The role of GTP in transient splitting of 70S ribosomes by RRF (ribosome recycling factor) and EF-G (elongation factor G).

abstract：:The Ensembl project (https://www.ensembl.org) annotates genomes and disseminates genomic data for vertebrate species. We create detailed and comprehensive annotation of gene structures, regulatory elements and variants, and enable comparative genomics by inferring the evolutionary history of genes and genomes. Our int...

journal_title：Nucleic acids research

authors： Howe KL,Achuthan P,Allen J,Allen J,Alvarez-Jarreta J,Amode MR,Armean IM,Azov AG,Bennett R,Bhai J,Billis K,Boddu S,Charkhchi M,Cummins C,Da Rin Fioretto L,Davidson C,Dodiya K,El Houdaigui B,Fatima R,Gall A,Garcia G

更新日期：2021-01-08 00:00:00

abstract：:Novel techniques for high-throughput steady-state metabolomic profiling yield information about changes of nearly thousands of metabolites. Such metabolomic profiles, when analyzed together with transcriptional profiles, can reveal novel insights about underlying biological processes. While a number of conceptual appr...

journal_title：Nucleic acids research

authors： Sergushichev AA,Loboda AA,Jha AK,Vincent EE,Driggers EM,Jones RG,Pearce EJ,Artyomov MN

更新日期：2016-07-08 00:00:00

abstract：:The Biodegradative Strain Database (BSD) is a freely-accessible, web-based database providing detailed information on degradative bacteria and the hazardous substances that they degrade, including corresponding literature citations, relevant patents and links to additional web-based biological and chemical data. The B...

journal_title：Nucleic acids research

authors： Urbance JW,Cole J,Saxman P,Tiedje JM

更新日期：2003-01-01 00:00:00

abstract：:The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chromophore. First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 nm are presented. The main Raman hands are assigned to the convenient nucleic bases by comparison with the sp...

journal_title：Nucleic acids research

authors： Chinsky L,Turpin PY

更新日期：1978-08-01 00:00:00

abstract：:ATP-dependent type I topoisomerases from extremely thermophilic archaebacteria--reverse gyrases--drive positive supercoiling of DNA. We showed that reverse gyrase from Desulfurococcus amylolyticus breaks the DNA at specific sites and covalently binds to the 5' end. In 30 out of 31 sites located in pBR322 DNA fragments...

journal_title：Nucleic acids research

authors： Kovalsky OI,Kozyavkin SA,Slesarev AI

更新日期：1990-05-11 00:00:00

abstract：:DNA synthesis by phage T4 DNA polymerase is arrested at specific sequences in single-stranded DNA templates. To determine whether or not T4 DNA polymerase accessory proteins 32, 44, 45 and 62 eliminated recognition of these arrest sites, unique primer-templates were constructed in which DNA synthesis began at a DNA pr...

journal_title：Nucleic acids research

authors： Charette MF,Weaver DT,DePamphilis ML

更新日期：1986-04-25 00:00:00

abstract：:In most eukaryotes, genetic exchange between paired homologs occurs in the context of a tripartite proteinaceous structure called the synaptonemal complex (SC). Genetic analyses have revealed that the genes encoding SC proteins are vital for meiotic chromosome pairing and recombination. However, the number, nature and...

journal_title：Nucleic acids research

authors： Anuradha S,Muniyappa K

更新日期：2004-04-28 00:00:00

abstract：:An endo-exonuclease has been purified from cultured monkey (CV-1) cells. The enzyme which was purified to near homogeneity to be a 65 kDa monomeric protein. The single-strand DNase activity is endonucleolytic and nonprocessive, whereas the double-strand DNase activity is exonucleolytic and processive. The enzyme was a...

journal_title：Nucleic acids research

authors： Couture C,Chow TY

更新日期：1992-08-25 00:00:00

abstract：:In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dual prokaryotic and e...

journal_title：Nucleic acids research

authors： Aoyama M,Agari K,Sun-Wada GH,Futai M,Wada Y

更新日期：2005-03-22 00:00:00

abstract：:ADAR enzymes, adenosine deaminases that act on RNA, form a family of RNA editing enzymes that convert adenosine to inosine within RNA that is completely or largely double-stranded. Site-selective A-->I editing has been detected at specific sites within a few structured pre-mRNAs of metazoans. We have analyzed the edit...

