Distribution of 5-methylcytosine in the DNA of somatic and germline cells from bovine tissues.

abstract：:Unoxidized crystalline silicon, characterized by high purity, high homogeneity, sturdiness and an atomically flat surface, offers many advantages for the construction of electronic miniaturized biosensor arrays upon attachment of biomolecules (DNA, proteins or small organic compounds). This allows to study the inciden...

journal_title：Nucleic acids research

authors： Cattaruzza F,Cricenti A,Flamini A,Girasole M,Longo G,Prosperi T,Andreano G,Cellai L,Chirivino E

更新日期：2006-02-28 00:00:00

abstract：:Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...

journal_title：Nucleic acids research

authors： Han YH,Kim DK,Na TY,Ka NL,Choi HS,Lee MO

更新日期：2016-02-18 00:00:00

abstract：:The lytic-lysogenic decision in bacteriophage 186 is governed by the 186 CI repressor protein in a unique way. The 186 CI is proposed to form a wheel-like oligomer that can mediate either wrapped or looped nucleoprotein complexes to provide the cooperative and competitive interactions needed for regulation. Although c...

journal_title：Nucleic acids research

authors： Wang H,Dodd IB,Dunlap DD,Shearwin KE,Finzi L

更新日期：2013-06-01 00:00:00

abstract：:We report the development of a new database of technology services and products for analysis of biobank samples in biomedical research. BARCdb, the Biobanking Analysis Resource Catalogue (http://www.barcdb.org), is a freely available web resource, listing expertise and molecular resource capabilities of research centr...

journal_title：Nucleic acids research

authors： Galli J,Oelrich J,Taussig MJ,Andreasson U,Ortega-Paino E,Landegren U

更新日期：2015-01-01 00:00:00

abstract：:The IMGT/HLA database (http://www.ebi.ac.uk/imgt/hla) has provided a centralized repository for the sequences of the alleles named by the WHO Nomenclature Committee for Factors of the HLA System for the past four years. Since its initial release the database has grown and is the primary source of information for the s...

journal_title：Nucleic acids research

authors： Robinson J,Waller MJ,Parham P,de Groot N,Bontrop R,Kennedy LJ,Stoehr P,Marsh SG

更新日期：2003-01-01 00:00:00

abstract：:Identification of MHC-binding peptides is a prerequisite in rational design of T-cell based peptide vaccines. During the past decade a number of computational approaches have been introduced for the prediction of MHC-binding peptides, efficiently reducing the number of candidate binders that need to be experimentally ...

journal_title：Nucleic acids research

authors： Dönnes P,Kohlbacher O

更新日期：2006-07-01 00:00:00

abstract：:The nucleotide sequence of a Pseudomonas trans-zeatin producing gene (ptz) from the pCK1 plasmid of Pseudomonas syringae pv. savastanoi strain 1006 has been determined. This gene confers upon E. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respecti...

journal_title：Nucleic acids research

authors： Powell GK,Morris RO

更新日期：1986-03-25 00:00:00

abstract：:In this report, we provide an update on the services and content which are available on the web server of IBM's Bioinformatics and Pattern Discovery group. The server, which is operational around the clock, provides access to a large number of methods that have been developed and published by the group's members. Ther...

journal_title：Nucleic acids research

authors： Huynh T,Rigoutsos I

更新日期：2004-07-01 00:00:00

abstract：:We report the extended database and data mining resources newly released in the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). H-InvDB is a comprehensive annotation resource of human genes and transcripts, and consists of two main views and six sub-databases. The latest release of H-InvDB (release 6...

journal_title：Nucleic acids research

authors： Yamasaki C,Murakami K,Takeda J,Sato Y,Noda A,Sakate R,Habara T,Nakaoka H,Todokoro F,Matsuya A,Imanishi T,Gojobori T

更新日期：2010-01-01 00:00:00

abstract：:In many organisms (e.g., gram-positive eubacteria) Gin-tRNA is not formed by direct glutaminylation of tRNAGln but by a specific transamidation of Glu-tRNAGln. We wondered whether a similar transamidation pathway also operates in the formation of Asn-tRNA in these organisms. Therefore we tested in S-100 preparations o...

