Abstract：
:Fluorescence-based sequencing is playing an increasingly important role in efforts to identify DNA polymorphisms and mutations of biological and medical interest. The application of this technology in generating the reference sequence of simple and complex genomes is also driving the development of new computer programs to automate base calling (Phred), sequence assembly (Phrap) and sequence assembly editing (Consed) in high throughput settings. In this report we describe a new computer program known as PolyPhred that automatically detects the presence of heterozygous single nucleotide substitutions by fluorescencebased sequencing of PCR products. Its operations are integrated with the use of the Phred, Phrap and Consed programs and together these tools generate a high throughput system for detecting DNA polymorphisms and mutations by large scale fluorescence-based resequencing. Analysis of sequences containing known DNA variants demonstrates that the accuracy of PolyPhred with single pass data is >99% when the sequences are generated with fluorescent dye-labeled primers and approximately 90% for those prepared with dye-labeled terminators.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Nickerson DA,Tobe VO,Taylor SLdoi
10.1093/nar/25.14.2745subject
Has Abstractpub_date
1997-07-15 00:00:00pages
2745-51issue
14eissn
0305-1048issn
1362-4962pii
gka450journal_volume
25pub_type
杂志文章abstract：:Evidence is presented that the editing mechanisms of aminoacyl-tRNA synthetase operate by two alternative pathways: pre-transfer, by hydrolysis of the non-cognate aminoacyl adenylate; post-transfer, by hydrolysis of the mischarged tRNA. The methionyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophil...
journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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pub_type： 杂志文章
doi：10.1093/nar/25.8.1493
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1136
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.9.3425
更新日期：1989-05-11 00:00:00
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pub_type： 杂志文章
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更新日期：1992-12-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.6.1439
更新日期：2000-03-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：2008-02-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2009-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz949
更新日期：2020-01-08 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2018-04-20 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.5.1863
更新日期：1979-01-01 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/gkt257
更新日期：2013-06-01 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/28.10.2187
更新日期：2000-05-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：2001-10-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.18.4537
更新日期：1981-09-25 00:00:00
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journal_title：Nucleic acids research
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更新日期：1978-09-01 00:00:00
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更新日期：2011-12-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2018-07-02 00:00:00
abstract：:The abundance and cell cycle dependent expression of the mRNA for human nonhistone protein HMG-17 were studied in synchronized HeLa cells. Slot blot analysis indicates that the HMG-17 mRNA is a very abundant message, significantly more so than histone or actin mRNA. RNA prepared from tissue culture cells contains high...
journal_title：Nucleic acids research
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更新日期：1987-04-24 00:00:00
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更新日期：2011-01-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：1988-12-23 00:00:00
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更新日期：2003-07-15 00:00:00
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更新日期：2007-01-01 00:00:00
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更新日期：2008-12-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1235
更新日期：2013-01-01 00:00:00
