Nucleotide sequences of three tRNA genes encoded in Tetrahymena mitochondrial DNA.

abstract：:Linkage analysis is a successful procedure to associate diseases with specific genomic regions. These regions are often large, containing hundreds of genes, which make experimental methods employed to identify the disease gene arduous and expensive. We present two methods to prioritize candidates for further experimen...

journal_title：Nucleic acids research

authors： George RA,Liu JY,Feng LL,Bryson-Richardson RJ,Fatkin D,Wouters MA

更新日期：2006-01-01 00:00:00

abstract：:The secondary structure of an adenovirus associated low molecular weight RNA (VAI-RNA) has been studied by partial digestion with T1-RNase and S1-endonuclease followed by T1-fingerprint analysis. The empirical secondary structure has been compared with two computer generated models based on minimal free energy of the ...

journal_title：Nucleic acids research

authors： Monstein HJ,Philipson L

更新日期：1981-09-11 00:00:00

abstract：:Actinomycin D (ActD) is a small molecule with strong antibiotic and anticancer activity. However, its biologically relevant DNA-binding mechanism has never been resolved, with some studies suggesting that the primary binding mode is intercalation, and others suggesting that single-stranded DNA binding is most importan...

journal_title：Nucleic acids research

authors： Paramanathan T,Vladescu I,McCauley MJ,Rouzina I,Williams MC

更新日期：2012-06-01 00:00:00

abstract：:According to the recruitment model of transcriptional activation, an activator helps initiate transcription by bringing the RNA polymerase to a specific location on the DNA through interaction with components of the transcriptional machinery. However, it is difficult to isolate and define the activities of specific ac...

journal_title：Nucleic acids research

authors： Wang S,Shepard JR,Shi H

更新日期：2010-04-01 00:00:00

abstract：:Effective drug discovery and optimization can be accelerated by techniques capable of deconvoluting the complexities often present in targeted biological systems. We report a single-molecule approach to study the binding of an alternative splicing regulator, muscleblind-like 1 protein (MBNL1), to (CUG)n = 4,6 and the ...

journal_title：Nucleic acids research

authors： Haghighat Jahromi A,Honda M,Zimmerman SC,Spies M

更新日期：2013-07-01 00:00:00

abstract：:HIV-1 Tat protein reprograms cellular gene expression of infected as well as uninfected cells apart from its primary function of transactivating HIV-1 long terminal repeat (LTR) promoter by binding to a nascent RNA stem-loop structure known as the transactivator response region (TAR). Tat also induces chromatin remode...

journal_title：Nucleic acids research

authors： Dandekar DH,Ganesh KN,Mitra D

更新日期：2004-02-23 00:00:00

abstract：:Abasic (AP) sites, the most common DNA lesions are frequently associated with double strand breaks (DSBs) and can pose a block to the final ligation. In many prokaryotes, nonhomologous end joining (NHEJ) repair of DSBs relies on a two-component machinery constituted by the ring-shaped DNA-binding Ku that recruits the ...

journal_title：Nucleic acids research

authors： de Ory A,Carabaña C,de Vega M

更新日期：2019-06-04 00:00:00

abstract：:In order to examine the nature of the complex formation between the ribosomal protein S1 and nucleic acids three methods were used: Inhibition of the reaction of n-ethyl[2.3 14C]-maleimide with S1 by the addition of oligonucleotides; adsorption of the complexes to nitrocellulose filters; and equilibrium dialysis. The ...

journal_title：Nucleic acids research

authors： Lipecky R,Kohlschein J,Gassen HG

更新日期：1977-10-01 00:00:00

abstract：:The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were u...

journal_title：Nucleic acids research

authors： Feigon J,Wang AH,van der Marel GA,Van Boom JH,Rich A

更新日期：1984-01-25 00:00:00

abstract：:The mini-exon, a short segment found at the 5' end of trypanosome mRNAs, is contributed by a small RNA, the mini-exon donor (medRNA). In vivo 32P-labeled medRNA, a set of smaller RNAs related to it, and mRNA, were purified from Trypanosoma brucei by hybrid selection and gel electrophoresis. Using RNA fingerprinting an...

