Does distance matter? Variations in alternative 3' splicing regulation.

abstract：:Nuclear DNA from liver cells of the kangaroo rat species Dipodomysordii was fractionated and characterized with the aid of buoyant density gradients in neutral and alkaline CsCl and in Ag(+)-Cs(2)SO(4). More than one-half of the DNA was present in three density satellites, a greater proportion than in any other specie...

journal_title：Nucleic acids research

authors： Hatch FT,Mazrimas JA

更新日期：1974-04-01 00:00:00

abstract：:Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5' flaps. It is also involved in the maintenance of telomeres. DNA2 is a gene conserved in eukaryotes, and a putative human ortholog of yeast DNA2 (ScDNA2) ...

journal_title：Nucleic acids research

authors： Masuda-Sasa T,Imamura O,Campbell JL

更新日期：2006-04-04 00:00:00

abstract：:The complete nucleotide sequences of the various precursor 5S RNA species occurring in Bacillus licheniformis have been elucidated. The B. licheniformis precursors contain a 5'-precursor-specific segment of 95 nucleotides which is four times as long as the corresponding segment of the p5S RNAs from the closely related...

journal_title：Nucleic acids research

authors： Stiekema WJ,de Leede-Twente R,Raué HA,Planta RJ

更新日期：1980-10-10 00:00:00

abstract：:The MF alpha 1 gene of Saccharomyces cerevisiae is an alpha specific gene whose expression is regulated by two upstream activation sites (UASMF alpha 1S), which are the binding sites for activator proteins, MAT alpha 1 and MCM1. UASMF alpha 1 elements act in a synergistic fashion; lack of either element causes reduced...

journal_title：Nucleic acids research

authors： Inokuchi K,Nakayama A

更新日期：1991-06-11 00:00:00

abstract：:TAP/hNXF1 is a key factor that mediates general cellular mRNA export from the nucleus, and its orthologs are structurally and functionally conserved from yeast to humans. Metazoans encode additional proteins that share homology and domain organization with TAP/hNXF1, suggesting their participation in mRNA metabolism; ...

journal_title：Nucleic acids research

authors： Tan W,Zolotukhin AS,Tretyakova I,Bear J,Lindtner S,Smulevitch SV,Felber BK

更新日期：2005-07-13 00:00:00

abstract：:DNA damage-induced cell cycle checkpoints serve as surveillance mechanisms to maintain genomic stability, and are regulated by ATM/ATR-mediated signaling pathways that are conserved from yeast to humans. Trypanosoma brucei, an early divergent microbial eukaryote, lacks key components of the conventional DNA damage-ind...

journal_title：Nucleic acids research

authors： Zhou Q,Pham KTM,Hu H,Kurasawa Y,Li Z

更新日期：2019-09-05 00:00:00

abstract：:An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its applicat...

journal_title：Nucleic acids research

authors： Burckhardt J,Telford J,Birnstiel ML

更新日期：1979-07-11 00:00:00

abstract：:For efficient DNA cleavage, the Type III restriction endonuclease EcoP15I communicates with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of methylation (Mod) and restriction (Res) subunits forming a multifunctional enzyme complex able to methylate or to cleave DNA. In this stu...

journal_title：Nucleic acids research

authors： Wyszomirski KH,Curth U,Alves J,Mackeldanz P,Möncke-Buchner E,Schutkowski M,Krüger DH,Reuter M

更新日期：2012-04-01 00:00:00

abstract：:MethBank (http://bigd.big.ac.cn/methbank) is a database that integrates high-quality DNA methylomes across a variety of species and provides an interactive browser for visualization of methylation data. Here, we present an updated implementation of MethBank (version 3.0) by incorporating more DNA methylomes from multi...

journal_title：Nucleic acids research

authors： Li R,Liang F,Li M,Zou D,Sun S,Zhao Y,Zhao W,Bao Y,Xiao J,Zhang Z

更新日期：2018-01-04 00:00:00

abstract：:The gene for the thymidine kinase (TK) of Herpes simplex virus type 1 (HSV-1) is located in the KpnI m and BamHI p fragments of the genome (Wigler et al., Cell 11, 223-232 (1977)). These fragments have been inserted into the EcoRI and BamHI sites, respectively, of plasmid pBR322, and propagated in E.coli. The TK gene ...