journal_title：Nucleic acids research

authors： Källman AM,Sahlin M,Ohman M

更新日期：2003-08-15 00:00:00

abstract：:Explicit solvent molecular dynamics (MD) was used to describe the intrinsic flexibility of the helix 42-44 portion of the 23S rRNA (abbreviated as Kt-42+rGAC; kink-turn 42 and GTPase-associated center rRNA). The bottom part of this molecule consists of alternating rigid and flexible segments. The first flexible segmen...

journal_title：Nucleic acids research

authors： Rázga F,Koca J,Mokdad A,Sponer J

更新日期：2007-01-01 00:00:00

abstract：:The MF alpha 1 gene of Saccharomyces cerevisiae is an alpha specific gene whose expression is regulated by two upstream activation sites (UASMF alpha 1S), which are the binding sites for activator proteins, MAT alpha 1 and MCM1. UASMF alpha 1 elements act in a synergistic fashion; lack of either element causes reduced...

journal_title：Nucleic acids research

authors： Inokuchi K,Nakayama A

更新日期：1991-06-11 00:00:00

abstract：:Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression tha...

journal_title：Nucleic acids research

authors： Kubo M,Nishiyama T,Tamada Y,Sano R,Ishikawa M,Murata T,Imai A,Lang D,Demura T,Reski R,Hasebe M

更新日期：2019-05-21 00:00:00

abstract：:MicroRNAs (miRNAs) can group together along the human genome to form stable secondary structures made of several hairpins hosting miRNAs in their stems. The few known examples of such structures are all involved in cancer development. A large scale computational analysis of human chromosomes crossing sequence analysis...

journal_title：Nucleic acids research

authors： Mathelier A,Carbone A

更新日期：2013-04-01 00:00:00

abstract：:We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing ...

journal_title：Nucleic acids research

authors： Pfoh R,Laatsch H,Sheldrick GM

更新日期：2008-06-01 00:00:00

abstract：:Ribonuclease MRP is an endonuclease, related to RNase P, which functions in eukaryotic pre-rRNA processing. In Saccharomyces cerevisiae, RNase MRP comprises an RNA subunit and ten proteins. To improve our understanding of subunit roles and enzyme architecture, we have examined protein-protein and protein-RNA interacti...

journal_title：Nucleic acids research

authors： Aspinall TV,Gordon JM,Bennett HJ,Karahalios P,Bukowski JP,Walker SC,Engelke DR,Avis JM

更新日期：2007-01-01 00:00:00

abstract：:Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and...

journal_title：Nucleic acids research

authors： Yue M,Ogawa Y

更新日期：2018-03-16 00:00:00

abstract：:A complete physical map of the spinach mitochondrial genome has been established. The entire sequence content of 327 kilobase pairs (kb) is postulated to occur as a single circular molecule. Two directly repeated elements of approximately 6 kb, located on this "master chromosome", are proposed to participate in an int...

journal_title：Nucleic acids research

authors： Stern DB,Palmer JD

更新日期：1986-07-25 00:00:00

abstract：:Due to the importance of protein phosphorylation in cellular control, many researches are undertaken to predict the kinase-specific phosphorylation sites. Referred to our previous work, KinasePhos 1.0, incorporated profile hidden Markov model (HMM) with flanking residues of the kinase-specific phosphorylation sites. H...

journal_title：Nucleic acids research

authors： Wong YH,Lee TY,Liang HK,Huang CM,Wang TY,Yang YH,Chu CH,Huang HD,Ko MT,Hwang JK

更新日期：2007-07-01 00:00:00

abstract：:As a trial practical application, we have applied optimized microfabricated electrophoresis devices, combined with enzymatic mutation detection methods, to the determination of single nucleotide polymorphism (SNP) sites in the p53 suppressor gene. Using clinical samples, we have achieved robust assays with quality fac...

journal_title：Nucleic acids research

authors： Schmalzing D,Belenky A,Novotny MA,Koutny L,Salas-Solano O,El-Difrawy S,Adourian A,Matsudaira P,Ehrlich D

更新日期：2000-05-01 00:00:00

abstract：:Using peptide arrays and binding to native histone proteins, we show that the ADD domain of Dnmt3a specifically interacts with the H3 histone 1-19 tail. Binding is disrupted by di- and trimethylation of K4, phosphorylation of T3, S10 or T11 and acetylation of K4. We did not observe binding to the H4 1-19 tail. The ADD...

