Abstract:
:Genome length complementary DNA (cDNA) transcripts were synthesized in vitro by using purified virions of a cloned isolate of mouse sarcoma virus (MSV Clone 124). The cDNA transcripts were converted to double-stranded form by utilizing DNase-digested calf thymus DNA primers and E. coli DNA polymerase I. Restriction endonucleases Sal I, Hind III, Hpa I, Bgl II and Xba I were found to cleave the MSV double-stranded DNA once to generate two fragments, whereas restriction endonucleases Bgl I and Hae II cleaved twice to generate three fragments. Restriction endonucleases E. coli RI and Bam HI did not cleave MSV double-stranded DNA. The order of the restriction fragments was determined in relation to the 5' and 3' ends of the genomic RNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Verma IMdoi
10.1093/nar/6.5.1863subject
Has Abstractpub_date
1979-01-01 00:00:00pages
1863-7issue
5eissn
0305-1048issn
1362-4962journal_volume
6pub_type
杂志文章abstract::The early DNa products of reverse transcription have been analyzed from reconstructed reactions containing avian myeloblastosis virus 35S RNA . tRNAtrp complex and highly purified reverse transcriptase. We describe conditions for the synthesis of genome-length complementary DNA and two discrete species of plus strand ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.3.1009
更新日期:1982-02-11 00:00:00
abstract::We have isolated a rat cDNA, named FE65, hybridizing to an mRNA of about 2,300 nucleotides present in rat brain, undetectable in rat liver and very poorly represented in other tissues. An mRNA of the same size is present in human neuroblastoma cells and is absent from other human cell lines. The FE65 cDNA contains an ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.19.5269
更新日期:1991-10-11 00:00:00
abstract::Membraneless organelles (MOs) are dynamic liquid condensates that host a variety of specific cellular processes, such as ribosome biogenesis or RNA degradation. MOs form through liquid-liquid phase separation (LLPS), a process that relies on multivalent weak interactions of the constituent proteins and other macromole...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz848
更新日期:2020-01-08 00:00:00
abstract::Bacillus stearothermophilus large ribosomal subunits were reconstituted in the presence of 5S rRNAs from different origins and tested for their biological activities. The results obtained have shown that eubacterial and archaebacterial 5S rRNAs can easily substitute for B. stearothermophilus 5S rRNA in the reconstitut...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.8.3511
更新日期:1988-04-25 00:00:00
abstract::The proportion of lysine tRNA represented by the isoacceptor species lysine tRNA4 has previously been shown to be largest in cells with the greatest ability to proliferate. Using reverse phase chromatography (RPC-5), we have analyzed the changes in the relative quantities of lysine tRNA species which occur in differen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.3.851
更新日期:1978-03-01 00:00:00
abstract::DNA helicases are required for virtually every aspect of DNA metabolism, including replication, repair, recombination and transcription. A comprehensive description of these essential biochemical processes requires detailed understanding of helicase mechanisms. These enzymes are ubiquitous, having been identified in v...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkl501
更新日期:2006-01-01 00:00:00
abstract::The RESID Database is a comprehensive collection of annotations and structures for protein post-translational modifications including N-terminal, C-terminal and peptide chain cross-link modifications. The RESID Database includes systematic and frequently observed alternate names, Chemical Abstracts Service registry nu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.199
更新日期:2001-01-01 00:00:00
abstract::The transcriptional regulator Spx plays a key role in maintaining the redox homeostasis of Bacillus subtilis cells exposed to disulfide stress. Defects in Spx were previously shown to lead to differential expression of numerous genes but direct and indirect regulatory effects could not be distinguished. Here we identi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks755
更新日期:2012-10-01 00:00:00
abstract::An open reading frame upstream from nifHDK operon of Klebsiella pneumoniae had been described. The orientation of this open reading frame is opposite to that of nifHDK and sequence homology was found between the open reading frame promoter and the promoter of nifHDK operon. A recombinant plasmid carrying the promoter ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.12.4241
更新日期:1983-06-25 00:00:00
abstract::Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku130
更新日期:2014-04-01 00:00:00
abstract::Loss-of-function in melanocortin 1 receptor (MC1R), a GS protein-coupled receptor that regulates signal transduction through cAMP and protein kinase A (PKA) in melanocytes, is a major inherited melanoma risk factor. Herein, we report a novel cAMP-mediated response for sensing and responding to UV-induced DNA damage re...
