The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context.

abstract：:Reactive oxygen species (ROS) have been implicated as the cause of cumulative damage to DNA, proteins and lipids that can ultimately result in cell death. A common problem when measuring oxidative DNA damage has been the introduction of modifications in the native state of the molecule by many DNA isolation methods. W...

journal_title：Nucleic acids research

authors： Milano J,Day BJ

更新日期：2000-02-15 00:00:00

abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...

journal_title：Nucleic acids research

authors： Saintomé C,Amrane S,Mergny JL,Alberti P

更新日期：2016-04-07 00:00:00

abstract：:We have compiled the DNA sequence data for E. coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the fifth listing replacing and increasing the former listings substantially. However, in order to save space this printed version contains...

journal_title：Nucleic acids research

authors： Kröger M,Wahl R,Rice P

更新日期：1993-07-01 00:00:00

abstract：:CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to ...

journal_title：Nucleic acids research

authors： Kiliszek A,Kierzek R,Krzyzosiak WJ,Rypniewski W

更新日期：2010-12-01 00:00:00

abstract：:The Department of Energy (DOE) Joint Genome Institute (JGI) is a national user facility with massive-scale DNA sequencing and analysis capabilities dedicated to advancing genomics for bioenergy and environmental applications. Beyond generating tens of trillions of DNA bases annually, the Institute develops and maintai...

journal_title：Nucleic acids research

authors： Grigoriev IV,Nordberg H,Shabalov I,Aerts A,Cantor M,Goodstein D,Kuo A,Minovitsky S,Nikitin R,Ohm RA,Otillar R,Poliakov A,Ratnere I,Riley R,Smirnova T,Rokhsar D,Dubchak I

更新日期：2012-01-01 00:00:00

abstract：:The presence of the C.C mispair in a defined duplex oligodeoxynucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5C...

journal_title：Nucleic acids research

authors： Smith SS,Hardy TA,Baker DJ

更新日期：1987-09-11 00:00:00

abstract：:Alternative splicing (AS) of pre-mRNAs is an important regulatory mechanism shaping the transcriptome. In plants, only few RNA-binding proteins are known to affect AS. Here, we show that the glycine-rich RNA-binding protein AtGRP7 influences AS in Arabidopsis thaliana. Using a high-resolution RT-PCR-based AS panel, we...

journal_title：Nucleic acids research

authors： Streitner C,Köster T,Simpson CG,Shaw P,Danisman S,Brown JW,Staiger D

更新日期：2012-12-01 00:00:00

abstract：:The structure in solution of an RNA fragment (218 nucleotides long) containing part of E. coli 16S rRNA domain 2 has been studied using the intrinsic photoaffinity probe 4-thiouridine (s4U). In vitro transcription with T7 polymerase, in the presence of s4U triphosphate yielded complete RNA molecules. An affinity elect...

journal_title：Nucleic acids research

authors： Dubreuil YL,Expert-Bezançon A,Favre A

更新日期：1991-07-11 00:00:00

abstract：:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes an...

journal_title：Nucleic acids research

authors： Wang ML,Leitch AR,Schwarzacher T,Heslop-Harrison JS,Moore G

更新日期：1992-04-25 00:00:00

abstract：:We describe a versatile and extensible integrated bioinformatics toolkit for the analysis of biological sequences over the Internet. The web portal offers convenient interactive access to a growing pool of chainable bioinformatics software tools and databases that are centrally installed and maintained by the RZG. Cur...

journal_title：Nucleic acids research

authors： Rampp M,Soddemann T,Lederer H

更新日期：2006-07-01 00:00:00

abstract：:A novel, site-specific, DNA backbone S-modification (phosphorothioation) has been discovered, but its in vivo function(s) have remained obscure. Here, we report that the enteropathogenic Salmonella enterica serovar Cerro 87, which possesses S-modified DNA, restricts DNA isolated from Escherichia coli, while protecting...