journal_title：Nucleic acids research

authors： Zhang Y,Jurkowska R,Soeroes S,Rajavelu A,Dhayalan A,Bock I,Rathert P,Brandt O,Reinhardt R,Fischle W,Jeltsch A

更新日期：2010-07-01 00:00:00

abstract：:Unoxidized crystalline silicon, characterized by high purity, high homogeneity, sturdiness and an atomically flat surface, offers many advantages for the construction of electronic miniaturized biosensor arrays upon attachment of biomolecules (DNA, proteins or small organic compounds). This allows to study the inciden...

journal_title：Nucleic acids research

authors： Cattaruzza F,Cricenti A,Flamini A,Girasole M,Longo G,Prosperi T,Andreano G,Cellai L,Chirivino E

更新日期：2006-02-28 00:00:00

abstract：:We have sequenced the external transcribed spacer (ETS) of a ribosomal transcription unit from Xenopus laevis, together with sections of the preceding non-transcribed spacer. Our analysis was carried out on the same cloned transcription unit as that from which the internal transcribed spacers (ITS) were previously seq...

journal_title：Nucleic acids research

authors： Maden BE,Moss M,Salim M

更新日期：1982-04-10 00:00:00

abstract：:The frequencies of each of the 257 468 complete protein coding sequences (CDSs) have been compiled from the taxonomical divisions of the GenBank DNA sequence database. The sum of the codons used by 8792 organisms has also been calculated. The data files can be obtained from the anonymous ftp sites of DDBJ, Kazusa and ...

journal_title：Nucleic acids research

authors： Nakamura Y,Gojobori T,Ikemura T

更新日期：2000-01-01 00:00:00

abstract：:Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, w...

journal_title：Nucleic acids research

authors： Lucas I,Hyrien O

更新日期：2000-05-15 00:00:00

abstract：:Many microRNAs (miRNAs) are co-regulated during the same physiological process but the underlying cellular logic is often little understood. The conserved, immunomodulatory miRNAs miR-146 and miR-155, for instance, are co-induced in many cell types in response to microbial lipopolysaccharide (LPS) to feedback-repress ...

journal_title：Nucleic acids research

authors： Schulte LN,Westermann AJ,Vogel J

更新日期：2013-01-07 00:00:00

abstract：:Metagenomics is a relatively recently established but rapidly expanding field that uses high-throughput next-generation sequencing technologies to characterize the microbial communities inhabiting different ecosystems (including oceans, lakes, soil, tundra, plants and body sites). Metagenomics brings with it a number ...

journal_title：Nucleic acids research

authors： Hunter S,Corbett M,Denise H,Fraser M,Gonzalez-Beltran A,Hunter C,Jones P,Leinonen R,McAnulla C,Maguire E,Maslen J,Mitchell A,Nuka G,Oisel A,Pesseat S,Radhakrishnan R,Rocca-Serra P,Scheremetjew M,Sterk P,Vaughan D,

更新日期：2014-01-01 00:00:00

abstract：:The RNA degradosome is a multi-enzyme assembly that contributes to key processes of RNA metabolism, and it engages numerous partners in serving its varied functional roles. Small domains within the assembly recognize collectively a diverse range of macromolecules, including the core protein components, the cytoplasmic...

journal_title：Nucleic acids research

authors： Tsai YC,Du D,Domínguez-Malfavón L,Dimastrogiovanni D,Cross J,Callaghan AJ,García-Mena J,Luisi BF

更新日期：2012-11-01 00:00:00

abstract：:SITEHOUND-web (http://sitehound.sanchezlab.org) is a binding-site identification server powered by the SITEHOUND program. Given a protein structure in PDB format SITEHOUND-web will identify regions of the protein characterized by favorable interactions with a probe molecule. These regions correspond to putative ligand...

journal_title：Nucleic acids research

authors： Hernandez M,Ghersi D,Sanchez R

更新日期：2009-07-01 00:00:00

abstract：:Genomic DNA methylation profiles exhibit substantial variation within the human population, with important functional implications for gene regulation. So far little is known about the characteristics and determinants of DNA methylation variation among healthy individuals. We performed bioinformatic analysis of high-r...

journal_title：Nucleic acids research

authors： Bock C,Walter J,Paulsen M,Lengauer T

更新日期：2008-06-01 00:00:00