journal_title：Nucleic acids research

authors： Kim SI,Nalaskowska M,Germond JE,Pridmore D,Söll D

更新日期：1996-07-15 00:00:00

abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...

journal_title：Nucleic acids research

authors： Alva V,Nam SZ,Söding J,Lupas AN

更新日期：2016-07-08 00:00:00

abstract：:Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support before the 3'-ends are releas...

journal_title：Nucleic acids research

authors： Kwiatkowski M,Fredriksson S,Isaksson A,Nilsson M,Landegren U

更新日期：1999-12-15 00:00:00

abstract：:In eukaryotic ribosome, the N domain of polypeptide release factor eRF1 is involved in decoding stop signals in mRNAs. However, structure of the decoding site remains obscure. Here, we specifically altered the stop codon recognition pattern of human eRF1 by point mutagenesis of the invariant Glu55 and Tyr125 residues ...

journal_title：Nucleic acids research

authors： Kolosov P,Frolova L,Seit-Nebi A,Dubovaya V,Kononenko A,Oparina N,Justesen J,Efimov A,Kisselev L

更新日期：2005-11-10 00:00:00

abstract：:The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital importance for transcription, DNA replication and genome maintenance. Each of these activities is tightly regulated in both time and space. While we have a good understanding of chromatin organization in space, for example in fixe...

journal_title：Nucleic acids research

authors： Shaban HA,Seeber A

更新日期：2020-04-17 00:00:00

abstract：:Highly toxic DNA double-strand breaks (DSBs) readily trigger the DNA damage response (DDR) in cells, which delays cell cycle progression to ensure proper DSB repair. In Saccharomyces cerevisiae, mitotic S phase (20-30 min) is lengthened upon DNA damage. During meiosis, Spo11-induced DSB onset and repair lasts up to 5 ...

journal_title：Nucleic acids research

authors： Woo TT,Chuang CN,Higashide M,Shinohara A,Wang TF

更新日期：2020-09-04 00:00:00

abstract：:We previously reported that O4-alkyl dTTPs could replace, for short times, dTTP in polymer synthesis [Singer et al., PNAS 83, 26-32, 1986]. The reasons for such early termination of synthesis could be either proofreading or the eventual formation of weakly paired primer termini. Utilizing the known 3'----5' exonucleol...

journal_title：Nucleic acids research

authors： Singer B

更新日期：1986-08-26 00:00:00

abstract：:To bridge the gap between the rising information needs of biological and medical researchers and the rapidly growing number of online bioinformatics resources, we have created the Online Bioinformatics Resources Collection (OBRC) at the Health Sciences Library System (HSLS) at the University of Pittsburgh. The OBRC, c...

journal_title：Nucleic acids research

authors： Chen YB,Chattopadhyay A,Bergen P,Gadd C,Tannery N

更新日期：2007-01-01 00:00:00

abstract：:The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be...

journal_title：Nucleic acids research

authors： Galbraith AI,Chapleo MR,Itzhaki RF

更新日期：1978-09-01 00:00:00

abstract：:Enhancer-dependent transcription involving the promoter specificity factor σ(54) is widely distributed amongst bacteria and commonly associated with cell envelope function. For transcription initiation, σ(54)-RNA polymerase yields open promoter complexes through its remodelling by cognate AAA+ ATPase activators. Since...

journal_title：Nucleic acids research

authors： Schaefer J,Engl C,Zhang N,Lawton E,Buck M

更新日期：2015-09-03 00:00:00

abstract：:Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses T4 RNA ligase to attach a 3'-biotinylated donor molecule to target RNA. Microarray analyses indicate that directly labeled RNA is...

journal_title：Nucleic acids research

authors： Cole K,Truong V,Barone D,McGall G

更新日期：2004-06-17 00:00:00

abstract：:Post-transcriptional methylation of ribose at position O-2' is one of the most common and conserved types of RNA modification. Details of the functional roles of these methylations are far from clear, although in tRNA they are involved at position 34 in regulation of codon recognition and in eukaryotic rRNAs they are ...

