Abstract:
:The oncogene BMI1 encodes a polycomb group transcription factor that is required for embryonic development and self-renewal of stem cells. Despite these important functions little is known about the regulation of BMI1 expression. A cDNA microarray based search for target genes of E2F-1 in neuroblastoma cells expressing a 4-OHT-regulated E2F-1-ER fusion protein identified many hitherto unknown E2F-1 regulated genes. A total of 10% of these genes, including BMI1, encode proteins that function primarily in the regulation of gene expression. The BMI1 promoter contains a putative E2F binding site that was required for the activation of a BMI1 promoter-dependent reporter construct by E2F-1. Chromatin immunoprecipitation revealed 4-OHT-dependent binding of E2F-1-ER and binding of endogenous E2F-1 to the BMI1 promoter in tumor cells. We have previously shown activation of the oncogene MYCN by E2F. Thus, in neuroblastomas deregulated E2F-1 can activate two oncogenes, MYCN and BMI1 that are known to co-operate in tumor formation. Consistent with a role of Bmi1 in neuroblastoma tumorigenesis we found strong Bmi1 expression in primary neuroblastomas. Our results reveal a novel link between E2F and polycomb transcription factors and suggest a role of Bmi1 in neuroblastomas.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Nowak K,Kerl K,Fehr D,Kramps C,Gessner C,Killmer K,Samans B,Berwanger B,Christiansen H,Lutz Wdoi
10.1093/nar/gkl119keywords:
subject
Has Abstractpub_date
2006-03-31 00:00:00pages
1745-54issue
6eissn
0305-1048issn
1362-4962pii
34/6/1745journal_volume
34pub_type
杂志文章abstract::Advances in genomic sequencing have enormous potential to revolutionize personalized medicine, however distinguishing disease-causing from benign variants remains a challenge. The increasing number of human genome and exome sequences available has revealed areas where unfavourable variation is removed through purifyin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz457
更新日期:2019-07-02 00:00:00
abstract::Nucleobase analogs 5-methylisocytosine ((Me)isoC) and isoguanine (isoG) form a non-natural base pair in duplex nucleic acids with base pairing specificity orthogonal to the natural nucleobase pairs. Sequencing reactions were conducted with oligodeoxyribonucleotides (ODNs) containing d(Me)isoC and disoG using modified ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki628
更新日期:2005-06-02 00:00:00
abstract::A non-redundant database of nuclear, protein-encoding, genomic DNA sequences highlighting nuclear pre-mRNA introns was constructed using information contained in the SWISS-PROT and GenBank sequence databases. This Intron DataBase (IDB) contains information about (i) introns (including nucleotide sequence, location, ph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.181
更新日期:2000-01-01 00:00:00
abstract::We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.10.e45
更新日期:2002-05-15 00:00:00
abstract::The CRT10 gene was identified through screening of the Saccharomyces cerevisiae deletion library for hydroxyurea (HU) resistance. CRT10 encodes a putative 957 amino acid, 110 kDa protein with a leucine repeat and a WD40 repeat near the N-terminus. Deletion of CRT10 resulted in an enhanced resistance to HU reminiscent ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl100
更新日期:2006-04-05 00:00:00
abstract::The synthesis of oligonucleotides (ODNs) containing 5-(N-aminohexyl)carbamoyl-2'-O-methyluridine (D) is described, and thermal stability and resistance to enzymatic hydrolysis of the ODNs are compared with ODNs containing 5-(N-aminohexyl)carbamoyl-2'-deoxyuridine (H). The ODNs containing D and the complementary RNA de...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg374
更新日期:2003-05-15 00:00:00
abstract::YB-1 is a universal major protein of cytoplasmic mRNPs, a member of the family of multifunctional cold shock domain proteins (CSD proteins). Depending on its amount on mRNA, YB-1 stimulates or inhibits mRNA translation. In this study, we have analyzed complexes formed in vitro at various YB-1 to mRNA ratios, including...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh889
更新日期:2004-10-19 00:00:00
abstract::The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional activity of, and the protein-DNA interactions that occur within, the proximal region of the human beta-actin promoter. Eff...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.24.6913
更新日期:1991-12-25 00:00:00
abstract::Mammalian cryptochromes (Crys) are essential circadian clock factors implicated in diverse clock-independent physiological functions, including DNA damage responses. Here we show that Cry1 modulates the ATR-mediated DNA damage checkpoint (DDC) response by interacting with Timeless (Tim) in a time-of-day-dependent mann...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku094
更新日期:2014-04-01 00:00:00
abstract::Heptadecaoligodeoxyribonucleotides containing one or more of the bases, 6H,8H-3,4-dihydropyrimido[4,5-c][1,2]oxazin-7-one (P), 2-amino-6-methoxyaminopurine (K), and hypoxanthine (I) and combinations of P with K and I have been synthesised on a DNA synthesiser. The stability of duplexes containing these basemodified ol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.19.5149
更新日期:1992-10-11 00:00:00
abstract::Allele-specific protospacer adjacent motif (asPAM)-positioning SNPs and CRISPRs are valuable resources for gene therapy of dominant disorders. However, one technical hurdle is to identify the haplotype comprising the disease-causing allele and the distal asPAM SNPs. Here, we describe a novel CRISPR-based method (CRISP...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1233
更新日期:2020-03-18 00:00:00
abstract::Bulky lesions in the template strand block the progression of RNA polymerase II (RNAP II) and are repaired more rapidly than lesions in the non-transcribed strand, which do not block transcription. In order to better understand the basis of this transcription-coupled repair we developed an in vitro system with purifie...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.4.787
更新日期:1997-02-15 00:00:00