journal_title：Nucleic acids research

authors： Freistadt MS,Cross GA,Branch AD,Robertson HD

更新日期：1987-12-10 00:00:00

abstract：:Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel ...

journal_title：Nucleic acids research

authors： Shinnick TM,Lund E,Smithies O,Blattner FR

更新日期：1975-10-01 00:00:00

abstract：:The ability to integrate 'omics' (i.e. transcriptomics and proteomics) is becoming increasingly important to the understanding of regulatory mechanisms. There are currently no tools available to identify differentially expressed genes (DEGs) across different 'omics' data types or multi-dimensional data including time ...

journal_title：Nucleic acids research

authors： Tang S,Hemberg M,Cansizoglu E,Belin S,Kosik K,Kreiman G,Steen H,Steen J

更新日期：2016-06-02 00:00:00

abstract：:Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene expression profiling. In the first step, multiple primer pairs are added to the RNA to be analysed together with reverse transcriptase and Taq DNA polymerase. Following reverse transcription, the multiplexed amplicons are simultaneously amplified...

journal_title：Nucleic acids research

authors： Stanley KK,Szewczuk E

更新日期：2005-11-24 00:00:00

abstract：:We have recently developed a novel method for the affinity purification of the complete suite of translating mRNA from genetically labeled cell populations. This method permits comprehensive quantitative comparisons of the genes employed by each specific cell type. We provide a detailed description of tools for analys...

journal_title：Nucleic acids research

authors： Dougherty JD,Schmidt EF,Nakajima M,Heintz N

更新日期：2010-07-01 00:00:00

abstract：:Fluorescence-based sequencing is playing an increasingly important role in efforts to identify DNA polymorphisms and mutations of biological and medical interest. The application of this technology in generating the reference sequence of simple and complex genomes is also driving the development of new computer progra...

journal_title：Nucleic acids research

authors： Nickerson DA,Tobe VO,Taylor SL

更新日期：1997-07-15 00:00:00

abstract：:IHF and HU are two heterodimeric nucleoid-associated proteins (NAP) that belong to the same protein family but interact differently with the DNA. IHF is a sequence-specific DNA-binding protein that bends the DNA by over 160°. HU is the most conserved NAP, which binds non-specifically to duplex DNA with a particular pr...

journal_title：Nucleic acids research

authors： Prieto AI,Kahramanoglou C,Ali RM,Fraser GM,Seshasayee AS,Luscombe NM

更新日期：2012-04-01 00:00:00

abstract：:The chromatin structure of yeast ribosomal DNA was analyzed in vivo by crosslinking intact cells with psoralen. We found that in exponentially growing cultures the regions coding for the 35S rRNA precursor fall into two distinct classes. One class was highly accessible to psoralen and associated with nascent RNAs, cha...

journal_title：Nucleic acids research

authors： Dammann R,Lucchini R,Koller T,Sogo JM

更新日期：1993-05-25 00:00:00

abstract：:Genes from yeast to mammals are frequently subject to non-coding transcription of their antisense strand; however the genome-wide role for antisense transcription remains elusive. As transcription influences chromatin structure, we took a genome-wide approach to assess which chromatin features are associated with nasc...

journal_title：Nucleic acids research

authors： Murray SC,Haenni S,Howe FS,Fischl H,Chocian K,Nair A,Mellor J

更新日期：2015-09-18 00:00:00

abstract：:Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Here we report the interesting phenomenon that puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length ...

journal_title：Nucleic acids research

authors： Miyamoto-Sato E,Nemoto N,Kobayashi K,Yanagawa H

更新日期：2000-03-01 00:00:00

abstract：:Five peaks of DNA glycosylase activity showing a preference for MNNG alkylated DNA have been identified from extracts of adapted M. luteus. They are numerically designated as GI to GV in order of their decreasing molecular weights. The first two of these peaks have been highly purified. GI, is a constitutive heat labi...