journal_title：Nucleic acids research

authors： Wilkie NM,Clements JB,Boll W,Mantei N,Lonsdale D,Weissmann C

更新日期：1979-10-25 00:00:00

abstract：:Long non-coding RNAs (lncRNAs) that have no protein-coding capacity make up a large proportion of the transcriptome of various species. Many lncRNAs are expressed within the animal central nervous system in spatial- and temporal-specific patterns, indicating that lncRNAs play important roles in cellular processes, neu...

journal_title：Nucleic acids research

authors： Li M,Wen S,Guo X,Bai B,Gong Z,Liu X,Wang Y,Zhou Y,Chen X,Liu L,Chen R

更新日期：2012-12-01 00:00:00

abstract：:Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFbeta superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFbeta on Pax6 expression was studied in t...

journal_title：Nucleic acids research

authors： Grocott T,Frost V,Maillard M,Johansen T,Wheeler GN,Dawes LJ,Wormstone IM,Chantry A

更新日期：2007-01-01 00:00:00

abstract：:Complete genomes of three closely related Gram-positive bacteria Streptococcus pyogenes, Streptococcus pneumoniae and Lactococcus lactis are analyzed for abundances of short DNA sequence motifs (frequent words). The character and extent of frequent words are strikingly different among these genomes. The frequent words...

journal_title：Nucleic acids research

authors： Mrázek J,Gaynon LH,Karlin S

更新日期：2002-10-01 00:00:00

abstract：:Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the...

journal_title：Nucleic acids research

authors： Jha V,Roy B,Jahagirdar D,McNutt ZA,Shatoff EA,Boleratz BL,Watkins DE,Bundschuh R,Basu K,Ortega J,Fredrick K

更新日期：2021-01-11 00:00:00

abstract：:Targeted modulation of gene expression represents a valuable approach to understand the mechanisms governing gene regulation. In a therapeutic context, it can be exploited to selectively modify the aberrant expression of a disease-causing gene or to provide the target cells with a new function. Here, we have establish...

journal_title：Nucleic acids research

authors： Mlambo T,Nitsch S,Hildenbeutel M,Romito M,Müller M,Bossen C,Diederichs S,Cornu TI,Cathomen T,Mussolino C

更新日期：2018-05-18 00:00:00

abstract：:A truly universal nucleobase enables a host of novel applications such as simplified templates for PCR primers, randomized sequencing and DNA based devices. A universal base must pair indiscriminately to each of the canonical bases with little or preferably no destabilization of the overall duplex. In reality, many ca...

journal_title：Nucleic acids research

authors： Spring-Connell AM,Evich MG,Debelak H,Seela F,Germann MW

更新日期：2016-10-14 00:00:00

abstract：:Highly repetitive Hind III restriction fragments of 0.72-0.76 KBP from total Xenopus laevis genomic DNA are organized in a tandem like arrangement. Cloning of these fragments in pBR 322 with subsequent restriction site mapping and nucleotide sequence analysis of some selected clones showed two different types of seque...

journal_title：Nucleic acids research

authors： Meyerhof W,Tappeser B,Korge E,Knöchel W

更新日期：1983-10-25 00:00:00

abstract：:By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of Streptomyces griseus. O...

journal_title：Nucleic acids research

authors： Kobayashi T,Takao M,Oikawa A,Yasui A

更新日期：1989-06-26 00:00:00

abstract：:Splicing of mammalian introns requires that the intron possess at least 80 nucleotides. This length requirement presumably reflects the constraints of accommodating multiple snRNPs simultaneously in the same intron. In the free-living nematode, C. elegans, introns typically are 45 to 55 nucleotides in length. In this ...

journal_title：Nucleic acids research

authors： Ogg SC,Anderson P,Wickens MP

更新日期：1990-01-11 00:00:00

abstract：:SPRING (http://algorithm.cs.nthu.edu.tw/tools/SPRING/) is a tool for the analysis of genome rearrangement between two chromosomal genomes using reversals and/or block-interchanges. SPRING takes two or more chromosomes as its input and then computes a minimum series of reversals and/or block-interchanges between any tw...

journal_title：Nucleic acids research

authors： Lin YC,Lu CL,Liu YC,Tang CY

更新日期：2006-07-01 00:00:00

abstract：:Molecular models of six anthracycline antibiotics and their complexes with 32 distinct DNA octamer sequences were created and analyzed using HINT (Hydropathic INTeractions) to describe binding. The averaged binding scores were then used to calculate the free energies of binding for comparison with experimentally deter...

