Abstract:
:The interaction specificity of salmon sperm DNA with 2-hydroxyethanethionlato(2,2',2''-terpyridine)platinum(II),PtTS has been studied. The results of 1H and 13C nuclear magnetic resonance, flow dichroism and circular dichroism studies are found to be consistent with an intercalation mode of binding as has been proposed earlier by lippard and coworkers.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Gabbay EJ,Adkins MA,Yen Sdoi
10.1093/nar/7.4.1081subject
Has Abstractpub_date
1979-10-25 00:00:00pages
1081-90issue
4eissn
0305-1048issn
1362-4962journal_volume
7pub_type
杂志文章abstract::Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp554
更新日期:2009-09-01 00:00:00
abstract::The nucleotide sequence of a Pseudomonas trans-zeatin producing gene (ptz) from the pCK1 plasmid of Pseudomonas syringae pv. savastanoi strain 1006 has been determined. This gene confers upon E. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respecti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.6.2555
更新日期:1986-03-25 00:00:00
abstract::MS²PIP is a data-driven tool that accurately predicts peak intensities for a given peptide's fragmentation mass spectrum. Since the release of the MS²PIP web server in 2015, we have brought significant updates to both the tool and the web server. In addition to the original models for CID and HCD fragmentation, we hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz299
更新日期:2019-07-02 00:00:00
abstract::The H19 lncRNA has been implicated in development and growth control and is associated with human genetic disorders and cancer. Acting as a molecular sponge, H19 inhibits microRNA (miRNA) let-7. Here we report that H19 is significantly decreased in muscle of human subjects with type-2 diabetes and insulin resistant ro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1160
更新日期:2014-12-16 00:00:00
abstract::Many, if not all, mRNAs in T.brucei start with the same sequence of 35 nucleotides, separately encoded in clustered so-called mini-exon repeats. From these mini-exon repeats a 141-nt precursor RNA with the 35-nt sequence at its 5' end is transcribed. Indirect evidence suggests that this RNA is linked in a second step ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.24.9457
更新日期:1984-12-21 00:00:00
abstract::Three-dimensional protein structures provide invaluable information for understanding and regulating biological functions of proteins. The GalaxyWEB server predicts protein structure from sequence by template-based modeling and refines loop or terminus regions by ab initio modeling. This web server is based on the met...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks493
更新日期:2012-07-01 00:00:00
abstract::PUF proteins, named for Drosophila Pumilio (PUM) and Caenorhabditis elegans fem-3-binding factor (FBF), recognize specific sequences in the mRNAs they bind and control. RNA binding by classical PUF proteins is mediated by a characteristic PUM homology domain (PUM-HD). The Puf1 and Puf2 proteins possess a distinct arch...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz583
更新日期:2019-09-19 00:00:00
abstract::Over millions of years the structure and complexity of the transcriptional regulatory network (TRN) in bacteria has changed, reorganized and enabled them to adapt to almost every environmental niche on earth. In order to understand the plasticity of TRNs in bacteria, we studied the conservation of currently known TRNs...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl423
更新日期:2006-07-13 00:00:00
abstract::Double-strand breaks (DSBs) are dangerous chromosomal lesions that must be efficiently repaired in order to avoid loss of genetic information or cell death. In all organisms studied to date, two different mechanisms are used to repair DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Previous ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm521
更新日期:2007-01-01 00:00:00
abstract::The nature and pace of genome mutation is largely unknown. Because standard methods sequence DNA from populations of cells, the genetic composition of individual cells is lost, de novo mutations in cells are concealed within the bulk signal and per cell cycle mutation rates and mechanisms remain elusive. Although sing...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt345
更新日期:2013-07-01 00:00:00
abstract::The Metabolic Pathway Collection from EMP is an extraction of data from the larger Enzymes and Metabolic Pathways database (EMP). This extraction has been made publicly available in the hope that others will find it useful for a variety of purposes. The original release in October 1995 contained 1814 distinct pathways...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.1.37
更新日期:1997-01-01 00:00:00
abstract::Cellular senescence, an integral component of aging and cancer, arises in response to diverse triggers, including telomere attrition, macromolecular damage and signaling from activated oncogenes. At present, senescent cells are identified by the combined presence of multiple traits, such as senescence-associated prote...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz555
更新日期:2019-08-22 00:00:00
abstract::The 2'-5' oligoadenylate synthetases form a well conserved family of interferon induced proteins, presumably present throughout the mammalian class. Using the Expressed Sequence Tag databases, we have identified a novel member of this family. This protein, which we named p59 2'-5' oligoadenylate synthetase-like protei...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.18.4121
更新日期:1998-09-15 00:00:00
abstract::The chromatographic profiles of isoaccepting tRNAs were analyzed at five time points during the 96 hr, dimethylsulfoxide induced, erythroid-like differentiation of Friend leukemia cells. Sixty-four isoaccepting species of tRNA for 16 amino acids were resolved by RPC-5 chromatography. The relative amounts of tRNAphe, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.15.3467
更新日期:1980-08-11 00:00:00