journal_title：Nucleic acids research

authors： Riazuddin S,Athar A,Ahmed Z,Lali SM,Sohail A

更新日期：1987-08-25 00:00:00

abstract：:Escherichia coli Exonuclease I (ExoI) digests single-stranded DNA (ssDNA) in the 3'-5' direction in a highly processive manner. The crystal structure of ExoI, determined previously in the absence of DNA, revealed a C-shaped molecule with three domains that form a central positively charged groove. The active site is a...

journal_title：Nucleic acids research

authors： Korada SK,Johns TD,Smith CE,Jones ND,McCabe KA,Bell CE

更新日期：2013-06-01 00:00:00

abstract：:In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...

journal_title：Nucleic acids research

authors： Stefanovic B,Hackl W,Lührmann R,Schümperli D

更新日期：1995-08-25 00:00:00

abstract：:The RIKEN Arabidopsis Genome Encyclopedia (RARGE) database houses information on biological resources ranging from transcriptome to phenome, including RIKEN Arabidopsis full-length (RAFL) complementary DNAs (cDNAs), their promoter regions, Dissociation (Ds) transposon-tagged lines and expression data from microarray e...

journal_title：Nucleic acids research

authors： Sakurai T,Satou M,Akiyama K,Iida K,Seki M,Kuromori T,Ito T,Konagaya A,Toyoda T,Shinozaki K

更新日期：2005-01-01 00:00:00

abstract：:Although mobility of the phylogenetically widespread group I introns appears to be mechanistically similar, the phage T4 intron-encoded endonucleases that promote mobility of the td and sunY introns are different from their eukaryotic counterparts. Most notably, they cleave at a distance from the intron insertion site...

journal_title：Nucleic acids research

authors： Bell-Pedersen D,Quirk S,Clyman J,Belfort M

更新日期：1990-07-11 00:00:00

abstract：:The MED1/TRAP220 subunit of the Mediator plays a key role in facilitating ligand-dependent interactions of this multisubunit coactivator complex with nuclear receptors through their ligand binding domains. The isolated MED1/TRAP220 protein previously was shown to interact with glucocorticoid receptor (GR) in a ligand-...

journal_title：Nucleic acids research

authors： Chen W,Roeder RG

更新日期：2007-01-01 00:00:00

abstract：:We show that in mouse myoblasts the MyoD1 promoter is highly stimulated by MyoD1 expression, suggesting that it is controlled by a positive feedback loop. Using deletion and mutation analyses, we identified the targets for MyoD1 promoter autoregulation as the two proximal E-boxes located close to the MyoD1 core promot...

journal_title：Nucleic acids research

authors： Zingg JM,Pedraza-Alva G,Jost JP

更新日期：1994-06-25 00:00:00

abstract：:Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...

journal_title：Nucleic acids research

authors： Pavlov NA,Cherny DI,Nazimov IV,Slesarev AI,Subramaniam V

更新日期：2002-02-01 00:00:00

abstract：:Synthetic genetic sensors and circuits enable programmable control over timing and conditions of gene expression and, as a result, are increasingly incorporated into the control of complex and multi-gene pathways. Size and complexity of genetic circuits are growing, but stay limited by a shortage of regulatory parts t...

journal_title：Nucleic acids research

authors： Bervoets I,Van Brempt M,Van Nerom K,Van Hove B,Maertens J,De Mey M,Charlier D

更新日期：2018-02-28 00:00:00

abstract：:A 200 bp sequence including a stretch of 54 base pairs of alternating guanosine and adenosine nucleotide residues [(dG-dA)27.(dT-dC)27] was cloned in the simian virus 40 (SV40) genome between the KpnI and HpaII sites. This sequence was discovered earlier as part of a region limiting the amplification of sequences adja...

journal_title：Nucleic acids research

authors： Rao BS,Manor H,Martin RG

更新日期：1988-08-25 00:00:00

abstract：:In vitro enzymatic amplification of nucleic acids by PCR or other techniques is a very sensitive method to detect rare DNA segments. We present here a protocol that allows the rapid, sensitive and precise quantification of DNA molecules using PCR amplification run to saturation. The DNA (or cDNA) to be assayed is co-a...

journal_title：Nucleic acids research

authors： Pannetier C,Delassus S,Darche S,Saucier C,Kourilsky P

更新日期：1993-02-11 00:00:00