Abstract:
:The tissue-specific expression of the Drosophila beta 2 tubulin gene ( B2t ) is accomplished by the action of a 14-bp activator element (beta2UE1) in combination with certain regulatory elements of the TATA-less, Inr-containing B2t core promoter. We performed an in vivo analysis of the Inr element function in the B2t core promoter using a transgenic approach. Our experiments demonstrate that the Inr element acts as a functional cis -regulatory element in vivo and quantitatively regulates tissue-specific reporter expression in transgenic animals. However, our mutational analysis of the Inr element demonstrates no essential role of the Inr in mediating tissue specificity of the B2t promoter. In addition, a downstream element seems to affect promoter activity in combination with the Inr. In summary, our data show for the first time the functionality of the Inr element in an in vivo background situation in Drosophila.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Santel A,Kaufmann J,Hyland R,Renkawitz-Pohl Rdoi
10.1093/nar/28.6.1439keywords:
subject
Has Abstractpub_date
2000-03-15 00:00:00pages
1439-46issue
6eissn
0305-1048issn
1362-4962pii
gkd258journal_volume
28pub_type
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