journal_title：Nucleic acids research

authors： Qiu F,McCloskey JA

更新日期：1999-09-15 00:00:00

abstract：:The cancer drug daunomycin is an effective inhibitor of the B leads to Z transition in poly d(G-C) in 4 M NaCl. Both the rate and extent of the B leads to Z transition are decreased by the drug, as judged by equilibrium and kinetic studies. Daunomycin can, under some conditions, convert Z form DNA back to B form. Drug...

journal_title：Nucleic acids research

authors： Chaires JB

更新日期：1983-12-10 00:00:00

abstract：:Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...

journal_title：Nucleic acids research

authors： Yi TM,Walsh K,Schimmel P

更新日期：1991-06-11 00:00:00

abstract：:The hexamer (m5 dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R value of 15.6% at 1.3 A resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. ...

journal_title：Nucleic acids research

authors： Fujii S,Wang AH,van der Marel G,van Boom JH,Rich A

更新日期：1982-12-11 00:00:00

abstract：:Gene duplication is an important process in evolution. The availability of genome sequences of a number of organisms has made it possible to conduct comprehensive searches for duplicated genes enabling informative studies of their evolution. We have established the FGF (Fishing Gene Family) program to efficiently sear...

journal_title：Nucleic acids research

authors： Zheng H,Shi J,Fang X,Li Y,Vang S,Fan W,Wang J,Zhang Z,Wang W,Kristiansen K,Wang J

更新日期：2007-07-01 00:00:00

abstract：:The majority of genome-wide association study (GWAS) risk variants reside in non-coding DNA sequences. Understanding how these sequence modifications lead to transcriptional alterations and cell-to-cell variability can help unraveling genotype-phenotype relationships. Here, we describe a computational method, dubbed C...

journal_title：Nucleic acids research

authors： Li S,Alvarez RV,Sharan R,Landsman D,Ovcharenko I

更新日期：2017-03-17 00:00:00

abstract：:Alternative splicing (AS) and alternative polyadenylation (APA) generate diverse transcripts in mammalian genomes during development and differentiation. Epigenetic marks such as trimethylation of histone H3 lysine 36 (H3K36me3) and DNA methylation play a role in generating transcriptome diversity. Intragenic CpG isla...

journal_title：Nucleic acids research

authors： Amante SM,Montibus B,Cowley M,Barkas N,Setiadi J,Saadeh H,Giemza J,Contreras-Castillo S,Fleischanderl K,Schulz R,Oakey RJ

更新日期：2020-09-04 00:00:00

abstract：:Enzymes of intermediary metabolism are often reported to have moonlighting functions as RNA-binding proteins and have regulatory roles beyond their primary activities. Human serine hydroxymethyltransferase (SHMT) is essential for the one-carbon metabolism, which sustains growth and proliferation in normal and tumour c...

journal_title：Nucleic acids research

authors： Guiducci G,Paone A,Tramonti A,Giardina G,Rinaldo S,Bouzidi A,Magnifico MC,Marani M,Menendez JA,Fatica A,Macone A,Armaos A,Tartaglia GG,Contestabile R,Paiardini A,Cutruzzolà F

更新日期：2019-05-07 00:00:00

abstract：:The exact sites at which a number of drugs inhibit the nick translation of DNA by E.coli DNA polymerase-I have been pinpointed. In order to do this, a method has been developed for sequencing double-stranded plasmid DNA from the site of a specifically induced nick. The initial experiments have concentrated on analysis...

journal_title：Nucleic acids research

authors： Wilkins RJ

更新日期：1982-11-25 00:00:00

abstract：:Plant viruses ubiquitously mediate the induction of miR168 trough the activities of viral suppressors of RNA silencing (VSRs) controlling the accumulation of ARGONAUTE1 (AGO1), one of the main components of RNA silencing based host defence system. Here we used a mutant Tombusvirus p19 VSR (p19-3M) disabled in its main...

journal_title：Nucleic acids research

authors： Várallyay É,Oláh E,Havelda Z

更新日期：2014-01-01 00:00:00