infections. He...

journal_title：Nucleic acids research

authors： Fabbretti A,Brandi L,Petrelli D,Pon CL,Castañedo NR,Medina R,Gualerzi CO

更新日期：2012-11-01 00:00:00

abstract：:Unwanted side effects of drugs are a burden on patients and a severe impediment in the development of new drugs. At the same time, adverse drug reactions (ADRs) recorded during clinical trials are an important source of human phenotypic data. It is therefore essential to combine data on drugs, targets and side effects...

journal_title：Nucleic acids research

authors： Kuhn M,Letunic I,Jensen LJ,Bork P

更新日期：2016-01-04 00:00:00

abstract：:The Gene Expression Atlas (http://www.ebi.ac.uk/gxa) is an added-value database providing information about gene expression in different cell types, organism parts, developmental stages, disease states, sample treatments and other biological/experimental conditions. The content of this database derives from curation, ...

journal_title：Nucleic acids research

authors： Kapushesky M,Emam I,Holloway E,Kurnosov P,Zorin A,Malone J,Rustici G,Williams E,Parkinson H,Brazma A

更新日期：2010-01-01 00:00:00

abstract：:The oncogene BMI1 encodes a polycomb group transcription factor that is required for embryonic development and self-renewal of stem cells. Despite these important functions little is known about the regulation of BMI1 expression. A cDNA microarray based search for target genes of E2F-1 in neuroblastoma cells expressin...

journal_title：Nucleic acids research

authors： Nowak K,Kerl K,Fehr D,Kramps C,Gessner C,Killmer K,Samans B,Berwanger B,Christiansen H,Lutz W

更新日期：2006-03-31 00:00:00

abstract：:Hydroxylapatite chromatographies of mitochondrial and total enzymes from a rho+ yeast, or from the related rho degrees mitochondrial DNA-less mutant, show the occurrence in the mitochondrial enzyme of one Phe-, one Met-, one Leu-tRNA synthetase peak which elutes distinctly from the cytoplasmic counterpart and charges ...

journal_title：Nucleic acids research

authors： Schneller JM,Schneller C,Martin R,Stahl AJ

更新日期：1976-05-01 00:00:00

abstract：:Alternative splicing constitutes a major mechanism creating protein diversity in humans. This diversity can result from the alternative skipping of entire exons or by alternative selection of the 5' or 3' splice sites that define the exon boundaries. In this study, we analyze the sequence and evolutionary characterist...

journal_title：Nucleic acids research

authors： Akerman M,Mandel-Gutfreund Y

更新日期：2007-01-01 00:00:00

abstract：:The vertebrate genome is a mosaic of regions differing dramatically in their G + C content. Those regions with a high G + C content contain the expected number of CpG dinucleotides and we propose that following methylation these have been protected from deamination by the increased stability of the surrounding DNA dup...

journal_title：Nucleic acids research

authors： Adams RL,Eason R

更新日期：1984-07-25 00:00:00

abstract：:Friedreich ataxia (FRDA) is caused by hyperexpansion of GAA*TTC repeats located in the first intron of the FXN gene, which inhibits transcription leading to the deficiency of frataxin. The FXN gene is an excellent target for therapeutic intervention since (i) 98% of patients carry the same type of mutation, (ii) the m...

journal_title：Nucleic acids research

authors： Soragni E,Herman D,Dent SY,Gottesfeld JM,Wells RD,Napierala M

更新日期：2008-11-01 00:00:00

abstract：:Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...

journal_title：Nucleic acids research

authors： Kang JJ,Watson RM,Fisher ME,Higuchi R,Gelfand DH,Holland MJ

更新日期：2000-01-15 00:00:00

abstract：:Chromatin assembly factor 1 (CAF-1) is a histone H3-H4 chaperone that deposits newly synthesized histone (H3-H4)2 tetramers during replication-coupled nucleosome assembly. However, how CAF-1 functions in this process is not yet well understood. Here, we report the crystal structure of C terminus of Cac1 (Cac1C), a sub...