journal_title：Nucleic acids research

authors： Xu T,Yao F,Zhou X,Deng Z,You D

更新日期：2010-11-01 00:00:00

abstract：:One of the obstacles hindering a better understanding of cancer is its heterogeneity. However, computational approaches to model cancer heterogeneity have lagged behind. To bridge this gap, we have developed a new probabilistic approach that models individual cancer cases as mixtures of subtypes. Our approach can be s...

journal_title：Nucleic acids research

authors： Cho DY,Przytycka TM

更新日期：2013-09-01 00:00:00

abstract：:The Aspergillus Genome Database (AspGD) is an online genomics resource for researchers studying the genetics and molecular biology of the Aspergilli. AspGD combines high-quality manual curation of the experimental scientific literature examining the genetics and molecular biology of Aspergilli, cutting-edge comparativ...

journal_title：Nucleic acids research

authors： Arnaud MB,Chibucos MC,Costanzo MC,Crabtree J,Inglis DO,Lotia A,Orvis J,Shah P,Skrzypek MS,Binkley G,Miyasato SR,Wortman JR,Sherlock G

更新日期：2010-01-01 00:00:00

abstract：:The transactivator Staf, which contains seven contiguous zinc fingers of the C(2)-H(2)type, exerts its effects on gene expression by binding to specific targets in vertebrate small nuclear RNA (snRNA) and snRNA-type gene promoters. Here, we have investigated the interaction of the Staf zinc finger domain with the opti...

journal_title：Nucleic acids research

authors： Schaub M,Krol A,Carbon P

更新日期：2000-05-15 00:00:00

abstract：:The frequencies of occurrence of four bases in the first, second and third codon positions and in the total coding sequences have been calculated by the codon usage table published in 1990 by Ikemura et al. The distribution of frequencies are further analysed in detail by a graphic technique presented recently by us. ...

journal_title：Nucleic acids research

authors： Zhang CT,Zhang R

更新日期：1991-11-25 00:00:00

abstract：:Homeodomains appear to be one of the most frequently employed DNA-binding domains in a superfamily of transacting factors. It is likely that during evolution several sub-types of homeodomain have evolved from a common ancestral domain, resulting in distinct but closely related DNA-binding preferences. Here we describe...

journal_title：Nucleic acids research

authors： Jagla K,Stanceva I,Dretzen G,Bellard F,Bellard M

更新日期：1994-04-11 00:00:00

abstract：:Identification of common sub-sequences for a group of functionally related DNA sequences can shed light on the role of such elements in cell-specific gene expression. In the megakaryocytic lineage, no one single unique transcription factor was described as linage specific, raising the possibility that a cluster of gen...

journal_title：Nucleic acids research

authors： Hazony Y,Lu J,St Hilaire C,Ravid K

更新日期：2006-01-01 00:00:00

abstract：:Riboswitch RNAs fold into complex tertiary structures upon binding to their cognate ligand. Ligand recognition is accomplished by key residues in the binding pocket. In addition, it often crucially depends on the stability of peripheral structural elements. The ligand-bound complex of the guanine-sensing riboswitch fr...

journal_title：Nucleic acids research

authors： Buck J,Wacker A,Warkentin E,Wöhnert J,Wirmer-Bartoschek J,Schwalbe H

更新日期：2011-12-01 00:00:00

abstract：:Cooperative hybridization has been used to establish physical linkage between two loci on a DNA strand. Linkage was detected by hybridization to a new type of high-density oligonucleotide array. Each synthesis location on the array contains a mixture of two different probe sequences. Each of the two probes can hybridi...

journal_title：Nucleic acids research

authors： Gentalen E,Chee M

更新日期：1999-03-15 00:00:00

abstract：:Multicellular organismal development is controlled by a complex network of transcription factors, promoters and enhancers. Although reliable computational and experimental methods exist for enhancer detection, prediction of their target genes remains a major challenge. On the basis of available literature and ChIP-seq...

journal_title：Nucleic acids research

authors： Rödelsperger C,Guo G,Kolanczyk M,Pletschacher A,Köhler S,Bauer S,Schulz MH,Robinson PN