journal_title:Nucleic acids research
pub_type: 杂志文章,收录出版
doi:10.1093/nar/gkw871
更新日期:2016-12-15 00:00:00
abstract::The function of non-coding RNA genes largely depends on their secondary structure and the interaction with other molecules. Thus, an accurate prediction of secondary structure and RNA-RNA interaction is essential for the understanding of biological roles and pathways associated with a specific RNA gene. We present web...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr248
更新日期:2011-07-01 00:00:00
abstract::The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to the target. To bridge this gap...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx688
更新日期:2017-10-13 00:00:00
abstract::A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5'-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5'-end of DNA. This linkage proved to be comp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg130
更新日期:2003-01-15 00:00:00
abstract::The secondary structure of V4, the largest variable area of eukaryotic small subunit ribosomal RNA, was re-examined by comparative analysis of 3253 nucleotide sequences distributed over the animal, plant and fungal kingdoms and a diverse set of protist taxa. An extensive search for compensating base pair substitutions...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.23.4698
更新日期:2000-12-01 00:00:00
abstract::The Arabidopsis Information Resource (TAIR, http://arabidopsis.org) is the model organism database for the fully sequenced and intensively studied model plant Arabidopsis thaliana. Data in TAIR is derived in large part from manual curation of the Arabidopsis research literature and direct submissions from the research...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm965
更新日期:2008-01-01 00:00:00
abstract::Strand Displacement Amplification (SDA) is an isothermal, in vitro method of amplifying a DNA target sequence prior to detection [Walker et al (1992) Nucleic Acids Res., 20, 1691-1693]. Here we describe a multiplex form of SDA that allows two target sequences and an internal amplification control to be co-amplified by...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.13.2670
更新日期:1994-07-11 00:00:00
abstract::Comparative genome hybridization (CGH) to DNA microarrays (array CGH) is a technique capable of detecting deletions and duplications in genomes at high resolution. However, array CGH studies of the human genome noting false negative and false positive results using large insert clones as probes have raised important c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki643
更新日期:2005-06-16 00:00:00
abstract::Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.10.3255
更新日期:1983-05-25 00:00:00
abstract::We investigate the mechanistic nature of the Snf2 family protein HARP, mutations of which are responsible for Schimke immuno-osseous dysplasia. Using a single-molecule magnetic tweezers assay, we construct RPA-stabilized DNA bubbles within torsionally constrained DNA to investigate the annealing action of HARP on a ph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx147
更新日期:2017-05-05 00:00:00
abstract::Homologous recombination provides a means for the in vivo construction of recombinant DNA molecules that may be problematic to assemble in vitro. We have investigated the efficiency of recombination within the Caenorhabditis elegans germ line as a function of the length of homology between recombining molecules. Our f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm857
更新日期:2007-01-01 00:00:00
abstract::Magnetic tweezers are a powerful single-molecule technique that allows real-time quantitative investigation of biomolecular processes under applied force. High pulling forces exceeding tens of picoNewtons may be required, e.g. to probe the force range of proteins that actively transcribe or package the genome. Frequen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku677
更新日期:2014-10-01 00:00:00
abstract::Tcf/Lef family transcription factors are the downstream effectors of the Wingless/Wnt signal transduction pathway. Upon Wingless/Wnt signalling, beta-catenin translocates to the nucleus, interacts with Tcf (1-3) and thus activates transcription of target genes (4,5). Tcf factors also interact with members of the Grouc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.7.1410
更新日期:2001-04-01 00:00:00
abstract::Lnc2Meth (http://www.bio-bigdata.com/Lnc2Meth/), an interactive resource to identify regulatory relationships between human long non-coding RNAs (lncRNAs) and DNA methylation, is not only a manually curated collection and annotation of experimentally supported lncRNAs-DNA methylation associations but also a platform t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx985
更新日期:2018-01-04 00:00:00
abstract::During DNA replication replicative polymerases move in discrete mechanical steps along the DNA template. To address how the chemical cycle is coupled to mechanical motion of the enzyme, here we use optical tweezers to study the translocation mechanism of individual bacteriophage Phi29 DNA polymerases during processive...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv204
更新日期:2015-04-20 00:00:00
abstract::Two new endonuclease activities, endonuclease B and endonuclease C, obtained from yeast nuclear preparations have been separated and partially characterized. Endonuclease B has a primary requirement for Mn2+ which cannot be replaced by Mg2+ or Ca2+, and makes single-strand scissions in double-stranded DNA. Endonculeas...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.7.1023
更新日期:1975-07-01 00:00:00
abstract::Since 2010, the Genomicus web server is available online at http://genomicus.biologie.ens.fr/genomicus. This graphical browser provides access to comparative genomic analyses in four different phyla (Vertebrate, Plants, Fungi, and non vertebrate Metazoans). Users can analyse genomic information from extant species, as...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1003
更新日期:2018-01-04 00:00:00
abstract::Bacterial Rho-independent terminators (RITs) are important genomic landmarks involved in gene regulation and terminating gene expression. In this investigation we present RNIE, a probabilistic approach for predicting RITs. The method is based upon covariance models which have been known for many years to be the most a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr168
更新日期:2011-08-01 00:00:00
abstract::The -1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome corona...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki731
更新日期:2005-07-29 00:00:00
abstract::Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.10.1911
更新日期:1975-10-01 00:00:00