journal_title：Nucleic acids research

authors： Zhang K,Gao Y,Li J,Burgess R,Han J,Liang H,Zhang Z,Liu Y

更新日期：2016-06-20 00:00:00

abstract：:The Rhizobium trifolii nod genes required for host-specific nodulation of clovers are located on 14 kb of Sym (symbiotic) plasmid DNA. Analysis of the nucleotide sequence of a 3.7 kb portion of this region has revealed open reading frames corresponding to the nodABCDEF genes. A DNA sequencing technique, using primer e...

journal_title：Nucleic acids research

authors： Schofield PR,Watson JM

更新日期：1986-04-11 00:00:00

abstract：:In this study, we show that silencing of CITED2 using small-hairpin RNA (shCITED2) induced DNA damage and reduction of ERCC1 gene expression in HEK293, HeLa and H1299 cells, even in the absence of cisplatin. In contrast, ectopic expression of ERCC1 significantly reduced intrinsic and induced DNA damage levels, and res...

journal_title：Nucleic acids research

authors： Liu YC,Chang PY,Chao CC

更新日期：2015-12-15 00:00:00

abstract：:The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional activity of, and the protein-DNA interactions that occur within, the proximal region of the human beta-actin promoter. Eff...

journal_title：Nucleic acids research

authors： Danilition SL,Frederickson RM,Taylor CY,Miyamoto NG

更新日期：1991-12-25 00:00:00

abstract：:Bacterial Rho-independent terminators (RITs) are important genomic landmarks involved in gene regulation and terminating gene expression. In this investigation we present RNIE, a probabilistic approach for predicting RITs. The method is based upon covariance models which have been known for many years to be the most a...

journal_title：Nucleic acids research

authors： Gardner PP,Barquist L,Bateman A,Nawrocki EP,Weinberg Z

更新日期：2011-08-01 00:00:00

abstract：:Accurate T-cell epitope prediction is a principal objective of computational vaccinology. As a service to the immunology and vaccinology communities at large, we have implemented, as a server on the World Wide Web, a partial least squares-based multivariate statistical approach to the quantitative prediction of peptid...

journal_title：Nucleic acids research

authors： Guan P,Doytchinova IA,Zygouri C,Flower DR

更新日期：2003-07-01 00:00:00

abstract：:Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNA-like (C3'-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself. We performed molecular dynamics simulations on five isosequential duplexes (LNA-DNA, LNA-LNA, LNA-R...

journal_title：Nucleic acids research

authors： Pande V,Nilsson L

更新日期：2008-03-01 00:00:00

abstract：:SynergyFinder (https://synergyfinder.fimm.fi) is a stand-alone web-application for interactive analysis and visualization of drug combination screening data. Since its first release in 2017, SynergyFinder has become a widely used web-tool both for the discovery of novel synergistic drug combinations in pre-clinical mo...

journal_title：Nucleic acids research

authors： Ianevski A,Giri AK,Aittokallio T

更新日期：2020-07-02 00:00:00

abstract：:The development of biosensors based on DNA hybridization requires a more precise knowledge of the thermodynamics of the hybridization at a solid interface. In particular, the selectivity of hybridization can be affected by a lot of parameters such as the single-strand (ss)DNA density, the pH, the ionic strength or the...

journal_title：Nucleic acids research

authors： Meunier-Prest R,Raveau S,Finot E,Legay G,Cherkaoui-Malki M,Latruffe N

更新日期：2003-12-01 00:00:00

abstract：:Analyses of secondary structures proposed for ribosomal RNA's show that, of the different kinds of base pairs directly adjoining the ends of postulated double-helical regions, only A-G with A at the 5' end significantly exceeds the number expected for a random base distribution. An A(syn)-G(trans) hydrogen-bonded base...

journal_title：Nucleic acids research

authors： Traub W,Sussman JL

更新日期：1982-04-24 00:00:00

abstract：:The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in...

journal_title：Nucleic acids research

authors： Hansen-Hagge TE,Trefzer U,zu Reventlow AS,Kaltoft K,Sterry W

更新日期：2001-02-15 00:00:00

abstract：:The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted cod...

journal_title：Nucleic acids research

authors： Azuma Y,Yamagishi M,Ueshima R,Ishihama A

更新日期：1991-02-11 00:00:00