更新日期：2011-04-01 00:00:00

abstract：:MCScan is an algorithm able to scan multiple genomes or subgenomes in order to identify putative homologous chromosomal regions, and align these regions using genes as anchors. The MCScanX toolkit implements an adjusted MCScan algorithm for detection of synteny and collinearity that extends the original software by in...

journal_title：Nucleic acids research

authors： Wang Y,Tang H,Debarry JD,Tan X,Li J,Wang X,Lee TH,Jin H,Marler B,Guo H,Kissinger JC,Paterson AH

更新日期：2012-04-01 00:00:00

abstract：:The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We report here that these transcripts produce three forms of MTH1 polypeptides (...

journal_title：Nucleic acids research

authors： Oda H,Taketomi A,Maruyama R,Itoh R,Nishioka K,Yakushiji H,Suzuki T,Sekiguchi M,Nakabeppu Y

更新日期：1999-11-15 00:00:00

abstract：:DNA synthesized by in vitro transcription on rat interferon (IFN) mRNA has been cloned and amplified as recombinant DNA. The nucleotide sequence of these rat IFN cDNA clones revealed i. the partial presence of the coding region of the gene and ii all cDNA clones were derived from the same subtype of rat IFN-alpha mRNA...

journal_title：Nucleic acids research

authors： Dijkema R,Pouwels P,de Reus A,Schellekens H

更新日期：1984-01-25 00:00:00

abstract：:Wild type p53 assembles into a latent multiprotein complex which can be activated for sequence-specific DNA binding in vitro by proteins targeting the carboxy-terminal domain. Using an optimized system coupling the post-translational modification of wild type p53 to activation of sequence specific DNA binding, we exam...

journal_title：Nucleic acids research

authors： Hupp TR,Meek DW,Midgley CA,Lane DP

更新日期：1993-07-11 00:00:00

abstract：:Enzymes of intermediary metabolism are often reported to have moonlighting functions as RNA-binding proteins and have regulatory roles beyond their primary activities. Human serine hydroxymethyltransferase (SHMT) is essential for the one-carbon metabolism, which sustains growth and proliferation in normal and tumour c...

journal_title：Nucleic acids research

authors： Guiducci G,Paone A,Tramonti A,Giardina G,Rinaldo S,Bouzidi A,Magnifico MC,Marani M,Menendez JA,Fatica A,Macone A,Armaos A,Tartaglia GG,Contestabile R,Paiardini A,Cutruzzolà F

更新日期：2019-05-07 00:00:00

abstract：:We have isolated the gene coding for the U2 analogue in trypanosomes. The 148 nucleotide long U2 RNA is capped and transcribed from a single copy gene. The 5' half of the molecule is highly homologous to mammalian U2 RNA, while the 3' half does not show any significant sequence homology with the mammalian counterpart....

journal_title：Nucleic acids research

authors： Tschudi C,Richards FF,Ullu E

更新日期：1986-11-25 00:00:00

abstract：:Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...

journal_title：Nucleic acids research

authors： Voltz K,Léonard J,Touceda PT,Conyard J,Chaker Z,Dejaegere A,Godet J,Mély Y,Haacke S,Stote RH

更新日期：2016-04-20 00:00:00

abstract：:In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneou...

journal_title：Nucleic acids research

authors： Gambaryan AS,Venkstern TV,Bayev AA

更新日期：1976-08-01 00:00:00

abstract：:DNA molecules with covalently sealed (hairpin) ends are probable intermediates in V(D)J recombination. According to current models hairpin ends are opened to produce short single-stranded extensions that are thought to be precursors of a particular type of extra nucleotides, termed P nucleotides, which are frequently ...

journal_title：Nucleic acids research

authors： Kabotyanski EB,Zhu C,Kallick DA,Roth DB

更新日期：1995-10-11 00:00:00

abstract：:The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. Th...

journal_title：Nucleic acids research

authors： Lackner KJ,Law SW,Brewer HB Jr

更新日期：1985-06-25 00:00:00