abstract::The NHGRI-EBI GWAS Catalog has provided data from published genome-wide association studies since 2008. In 2015, the database was redesigned and relocated to EMBL-EBI. The new infrastructure includes a new graphical user interface (www.ebi.ac.uk/gwas/), ontology supported search functionality and an improved curation ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1133
更新日期:2017-01-04 00:00:00
abstract::Mono- and polyclonal antibodies directed against different domains of the potato leafroll luteovirus (PLRV) P1 (ORF1) protein were applied to the analysis of P1 expression during PLRV replication in planta. Western analyses detected P1 and a protein of approximately 25 kDa (P1-C25) that accumulated to readily detectab...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.2.421
更新日期:1999-01-15 00:00:00
abstract::In spermidine-condensed calf thymus DNA preparations, torus-shaped condensates were shown by transmission electron microscopy to exist under the hydrated conditions of the freeze fracture experiment. Using extremely low Pt metal deposition levels (9 A Pt/C) high-contrast replicas of the spermidine-DNA toruses were obt...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.6.1839
更新日期:1983-03-25 00:00:00
abstract::We have recently shown that phosphorothioate (PS) oligodeoxynucleotide (ODN) analogs, unlike their normal congeners, exhibit significant anti-HIV activity (Matsukura et al., (1987) Proc. Natl. Acad. Sci. USA 84, 7706-7710). We now report the syntheses, melting temperatures (Tm), and nuclease susceptibilities of a seri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.8.3209
更新日期:1988-04-25 00:00:00
abstract::Many types of DNA structures are generated in response to DNA damage, repair and recombination that require processing via specialized nucleases. DNA hairpins represent one such class of structures formed during V(D)J recombination, palindrome extrusion, DNA transposition and some types of double-strand breaks. Here w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1059
更新日期:2012-03-01 00:00:00
abstract::The three-dimensional Poisson-Boltzmann equation for the distribution of counterion charge density around double helical DNA has been solved by an iterative procedure. These computations have been performed for 0.01M monovalent salt solutions. A systematic study of the ten possible sequences found among adjacent nucle...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.3.1479
更新日期:1986-02-11 00:00:00
abstract::Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphates using polynucleotide phosphorylase from Escherichia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single strand...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.7.1675
更新日期:1980-04-11 00:00:00
abstract::A minizyme is a hammerhead ribozyme with short oligonucleotide linkers instead of stem-loop II. In a previous study we demonstrated that a minizyme with high activity forms a dimeric structure with a common stem II. Because of their dimeric structure, minizymes are potentially capable of cleaving a substrate at two di...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.12.2302
更新日期:1996-06-15 00:00:00
abstract::Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky381
更新日期:2018-07-02 00:00:00
abstract::In prokaryotes and archaea transfer ribonucleic acid (tRNA) stability as well as cellular UV protection relies on the post-transcriptional modification of uracil at position 8 (U8) of tRNAs by the 4-thiouridine synthetase ThiI. Here, we report three crystal structures of ThiI from Thermotoga maritima in complex with a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku249
更新日期:2014-06-01 00:00:00
abstract::RNA molecules fold into characteristic secondary structures for their diverse functional activities such as post-translational regulation of gene expression. Searching homologs of a pre-defined RNA structural motif, which may be a known functional element or a putative RNA structural motif, can provide useful informat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl231
更新日期:2006-07-01 00:00:00
abstract::Vascular endothelial growth factor A (VEGF-A) is a master regulator of angiogenesis, vascular development and function. In this study we investigated the transcriptional regulation of VEGF-A-responsive genes in primary human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using gen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1036
更新日期:2014-11-10 00:00:00
abstract::Mouse embryo fibroblasts (MEFs) are convenient sources for biochemical studies when cell number in mouse embryos is limiting. To derive the imprinting signature of MEFs and potentially detect novel imprinted genes we performed strand- and allele-specific RNA deep sequencing. We used sequenom allelotyping in embryo and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1042
更新日期:2014-02-01 00:00:00
abstract::Structural domains are considered as the basic units of protein folding, evolution, function and design. Automatic decomposition of protein structures into structural domains, though after many years of investigation, remains a challenging and unsolved problem. Manual inspection still plays a key role in domain decomp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg189
更新日期:2003-02-01 00:00:00
abstract::Regulation of mRNA translation is an important mechanism determining the level of expression of proteins in eukaryotic cells. Translation is most commonly initiated by cap-dependent scanning, but many eukaryotic mRNAs contain internal ribosome entry segments (IRESs), providing an alternative means of initiation capabl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp623
更新日期:2009-09-01 00:00:00
abstract::Laser-Raman spectra of poly(rI) show the formation of an ordered complex in aqueous solutions of high ionic strength. This structure exhibits the A-helix geometry, contains stacked bases and is apparently stabilized by specific hydrogen bonding involving hypoxanthine C6=0 groups. Thermal dissociation of the poly(rI) c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.7.2407
更新日期:1977-07-01 00:00:00