BMI1 is a target gene of E2F-1 and is strongly expressed in primary neuroblastomas.


:The oncogene BMI1 encodes a polycomb group transcription factor that is required for embryonic development and self-renewal of stem cells. Despite these important functions little is known about the regulation of BMI1 expression. A cDNA microarray based search for target genes of E2F-1 in neuroblastoma cells expressing a 4-OHT-regulated E2F-1-ER fusion protein identified many hitherto unknown E2F-1 regulated genes. A total of 10% of these genes, including BMI1, encode proteins that function primarily in the regulation of gene expression. The BMI1 promoter contains a putative E2F binding site that was required for the activation of a BMI1 promoter-dependent reporter construct by E2F-1. Chromatin immunoprecipitation revealed 4-OHT-dependent binding of E2F-1-ER and binding of endogenous E2F-1 to the BMI1 promoter in tumor cells. We have previously shown activation of the oncogene MYCN by E2F. Thus, in neuroblastomas deregulated E2F-1 can activate two oncogenes, MYCN and BMI1 that are known to co-operate in tumor formation. Consistent with a role of Bmi1 in neuroblastoma tumorigenesis we found strong Bmi1 expression in primary neuroblastomas. Our results reveal a novel link between E2F and polycomb transcription factors and suggest a role of Bmi1 in neuroblastomas.


Nucleic Acids Res


Nucleic acids research


Nowak K,Kerl K,Fehr D,Kramps C,Gessner C,Killmer K,Samans B,Berwanger B,Christiansen H,Lutz W





Has Abstract


2006-03-31 00:00:00














  • Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes.

    abstract::Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and cr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Crespo M,Damont A,Blanco M,Lastrucci E,Kennani SE,Ialy-Radio C,Khattabi LE,Terrier S,Louwagie M,Kieffer-Jaquinod S,Hesse AM,Bruley C,Chantalat S,Govin J,Fenaille F,Battail C,Cocquet J,Pflieger D

    更新日期:2020-05-07 00:00:00

  • Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors.

    abstract::The U1 snRNP-specific 70K protein is one of the few snRNP proteins from higher eukaryotic cells that is phosphorylated in vivo (1,2). Immunoaffinity purified spliceosomal snRNPs (U1, U2, U5, and U4/U6) were tested for their ability to phosphorylate in vitro the U1-specific 70K protein. An snRNP-associated kinase activ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Woppmann A,Will CL,Kornstädt U,Zuo P,Manley JL,Lührmann R

    更新日期:1993-06-25 00:00:00

  • Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.

    abstract::The sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA has been determined. The sequence contains the entire coat protein cistron as well as regions to its left and right. Sequence characterization was by conventional methods for use with uniformly 32P labeled RNA complemented by newer methods for ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Guilley H,Jonard G,Kukla B,Richards KE

    更新日期:1979-04-01 00:00:00

  • Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.

    abstract::NONO, SFPQ and PSPC1 make up a family of proteins with diverse roles in transcription, RNA processing and DNA double-strand break (DSB) repair. To understand long-term effects of loss of NONO, we characterized murine embryonic fibroblasts (MEFs) from knockout mice. In the absence of genotoxic stress, wild-type and mut...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Li S,Li Z,Shu FJ,Xiong H,Phillips AC,Dynan WS

    更新日期:2014-09-01 00:00:00

  • Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).

    abstract::Use of the polymerase chain reaction (PCR) to amplify variable numbers of tandem repeat (VNTR) loci has become widely used in genetic typing. Unfortunately, preferential amplification of small allelic products relative to large allelic products may result in incorrect or ambiguous typing in a heterozygous sample. The ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Demers DB,Curry ET,Egholm M,Sozer AC

    更新日期:1995-08-11 00:00:00

  • Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5'-terminal regions of cap0-, cap1- and 5'ppp- mRNAs.