abstract::Drosophila tissue culture cells have been important in the study of homologous promoters and more recently in the study of mammalian transcriptional factors such as CTF and SP1 which bind and stimulate transcription from transfected genes. In this paper we show that a Drosophila melanogaster haploid cell line (1182-4)...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.15.6205
更新日期:1989-08-11 00:00:00
abstract::Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by optical mapping of their DNA. We use a novel, one-step chemo-enzymatic reaction to covalently bind fluorophores to DNA at the four-base recognition sites of a ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv563
更新日期:2015-10-15 00:00:00
abstract::Communication between distantly spaced genomic regions is one of the key features of gene regulation in eukaryotes. Chromatin per se can stimulate efficient enhancer-promoter communication (EPC); however, the role of chromatin structure and dynamics in this process remains poorly understood. Here we show that nucleoso...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1240
更新日期:2017-04-07 00:00:00
abstract::We have analyzed the ATPase activity exhibited by the UvrABC DNA repair complex. The UvrA protein is an ATPase whose lack of DNA dependence may be related to the ATP induced monomer-dimer transitions. ATP induced dimerization may be responsible for the enhanced DNA binding activity observed in the presence of ATP. Alt...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.11.4145
更新日期:1989-06-12 00:00:00
abstract::Ribonucleic acids are highly conserved essential parts of cellular life. RNA function is determined to a large extent by its hydrodynamic behaviour. The presented study proposes a strategy to predict the hydrodynamic behaviour of RNA single strands on the basis of the polymer size. By atom-level shell-modelling of hig...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq808
更新日期:2011-02-01 00:00:00
abstract::A mass spectral method is described for the detection and identification of unusual nucleotide residues present in DNAs. Analysis by this method of intact, underivatized DNA from salmon sperm, calf thymus, mouse L-cells, wheat germ, M. lysodeikticus, E. Coli, and the bacteriophages 0X-174, fd, and lamda, yields diagno...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.11.2959
更新日期:1976-11-01 00:00:00
abstract::The precursor to the Euglena light harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) is a polyprotein containing multiple copies of LHCPII covalently joined by a decapeptide linker. cDNA and genomic clones encoding the 5' and 3' end of a 6.6 kb LHCPII mRNA were sequenced. A 3.1 kb genomic region en...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.25.5737
更新日期:1994-12-25 00:00:00
abstract::The long terminal repeat (LTR) of the proviral human immunodeficiency virus (HIV)-1 genome is integral to virus transcription and host cell infection. The guanine-rich U3 region within the LTR promoter, previously shown to form G-quadruplex structures, represents an attractive target to inhibit HIV transcription and r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw432
更新日期:2016-07-27 00:00:00
abstract::COMPASS is a method for homology detection and local alignment construction based on the comparison of multiple sequence alignments (MSAs). The method derives numerical profiles from given MSAs, constructs local profile-profile alignments and analytically estimates E-values for the detected similarities. Until now, CO...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm293
更新日期:2007-07-01 00:00:00
abstract::The stability of chromosome ends, the telomeres, is dependent on the ribonucleoprotein telomerase. In vitro, telomerase requires at least one RNA molecule and a reverse transcriptase-like protein. However, for telomere homeostasis in vivo, additional proteins are required. Telomerase RNAs of different species vary in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt514
更新日期:2013-09-01 00:00:00
abstract::Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks223
更新日期:2012-07-01 00:00:00
abstract::Messenger RNA in eukaryotes is comprised of several abundance classes. Also, the decay of these unstable molecules shows at least two very different lifetimes. Two independent techniques are used here to examine the relation between message abundance and lifetime in cell lines from very different organisms. The method...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.8.3057
更新日期:1978-08-01 00:00:00
abstract::RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku422
更新日期:2014-07-01 00:00:00
abstract::The unsymmetrical cyanine dye thiazole orange homodimer (TOTO) binds to single-stranded DNA (ssDNA, M13mp18 ssDNA) to form a fluorescent complex that is stable under the standard conditions of electrophoresis. The stability of this complex is indistinguishable from that of the corresponding complex of TOTO with double...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.7.1215
更新日期:1995-04-11 00:00:00
abstract::Retroviral particles contain a dimeric RNA genome, which serves as template for the generation of double-stranded DNA by reverse transcription. Transfer between RNA strands during DNA synthesis is governed by both sequence similarity between templates and structural features of the dimeric RNA. A kissing hairpin, beli...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh159
更新日期:2004-01-09 00:00:00
abstract::We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutationa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.12.2318
更新日期:1994-06-25 00:00:00
abstract::Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we used a library scree...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr609
更新日期:2011-10-01 00:00:00
abstract::The transactivator Staf, which contains seven contiguous zinc fingers of the C(2)-H(2)type, exerts its effects on gene expression by binding to specific targets in vertebrate small nuclear RNA (snRNA) and snRNA-type gene promoters. Here, we have investigated the interaction of the Staf zinc finger domain with the opti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.10.2114
更新日期:2000-05-15 00:00:00