journal_title：Nucleic acids research

authors： Cashman DJ,Scarsdale JN,Kellogg GE

更新日期：2003-08-01 00:00:00

abstract：:Camptothecin (CPT), a plant alkaloid with antitumor activity, is a specific inhibitor of eukaryotic DNA topoisomerase I. We have previously isolated and characterized a CPT-resistant topoisomerase I isolated from a CPT-resistant human leukemia cell line, CPT-K5. cDNA clones of topoisomerase I were isolated from the CP...

journal_title：Nucleic acids research

authors： Tamura H,Kohchi C,Yamada R,Ikeda T,Koiwai O,Patterson E,Keene JD,Okada K,Kjeldsen E,Nishikawa K

更新日期：1991-01-11 00:00:00

abstract：:The Moloney murine leukemia virus (MLV) is a prototype gammaretrovirus requiring nuclear disassembly before DNA integration. In the nucleus, integration site selection towards promoter/enhancer elements is mediated by the host factor bromo- and extraterminal domain (BET) proteins (bromodomain (Brd) proteins 2, 3 and 4...

journal_title：Nucleic acids research

authors： Borrenberghs D,Zurnic I,De Wit F,Acke A,Dirix L,Cereseto A,Debyser Z,Hendrix J

更新日期：2019-02-20 00:00:00

abstract：:Mouse embryo fibroblasts (MEFs) are convenient sources for biochemical studies when cell number in mouse embryos is limiting. To derive the imprinting signature of MEFs and potentially detect novel imprinted genes we performed strand- and allele-specific RNA deep sequencing. We used sequenom allelotyping in embryo and...

journal_title：Nucleic acids research

authors： Tran DA,Bai AY,Singh P,Wu X,Szabó PE

更新日期：2014-02-01 00:00:00

abstract：:Mutations in human mitochondrial DNA are often associated with incurable human neuromuscular diseases. Among these mutations, an important number have been identified in tRNA genes, including 29 in the gene MT-TL1 coding for the tRNA(Leu(UUR)). The m.3243A>G mutation was described as the major cause of the MELAS syndr...

journal_title：Nucleic acids research

authors： Karicheva OZ,Kolesnikova OA,Schirtz T,Vysokikh MY,Mager-Heckel AM,Lombès A,Boucheham A,Krasheninnikov IA,Martin RP,Entelis N,Tarassov I

更新日期：2011-10-01 00:00:00

abstract：:The pyrrole-imidazole (Py-Im) triamide-cyclopropa pyrroloindole (CPI) conjugates ImPyImLDu86 (7) and ImImPyLDu86 (14) were synthesized and their alkylating activities and inhibitory effects on DNA hydrolysis by restriction endonucleases were examined. Sequencing gel analysis demonstrated that conjugates 7 and 14 speci...

journal_title：Nucleic acids research

authors： Fujimoto K,Iida H,Kawakami M,Bando T,Tao ZF,Sugiyama H

更新日期：2002-09-01 00:00:00

abstract：:RNA polymerase (RNAP) is a major target of gene regulation. Thermus thermophilus bacteriophage P23-45 encodes two RNAP binding proteins, gp39 and gp76, which shut off host gene transcription while allowing orderly transcription of phage genes. We previously reported the structure of the T. thermophilus RNAP•σA holoenz...

journal_title：Nucleic acids research

authors： Ooi WY,Murayama Y,Mekler V,Minakhin L,Severinov K,Yokoyama S,Sekine SI

更新日期：2018-01-09 00:00:00

abstract：:To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...

journal_title：Nucleic acids research

authors： Heuchel R,Matthias P,Schaffner W

更新日期：1989-11-25 00:00:00

abstract：:DNA restriction fragments located 5' to the human c-myc gene display anomalous electrophoretic mobility on polyacrylamide gels. Computer modeling of the c-myc flanking DNA suggests that the slow-moving DNA fragments spanning nucleotides -1690 to -1054 (relative to c-myc promoter P1) and -718 to -452 form large left ha...

journal_title：Nucleic acids research

authors： Kumar S,Leffak M

更新日期：1989-04-11 00:00:00

abstract：:Integration of foreign genes into plant genomes by the Agrobacterium T-DNA transfer system has been considered to occur at random. It has been speculated that the chromosomal structure of the integration site might affect the expression pattern of the introduced genes. To gain insight into the molecular structure of T...

journal_title：Nucleic acids research

authors： Dietz A,Kay V,Schlake T,Landsmann J,Bode J

更新日期：1994-07-25 00:00:00