    abstract::Ribosomal recruitment of cellular mRNAs depends on binding of eIF4F to the mRNA's 5'-terminal 'cap'. The minimal 'cap0' consists of N7-methylguanosine linked to the first nucleotide via a 5'-5' triphosphate (ppp) bridge. Cap0 is further modified by 2'-O-methylation of the next two riboses, yielding 'cap1' (m7GpppNmN) ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kumar P,Sweeney TR,Skabkin MA,Skabkina OV,Hellen CU,Pestova TV

    更新日期:2014-03-01 00:00:00

  • Finding approximate gene clusters with Gecko 3.

    abstract::Gene-order-based comparison of multiple genomes provides signals for functional analysis of genes and the evolutionary process of genome organization. Gene clusters are regions of co-localized genes on genomes of different species. The rapid increase in sequenced genomes necessitates bioinformatics tools for finding g...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Winter S,Jahn K,Wehner S,Kuchenbecker L,Marz M,Stoye J,Böcker S

    更新日期:2016-11-16 00:00:00

  • Resolution of a Holliday junction by vaccinia topoisomerase requires a spacer DNA segment 3' of the CCCTT/ cleavage sites.

    abstract::Vaccinia virus DNA topoisomerase catalyzes resolution of synthetic Holliday junctions in vitro. The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/, that are opposed within a partially mobile four-way junction. Efficient resolution occurs on a junction with a 10 bp segment of branc...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sekiguchi J,Cheng C,Shuman S

    更新日期:2000-07-15 00:00:00

  • Determination of the molecular weight of DNA-bound protein(s) responsible for gel electrophoretic mobility shift of linear DNA fragments examplified with purified viral myb protein.

    abstract::A protein-DNA complex has less gel electrophoretic mobility than the free DNA fragment. One parameter for the degree of retardation of a linear DNA fragment in a protein-DNA complex is the molecular weight of the bound protein(s). The quotient of the migration distances of free DNA (m) and protein-DNA complex (m') is ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bading H

    更新日期:1988-06-24 00:00:00

  • Identification and analysis of the Arabidopsis thaliana BSH gene, a member of the SNF5 gene family.

    abstract::The multiprotein complexes involved in active dis-ruption of chromatin structure, homologous to yeast SWI/SNF complex, have been described for human and Drosophila cells. In all SWI/SNF-class complexes characterised so far, one of the key components is the SNF5-type protein. Here we describe the isolation of a plant (...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Brzeski J,Podstolski W,Olczak K,Jerzmanowski A

    更新日期:1999-06-01 00:00:00

  • Local conformational variations observed in B-DNA crystals do not improve base stacking: computational analysis of base stacking in a d(CATGGGCCCATG)(2) BA intermediate crystal structure.

    abstract::The crystal structure of d(CATGGGCCCATG)(2) shows unique stacking patterns of a stable B<-->A-DNA intermediate. We evaluated intrinsic base stacking energies in this crystal structure using an ab initio quantum mechanical method. We found that all crystal base pair steps have stacking energies close to their values in...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Poner J,Florián J,Ng HL,Poner JE,Packová N

    更新日期:2000-12-15 00:00:00

  • Alternative splicing at GYNNGY 5' splice sites: more noise, less regulation.

    abstract::Numerous eukaryotic genes are alternatively spliced. Recently, deep transcriptome sequencing has skyrocketed proportion of alternatively spliced genes; over 95% human multi-exon genes are alternatively spliced. One fundamental question is: are all these alternative splicing (AS) events functional? To look into this is...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wang M,Zhang P,Shu Y,Yuan F,Zhang Y,Zhou Y,Jiang M,Zhu Y,Hu L,Kong X,Zhang Z

    更新日期:2014-12-16 00:00:00

  • Designating eukaryotic orthology via processed transcription units.

    abstract::Orthology is a widely used concept in comparative and evolutionary genomics. In addition to prokaryotic orthology, delineating eukaryotic orthology has provided insight into the evolution of higher organisms. Indeed, many eukaryotic ortholog databases have been established for this purpose. However, unlike prokaryotes...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ho MR,Jang WJ,Chen CH,Ch'ang LY,Lin WC

    更新日期:2008-06-01 00:00:00

  • Hydration of single-stranded phosphodiester and phosphorothioate oligodeoxyribonucleotides.

