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Hybridization of labeled RNA to DNA in agarose gels.

Abstract：

:Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel allows the identification of the DNA fragment which is complementary to the RNA probe. The technique is rapid, easy and inexpensive. It is sensitive enough to detect individual genes in a mixture of fragments produced by restriction enzyme digestion of complex cellular DNA. We have used this technique to determine which of the Hin III and Eco R1 fragments of phi80d3ilv+su+7 and E. coli DNAs contain the 5S, 16S and 23S ribosomal RNA (rRNA) genes of E. coli.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Shinnick TM,Lund E,Smithies O,Blattner FR

doi

10.1093/nar/2.10.1911

keywords:

subject

Has Abstract

pub_date

1975-10-01 00:00:00

pages

1911-29

issue

10

eissn

0305-1048

issn

1362-4962

journal_volume

2

pub_type

杂志文章

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