    abstract::Infrared spectroscopy was used to identify hydration-sensitive structural differences between single- stranded phosphorothioate (PS) and phosphodiester (PO) oligodeoxyribonucleotides. Spectra were recorded in the mid-infrared region, 500-1800 cm-1, at relative humidities between 0 and 98%; the PS and PO spectra are su...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: White AP,Reeves KK,Snyder E,Farrell J,Powell JW,Mohan V,Griffey RH

    更新日期:1996-08-15 00:00:00

  • Quantitation of RNA editing substrates, products and potential intermediates: implications for developmental regulation.

    abstract::Kinetoplast mitochondrial RNA editing is the developmentally regulated post-transcriptional process of uridine insertion and deletion in mRNAs directed by short guide RNAs (gRNAs), which creates functional mRNAs. Two mechanisms are proposed: transesterification which predicts gRNA/mRNA chimeric intermediates, and enzy...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Riley GR,Myler PJ,Stuart K

    更新日期:1995-02-25 00:00:00

  • Pyrophosphate-condensing activity linked to nucleic acid synthesis.

    abstract::In some preparations of DNA dependent RNA polymerase a new enzymatic activity has been found which catalyzes the condensation of two pyrophosphate molecules, liberated in the process of RNA synthesis, to one molecule of orthophosphate and one molecule of Mg (or Mn) - chelate complex with trimetaphosphate. This activit...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Volloch VZ,Rits S,Tumerman L

    更新日期:1979-04-01 00:00:00

  • 2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis.

    abstract::The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2-10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hardwick JS,Haugland MM,El-Sagheer AH,Ptchelkine D,Beierlein FR,Lane AN,Brown T,Lovett JE,Anderson EA

    更新日期:2020-04-06 00:00:00

  • Qgrid: clustering tool for detecting charged and hydrophobic regions in proteins.

    abstract::We have developed a simple but powerful method and web server to quickly locate charged and hydrophobic clusters in proteins ( For the charged clusters, each atom in the protein is first assigned a charge according to a standard force field. Then a box is created with dimensions...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ahmad S,Sarai A

    更新日期:2004-07-01 00:00:00

  • Three new members of the RNP protein family in Xenopus.

    abstract::Many RNP proteins contain one or more copies of the RNA recognition motif (RRM) and are thought to be involved in cellular RNA metabolism. We have previously characterized in Xenopus a nervous system specific gene, nrp1, that is more similar to the hnRNP A/B proteins than to other known proteins (K. Richter, P. J. Goo...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Good PJ,Rebbert ML,Dawid IB

    更新日期:1993-02-25 00:00:00

  • Binding of the EcoRII methyltransferase to 5-fluorocytosine-containing DNA. Isolation of a bound peptide.

    abstract::The properties of the interaction of 5-fluorocytosine-containing DNA with the EcoRII methyltransferase were studied. The DNA used was either a polymer synthesized in vitro, or a 20-mer containing one CCA/TGG sequence. The DNA could be methylated by the enzyme. In the process the enzyme formed a tight binding adduct wi...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Friedman S,Ansari N

    更新日期:1992-06-25 00:00:00

  • Electron microscopy of the reactions of anti-poly A. poly U and anti-poly I. poly C antibodies with synthetic polynucleotide complexes and natural nucleic acids.

    abstract::The reactions between purified anti-poly A. poly U and-poly I. poly C. antibodies (IgG and IgM), and synthetic and natural polynucleotides were visualized at the molecular level. This was achieved by the use of fine tungsten bidirectional shadowing of molecules adsorbed onto thin carbon films, combined with dark field...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Nahon-Merlin E,Delain E,Coulaud D,Lacour F

    更新日期:1980-04-25 00:00:00

  • Molecular mechanism of upregulation of survivin transcription by the AT-rich DNA-binding ligand, Hoechst33342: evidence for survivin involvement in drug resistance.

    abstract::We have previously shown that hedamycin, a GC-rich DNA-binding antitumor agent, downregulates survivin transcription (Wu et al. (2005) Molecular mechanism of inhibition of survivin transcription by the GC-rich sequence selective DNA-binding antitumor agent, hedamycin: evidence of survivin downregulation associated wit...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wu J,Apontes P,Song L,Liang P,Yang L,Li F

    更新日期:2007-01-01 00:00:00

  • Detection of oligonucleotide hybridization at femtomolar level and sequence-specific gene analysis of the Arabidopsis thaliana leaf extract with an ultrasensitive surface plasmon resonance spectrometer.

    abstract::A flow-injection (FI) device is combined, through the use of a low-volume (4 microl) flow cell, with an ultrasensitive surface plasmon resonance (SPR) spectrometer equipped with a bi-cell photodiode detector. The application of this novel FI-SPR device for sequence-specific ultratrace analysis of oligodeoxynucleotides...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Song F,Zhou F,Wang J,Tao N,Lin J,Vellanoweth RL,Morquecho Y,Wheeler-Laidman J

    更新日期:2002-07-15 00:00:00

  • Transcriptional and post-transcriptional regulation of storage protein gene expression in sulfur-deficient pea seeds.

    abstract::We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S defic...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Beach LR,Spencer D,Randall PJ,Higgins TJ

    更新日期:1985-02-11 00:00:00

  • Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer: insight into RNA-binding properties of bacterial Hfq.

    abstract::Bacterial Hfq is a protein that plays an important role in the regulation of genes in cooperation with sRNAs. Escherichia coli Hfq (EcHfq) has two or more sites that bind RNA(s) including U-rich and/or the poly(A) tail of mRNA. However, functional and structural information about Bacillus subtilis Hfq (BsHfq) includin...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Someya T,Baba S,Fujimoto M,Kawai G,Kumasaka T,Nakamura K

    更新日期:2012-02-01 00:00:00

  • Esc2 promotes telomere stability in response to DNA replication stress.

    abstract::Telomeric regions of the genome are inherently difficult-to-replicate due to their propensity to generate DNA secondary structures and form nucleoprotein complexes that can impede DNA replication fork progression. Precisely how cells respond to DNA replication stalling within a telomere remains poorly characterized, l...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jørgensen SW,Liberti SE,Larsen NB,Lisby M,Mankouri HW,Hickson ID

    更新日期:2019-05-21 00:00:00

  • REBASE - restriction enzymes and methylases.

    abstract::REBASE is a comprehensive database of information about restriction enzymes and their associated methylases, including their recognition and cleavage sites and their commercial availability. Also included is a listing of homing endonucleases. Information from REBASE is available via monthly electronic mailings as well...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Roberts RJ,Macelis D

    更新日期:1998-01-01 00:00:00

  • Sequential stimulation of cellular RNA synthesis in polyoma-infected mouse kidney cell cultures.

    abstract::Lytic infection with polyoma virus leads in Go-arrested primary mouse kidney cell cultures to a mitotic host response. In the present work we focused our attention on cellular RNA synthesis shortly after onset of polyoma T-antigen synthesis. Onset of polyoma-induced stimulation of 45S pre-rRNA synthesis was determined...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Matter JM,Tiercy JM,Weil R

    更新日期:1983-10-11 00:00:00

  • PlantLoc: an accurate web server for predicting plant protein subcellular localization by substantiality motif.

    abstract::Knowledge of subcellular localizations (SCLs) of plant proteins relates to their functions and aids in understanding the regulation of biological processes at the cellular level. We present PlantLoc, a highly accurate and fast webserver for predicting the multi-label SCLs of plant proteins. The PlantLoc server has two...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tang S,Li T,Cong P,Xiong W,Wang Z,Sun J

    更新日期:2013-07-01 00:00:00

  • Curved DNA fragments display retarded elution upon anion exchange HPLC.

    abstract::Restriction fragments containing established curved regions, such as the pBR322 tn3 region, the phage lambda attP region and the SV40 T-antigen and terminus of replication regions, exhibit systematically retarded elution upon anion exchange based HPLC. Using this method, we were able to detect readily other SV40 curve...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Fack F,Sarantoglou V

    更新日期:1991-08-11 00:00:00